Skip to main content

Biotin conjugation / labeling in < 20 mins with 30 secs hands-on time using Biotinylation Kit / Biotin Conjugation Kit (Fast, Type B) - Lightning-Link® ab201796. 100% antibody recovery

- Cited in over 15 publications
- Biotin labeled antibody is ready for use in applications like WB, ELISA, and IHC, with no need for further purification
- Compatible with most standard antibody formulations
- Scalable;use the same conjugation method from 10µg-100mg of antibody
- Use Lightning-Link® conjugation with over 45 labels, including Alexa Fluor®, DyLight®, tandem dyes, enzymes, oligonucleotides, and gold nanoparticles


Images

Conjugation - Biotinylation Kit / Biotin Conjugation Kit (Fast, Type B) - Lightning-Link® (AB201796), expandable thumbnail
  • Conjugation - Biotinylation Kit / Biotin Conjugation Kit (Fast, Type B) - Lightning-Link® (AB201796), expandable thumbnail

Publications

Associated Products

Select an associated product type

1 product for Alternative Product

What's included?

3 x 10 µg
Components
Biotin (Type B) Conjugation Mix
3 x 10 µg
Modifier reagent
1 x 200 µL
Quencher reagent
1 x 200 µL

Recommended products

Biotin conjugation / labeling in < 20 mins with 30 secs hands-on time using Biotinylation Kit / Biotin Conjugation Kit (Fast, Type B) - Lightning-Link® ab201796. 100% antibody recovery

- Cited in over 15 publications
- Biotin labeled antibody is ready for use in applications like WB, ELISA, and IHC, with no need for further purification
- Compatible with most standard antibody formulations
- Scalable;use the same conjugation method from 10µg-100mg of antibody
- Use Lightning-Link® conjugation with over 45 labels, including Alexa Fluor®, DyLight®, tandem dyes, enzymes, oligonucleotides, and gold nanoparticles

Storage

Shipped at conditions
Ambient - Cannot Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Biotinylation Kit / Biotin Conjugation Kit ab201796 uses a simple and quick process for biotinylation / biotin labeling of antibodies. It can also be used to conjugate other proteins or peptides. Learn about our antibody labeling kits and their advantages.

To conjugate an antibody to Biotin using this kit:
- add modifier to antibody and incubate for 15 mins
- add quencher and incubate for 5 mins
The conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.

Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to Biotin.

The Type B Biotinylation Kit / Biotin Conjugation Kit is optimized to produce conjugates for assays in which the biotinylated protein is captured by streptavidin immobilized on a surface (e.g., plates, nitrocellulose, magnetic beads etc).

Use the Type A Biotinylation Kit / Biotin Conjugation Kit Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® ab201795 to produce conjugates for assays in which a streptavidin-labeled detection reagent will be used.

Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.

This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link® Rapid Biotin Type B Labeling Kit. 371-0005 is the same as the 100 μg size. 371-0010 is the same as the 3 x 100 ug size. 371-0030 is the same as the 3 x 10 ug size. 371-0015 is the same as the 1 mg size.

Amount and volume of antibody for conjugation to Biotin

Kit sizeRecommended
amount of antibody1		Maximum
amount of antibody		Maximum antibody
volume2
3 x 10 μg3 x 10 μg3 x 20 μg3 x 10 μL
100 μg1 x 100 μg1 x 200 μg1 x 100 μL
3 x 100 μg3 x 100 μg3 x 200 μg3 x 100 μL
1 x 1 mg1 x 1 mg1 x 2 mg1 x 1 mL

1 Using the maximum amount of antibody may result in less labelling per antibody.

2 Ideal antibody concentration is 1 mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 2 mg/ml or < 0.5 mg/ml should be diluted /concentrated.

Buffer Requirements for Conjugation

Buffer should be pH 6.5-8.5.

Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.

50mM / 0.6% Tris10.1% BSA250% glycerol
0.1% sodium azidePBSPotassium phosphate
Sodium chlorideHEPESSucrose
Sodium citrateEDTATrehalose

1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
2 BSA can also interfere with the use of the conjugated antibody in tissue staining.

Incompatible buffer constituents

ThiomerosalProclinGlycine
ArginineGlutathioneDTT

If a constituent of the buffer containing your antibody or protein is not listed above, please contact us.

Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.

Storing and handling conjugation kits

Lyophilized Lightning-Link® components are hygroscopic.

Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Conjugation - Biotinylation Kit / Biotin Conjugation Kit (Fast, Type B) - Lightning-Link® (ab201796), expandable thumbnail
    Image from Parkash, Om, et al., Diagnostics (Basel), 11(1):33; doi: 10.3390/diagnostics11010033. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    Conjugation - Biotinylation Kit / Biotin Conjugation Kit (Fast, Type B) - Lightning-Link® (ab201796)

    Parkash, Om, et al used Biotinylation Kit / Biotin Conjugation Kit (Fast, Type B) - Lightning-Link® (ab201796) as part of the development and evaluation of a biosensor based on screen-printed carbon electrodes (SPCEs) for the detection of dengue-specific immunoglobulin M (IgM) antibodies. They used the kit to conjugate Biotinylation Kit / Biotin to anti-Human IgM antibody for use in conjugation.
    (A) Schematic diagram of the screen printed carbon electrode (SCPE)-based dengue IgM biosensor. The biosensor was constructed by sequentially adding optimised concentration of anti-human IgM capture antibody, blocking agent, human IgM antibody or serum sample, dengue antigen and detection antibody, with washing steps in between. Electrochemical signal was generated following addition of TMB substrate. (B) Comparison of various immobilisation techniques for the goat anti-human IgM capture antibody. NC: negative control consisting of a dengue IgM negative serum sample; PC: dengue IgM positive serum sample. (C) Field Emission Scanning Electron Microscopy (FESEM) surface images of (left panel) a bare carbon electrode and (right panel) a carbon electrode modified with an anti-human IgM antibody using the streptavidin/biotin immobilisation system. Both capture and detection antibodies were labeled using Lightning-Link® conjugation kits (ab201796 and HRP Conjugation Kit - Lightning-Link® ab102890).

  • Conjugation - Biotinylation Kit / Biotin Conjugation Kit (Fast, Type B) - Lightning-Link® (ab201796), expandable thumbnail
    Image from Rackus et al., Lab Chip., 17(13):2272-2280; doi: 10.1039/c7lc00440k. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/3.0/

    Conjugation - Biotinylation Kit / Biotin Conjugation Kit (Fast, Type B) - Lightning-Link® (ab201796)

    Rackus, Darius G., et al used Biotinylation Kit / Biotin Conjugation Kit (Type B) - Lightning-Link® (ab201796) as part of examining digital microfluidics for detection a malaria biomarker. They used the kit to conjugate Biotinylation Kit / Biotin to anti-Plasmodium falciparum LDH antibody for use in pre-concentration by liquid intake by paper (P-CLIP).
    DMF immunoassay for PfLDH. Comparison of mean signals obtained by traditional DMF-ELISA (black) and P-CLIP modified DMF-ELISA (grey) for concentrations below the limit of detection (7 ng mL-1) and limit of quantitation (70 ng mL-1) of the traditional DMF-ELISA. Error bars ± 1 std. dev. (n = 3).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com