Cy3® Conjugation Kit (Fast) - Lightning-Link®
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(20 Publications)
- Rapid Conjugation: achieve Cy3® labeling in under 20 minutes with just 30 seconds of hands-on time.
- High Efficiency: ensures 100% antibody recovery, meaning no loss of valuable antibodies.
- Versatility: suitable for conjugating antibodies, proteins, and peptides. Cy3® labeled antibodies can be used immediately in applications such as Western blot (WB), ELISA, and Immunohistochemistry (IHC) without further purification.
- Confidence: cited in over 20 publications.
- FRET
PubMed
FRET - Cy3® Conjugation Kit (Fast) - Lightning-Link® (AB188287)
Mellal, Katia, et al used Cy3® Conjugation Kit (Fast) - Lightning-Link® (ab188287) as part of examining effects of MPE-001 on the CD36/TLR2 interaction. They used the kit to conjugate Cy3® to anti-TLR2 antibody for use in FRET.
Peritoneal MPs were stimulated with 300 ng/ml R-FSL1 in the presence of 10-7M MPE-001 or vehicle. (A) MPE-001 disrupted the interaction between CD36 labeled with Cy5 (red) using the Cy5® Conjugation Kit (Fast) - Lightning-Link® (ab188288) and TLR2 labeled with Cy3® (green) using the Cy3® Conjugation Kit (Fast) - Lightning-Link® (ab188287) as assessed by FRET after 5 min stimulation with R-FSL1. (B) Percentage of energy transfer measured using LSM-700 confocal microscope (Zeiss). One-way ANOVA test with Newman-Keuls post-test for multiple comparison was performed. *P<0.05, **P<0.01 and ***P<0.001 vs R-FSL1. Data are shown as mean ±S.E.M.
Image from Mellal et al., Sci rep., 9(1):12903. doi: 10.1038/s41598-019-49472-8. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
- Schematic Diagram
Supplier Data
Schematic Diagram - Cy3® Conjugation Kit (Fast) - Lightning-Link® (AB188287)
This illustration demonstrates a general procedure of how the Lightning-Link®(Fast) labeling technology enables the direct labeling of antibodies or proteins.
Simply pipette your antibody or biomolecule of choice into the vial of a lyophilized mixture containing the label of interest and incubate for just 15 minutes. Please see the ab188287 protocol booklet for more details.
Learn more about our Lightning-Link® conjugation kits here
- IHC
PubMed
Immunohistochemistry - Cy3® Conjugation Kit (Fast) - Lightning-Link® (AB188287)
Hausmann, Annika, et al used Cy3® Conjugation Kit (Fast) - Lightning-Link® (ab188287) as part of examining flagella expression in mice orally infected with Salmonella Typhimurium (S. Tm). They used the kit to conjugate Cy3® to anti-FliC antibody for use in immunohistochemistry.
Intestinal epithelial cell (IEC)-specific deletion of NAIP1-6 (Naip1-6/IEC; open circles; ntotal S. Tm=776) leads to an increase of flagellated S. Tm at 12 hpi within the cecal mucosa (Naip1-6fl/fl; circles; ntotal S. Tm=238). Quantifications are based on immunofluorescence staining for the flagella subunit FliC. Black bar, median. Statistical analysis, Mann-Whitney-U Test, p-value indicated. Scale bar, 10 μm.
Image from Hausmann et al., Mucosal Immunol., 13(3):530-544; doi: 10.1038/s41385-019-0247-0. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
- Fluorescence Microscopy
PubMed
Fluorescence Microscopy - Cy3® Conjugation Kit (Fast) - Lightning-Link® (AB188287)
Zhang, Fei, Yuan Xie, and Yuhao Bian used Cy3® Conjugation Kit (Fast) - Lightning-Link® (ab188287) as part of examining cardiomyogenic differentiation of adipose-derived stem cells. They used the kit to conjugate TGF-β1 protein to Cy3® for use in confocal microscopy. Confocal microscopy was performed to analyze (A, B) TGF-1 binding to rTGF-1 RI on ADSC cell membranes and to visualize (C, D) the colocalization of rTGF-β1 RI (DMPE-PEG-TGF-β1 RI-FITC) and TGF-1 (Cy3-TGF-1) on the surface of ADSCs at 64 oil immersion (scale bar, 100 μm; *p<0.05, **p<0.01, ***p<0.001)
Image from Zhang et al., Stem Cell Res Ther., 9(1):358; doi: 10.1186/s13287-018-1090-z. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
- Conjugation
PubMed
Conjugation - Cy3® Conjugation Kit (Fast) - Lightning-Link® (AB188287)
Wu, Yue et al used Cy3® Conjugation Kit - Lightning-Link® (ab188287) as part of examining porcine parvovirus (PPV). They used the kit to conjugate Cy3® to standard PPV positive antibody for use in protein chip.
Optimization of the serum dilution. A 2000-fold dilution of serum was determined as a diagnostic concentration of the visible protein chip detection microarray
Image from Wu et al., BMC Vet Res.,16(1):57; doi: 10.1186/s12917-020-02280-z. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/"
Product details
Cy3® Conjugation Kit (Fast) - Lightning-Link® (ab188287) uses a simple and quick process for labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides.
To conjugate an antibody to Cy3® using this kit:
- add modifier to antibody and incubate for 15 min
- add quencher and incubate for 5 min
The Cy3® conjugated antibody can be used immediately in Flow cytometry, WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.
Learn about our antibody labeling kits and their advantages here.
Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our antibody purification and concentration kits.
Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.
Amount and volume of antibody for conjugation to Cy3
| Kit size | Recommended amount of antibody1 |
Maximum amount of antibody |
Maximum antibody volume2 |
| 3 x 10 µg | 3 x 10 µg | 3 x 20 µg | 3 x 10 µL |
| 100 µg | 1 x 100 µg | 1 x 200 µg | 1 x 100 µL |
| 3 x 100 µg | 3 x 100 µg | 3 x 200 µg | 3 x 100 µL |
| 1 mg | 1 x 1 mg | 1 x 2 mg | 1 x 1 mL |
1 Using the maximum amount of antibody may result in less labelling per antibody.
2 Ideal antibody concentration is 1mg/mL. 0.5 - 1 mg/mL can be used if the maximum antibody volume is not exceeded. Antibodies > 2 mg/mL or < 0.5 mg/mL should be diluted /concentrated.
Buffer Requirements for Conjugation
Buffer should be pH 6.5-8.5.
Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.
| 50mM / 0.6% Tris1 | 0.1% BSA2 | 50% glycerol |
| 0.1% sodium azide | PBS | Potassium phosphate |
| Sodium chloride | HEPES | Sucrose |
| Sodium citrate | EDTA | Trehalose |
1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
2 BSA can also interfere with the use of the conjugated antibody in tissue staining.
Incompatible buffer constituents
| Thiomerosal | Proclin | Glycine |
| Arginine | Glutathione | DTT |
If a constituent of the buffer containing your antibody or protein is not listed above, please contact us.
Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.
Storing and handling conjugation kits
Lyophilized Lightning-Link® components are hygroscopic.
Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.
This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link® Rapid Cy3 Labeling Kit. 340-0005 is the same as the 100 µg size. 340-0010 is the same as the 3 x 100 µg size. 340-0030 is the same as the 3 x 10 µg size. 340-0015 is the same as the 1 mg size.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
- Download websiteProtocolBooklet|zh
Target data
Publications (20)
Recent publications for all applications. Explore the full list and refine your search
Alzheimer's & dementia : the journal of the Alzheimer's Association 19:4110-4126 PubMed37249148
2023
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Chemistry (Weinheim an der Bergstrasse, Germany) 29:e202202766 PubMed36279320
2022
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Nature communications 13:4308 PubMed35879297
2022
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Nature communications 13:2540 PubMed35534484
2022
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Development (Cambridge, England) 147: PubMed32321712
2020
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BMC veterinary research 16:57 PubMed32059673
2020
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Mucosal immunology 13:530-544 PubMed31953493
2020
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Scientific reports 9:12903 PubMed31501473
2019
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Stem cell research & therapy 9:358 PubMed30594240
2018
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Immunity, inflammation and disease 6:354-370 PubMed29635889
2018
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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