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Detect successful fluorescent, gold and latex conjugation. Results in 10 minutes.

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Key facts

Assay time

10m

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What's included?

30 Test
Components
Conjugation Checking Kit Positive Control
1 x 1 Vial
Protein A/G Strips
1 x 30 Test
Running Buffer (10X)
2 x 1500 µL

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Detect successful fluorescent, gold and latex conjugation. Results in 10 minutes.

Key facts

Assay time

10m

Storage

Shipped at conditions

Ambient - Cannot Ship with Ice

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

Conjugation Check Kit (ab236554) confirms the conjugation of an antibody in one easy step.

The key component of the kit is a nitrocellulose membrane containing a 'Test line' of immobilized Protein A and Protein G called a "half strip".

Both Protein A and Protein G have a high affinity for the Fc region of a variety of IgG molecules. The "half strips" also contain an absorbent pad to promote and control the flow of sample through the nitrocellulose. This simple qualitative lateral flow assay does not require any specialized or costly equipment.

When the antibody conjugate is run on the Protein A/G strip, it flows along the nitrocellulose, binding to the Protein A and Protein G concentrated at the Test line. When the antibody is successfully conjugated to a colored label, a visible line appears on the strip.

*Please note Conjugate Check Kit is not compatible with the following AMCA, DyLight® 350, Atto390, DyLight® 405, DyLight® 800, DyLight® 755, Alex Fluor® 700 and DyLight® 680.

Benefits:

Quick and easy qualitative test to check success of conjugation

Compatible with antibody conjugates generated using our Lightning-Link® Fluorescent Labelling kits, Gold and Latex Conjugation Kits

Compatible with any other conjugation technology that uses colored labels

No costly equipment required

Small sample volumes required (40 μL)

This product is manufactured by Expedeon, an Abcam company, and was previously called Conjugate Check&Go! Antibody Labeling Confirmation Kit. 4000-0030 is the same as the 30-tests size.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Conjugation - Conjugation Check Kit (ab236554), expandable thumbnail

    Conjugation - Conjugation Check Kit (ab236554)

    Schematic representation of Conjugation Check Kit process.

    When the antibody conjugate is run on the Protein A/G strip, it flows along the nitrocellulose, binding to the Protein A and Protein G concentrated on the Test line. When the antibody is successfully conjugated to a colored label, a visible line appears on the strip.

  • Conjugation - Conjugation Check Kit (ab236554), expandable thumbnail

    Conjugation - Conjugation Check Kit (ab236554)

    Example Conjugation Check Kit results.

    Various concentrations of GOLD conjugated to Rabbit IgG using Gold Conjugation Kit (40 nm, 20 OD) (ab154873). The conjugation was checked using the Conjugation Checking Kit ab236554. The T line containing the protein A/G mixture is immobilised ~13mm from the bottom of the strip.

    OD checked at 530 nm.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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