Biotinylation Kit / Biotin Conjugation Kit (Fast, Type B) - Lightning-Link®
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(1 Review)
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(25 Publications)
- Rapid Conjugation: achieve Biotin labeling in under 20 minutes with just 30 seconds of hands-on time.
- High Efficiency: ensures 100% antibody recovery, meaning no loss of valuable antibodies.
- Versatility: optimized to produce conjugates for assays where biotinylated protein is captured by streptavidin immobilized on a surface.
- Confidence: cited in over 20 publications.
- Conjugation
PubMed
Conjugation - Biotinylation Kit / Biotin Conjugation Kit (Fast, Type B) - Lightning-Link® (AB201796)
Parkash, Om, et al used Biotinylation Kit / Biotin Conjugation Kit (Fast, Type B) - Lightning-Link® (ab201796) as part of the development and evaluation of a biosensor based on screen-printed carbon electrodes (SPCEs) for the detection of dengue-specific immunoglobulin M (IgM) antibodies. They used the kit to conjugate Biotinylation Kit / Biotin to anti-Human IgM antibody for use in conjugation.
(A) Schematic diagram of the screen printed carbon electrode (SCPE)-based dengue IgM biosensor. The biosensor was constructed by sequentially adding optimised concentration of anti-human IgM capture antibody, blocking agent, human IgM antibody or serum sample, dengue antigen and detection antibody, with washing steps in between. Electrochemical signal was generated following addition of TMB substrate. (B) Comparison of various immobilisation techniques for the goat anti-human IgM capture antibody. NC : negative control consisting of a dengue IgM negative serum sample; PC : dengue IgM positive serum sample. (C) Field Emission Scanning Electron Microscopy (FESEM) surface images of (left panel) a bare carbon electrode and (right panel) a carbon electrode modified with an anti-human IgM antibody using the streptavidin/biotin immobilisation system. Both capture and detection antibodies were labeled using Lightning-Link® conjugation kits (ab201796 and ab102890).
Image from Parkash, Om, et al., Diagnostics (Basel), 11(1):33; doi: 10.3390/diagnostics11010033. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
- Schematic Diagram
Supplier Data
Schematic Diagram - Biotinylation Kit / Biotin Conjugation Kit (Fast, Type B) - Lightning-Link® (AB201796)
This illustration demonstrates a general procedure of how the Lightning-Link®(Fast) labeling technology enables the direct labeling of antibodies or proteins.
Simply pipette your antibody or biomolecule of choice into the vial of a lyophilized mixture containing the label of interest and incubate for just 15 minutes. Please see the ab201796 protocol booklet for more details.
Learn more about our Lightning-Link® conjugation kits here
- Conjugation
PubMed
Conjugation - Biotinylation Kit / Biotin Conjugation Kit (Fast, Type B) - Lightning-Link® (AB201796)
Rackus, Darius G., et al used Biotinylation Kit / Biotin Conjugation Kit (Type B) - Lightning-Link® (ab201796) as part of examining digital microfluidics for detection a malaria biomarker. They used the kit to conjugate Biotinylation Kit / Biotin to anti-Plasmodium falciparum LDH antibody for use in pre-concentration by liquid intake by paper (P-CLIP).
DMF immunoassay for PfLDH. Comparison of mean signals obtained by traditional DMF-ELISA (black) and P-CLIP modified DMF-ELISA (grey) for concentrations below the limit of detection (7 ng mL-1) and limit of quantitation (70 ng mL-1) of the traditional DMF-ELISA. Error bars ± 1 std. dev. (n = 3).
Image from Rackus et al., Lab Chip., 17(13):2272-2280; doi: 10.1039/c7lc00440k. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/3.0/
Product details
Biotinylation Kit / Biotin Conjugation Kit ab201796 uses a simple and quick process for biotinylation / biotin labeling of antibodies. It can also be used to conjugate other proteins or peptides. Learn about our antibody labeling kits and their advantages.
To conjugate an antibody to Biotin using this kit:
- add modifier to antibody and incubate for 15 mins
- add quencher and incubate for 5 mins
The conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.
Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to Biotin.
The Type B Biotinylation Kit / Biotin Conjugation Kit is optimized to produce conjugates for assays in which the biotinylated protein is captured by streptavidin immobilized on a surface (e.g., plates, nitrocellulose, magnetic beads etc).
Use the Type A Biotinylation Kit / Biotin Conjugation Kit ab201795 to produce conjugates for assays in which a streptavidin-labeled detection reagent will be used.
Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.
This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link® Rapid Biotin Type B Labeling Kit. 371-0005 is the same as the 100 μg size. 371-0010 is the same as the 3 x 100 ug size. 371-0030 is the same as the 3 x 10 ug size. 371-0015 is the same as the 1 mg size.
Amount and volume of antibody for conjugation to Biotin
| Kit size | Recommended amount of antibody1 |
Maximum amount of antibody |
Maximum antibody volume2 |
| 3 x 10 μg | 3 x 10 μg | 3 x 20 μg | 3 x 10 μL |
| 100 μg | 1 x 100 μg | 1 x 200 μg | 1 x 100 μL |
| 3 x 100 μg | 3 x 100 μg | 3 x 200 μg | 3 x 100 μL |
| 1 x 1 mg | 1 x 1 mg | 1 x 2 mg | 1 x 1 mL |
1 Using the maximum amount of antibody may result in less labelling per antibody.
2 Ideal antibody concentration is 1 mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 2 mg/ml or < 0.5 mg/ml should be diluted /concentrated.
Buffer Requirements for Conjugation
Buffer should be pH 6.5-8.5.
Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.
| 50mM / 0.6% Tris1 | 0.1% BSA2 | 50% glycerol |
| 0.1% sodium azide | PBS | Potassium phosphate |
| Sodium chloride | HEPES | Sucrose |
| Sodium citrate | EDTA | Trehalose |
1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
2 BSA can also interfere with the use of the conjugated antibody in tissue staining.
Incompatible buffer constituents
| Thiomerosal | Proclin | Glycine |
| Arginine | Glutathione | DTT |
If a constituent of the buffer containing your antibody or protein is not listed above, please contact us.
Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.
Storing and handling conjugation kits
Lyophilized Lightning-Link® components are hygroscopic.
Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
- Download websiteProtocolBooklet|zh
Target data
Publications (25)
Recent publications for all applications. Explore the full list and refine your search
The Journal of clinical investigation 135: PubMed40014401
2025
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Journal of pharmaceutical analysis 14:100995 PubMed39850236
2025
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ImmunoHorizons 8:729-739 PubMed39330967
2024
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Immunity 56:2570-2583.e6 PubMed37909039
2023
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The Journal of experimental medicine 220: PubMed37773046
2023
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Journal of translational medicine 21:367 PubMed37286997
2023
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Microsystems & nanoengineering 9:10 PubMed36644334
2023
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Cancer discovery 13:216-243 PubMed36264161
2022
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Nature biotechnology 41:212-221 PubMed36076083
2022
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Physiological genomics 54:296-304 PubMed35759450
2022
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AB201795
Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link®
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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