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Cy3® conjugation / labeling in < 20 mins with 30 secs hands-on time using Cy3® Conjugation Kit (Fast) - Lightning-Link® ab188287. 100% antibody recovery.

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What's included?

3 x 10 µg
Components
Cyanine Dye3
3 x 10 µg
Modifier reagent
1 x 200 µL
Quencher reagent
1 x 200 µL

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Cy3® conjugation / labeling in < 20 mins with 30 secs hands-on time using Cy3® Conjugation Kit (Fast) - Lightning-Link® ab188287. 100% antibody recovery.

Storage

Shipped at conditions

Ambient - Cannot Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Cy3 Conjugation Kit / Cy3 Labeling Kit ab188287 uses a simple and quick process for Cy3 labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides. Learn about our . To conjugate an antibody to Cy3 using this kit: - add modifier to antibody and incubate for 15 mins - add quencher and incubate for 5 mins The Cy3 conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use. The excitation and emmision wavelengths for Cy3® are Ex: 552nm, Em: 565nm. Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid . Use the to learn more about the technology, or about conjugating other proteins and peptides to Cy3. Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.

This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link® Rapid Cy3 Labeling Kit. 340-0005 is the same as the 100 μg size. 340-0010 is the same as the 3 x 100 ug size. 340-0030 is the same as the 3 x 10 ug size. 340-0015 is the same as the 1 mg size.

Amount and volume of antibody for conjugation to Cy3

Kit sizeRecommended
amount of antibody
1
Maximum
amount of antibody
Maximum antibody
volume2
3 x 10 μg3 x 10 μg3 x 20 μg3 x 10 μL
100 μg1 x 100 μg1 x 200 μg1 x 100 μL
3 x 100 μg3 x 100 μg3 x 200 μg3 x 100 μL
1 mg1 x 1 mg1 x 2 mg1 x 1 mL

1 Using the maximum amount of antibody may result in less labelling per antibody.

2 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 2mg/ml or < 0.5 mg/ml should be diluted /concentrated.

Buffer Requirements for Conjugation

Buffer should be pH 6.5-8.5.

Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.

50mM / 0.6% Tris10.1% BSA250% glycerol
0.1% sodium azidePBSPotassium phosphate
Sodium chlorideHEPESSucrose
Sodium citrateEDTATrehalose

1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
2 BSA can also interfere with the use of the conjugated antibody in tissue staining.

Incompatible buffer constituents

ThiomerosalProclinGlycine
ArginineGlutathioneDTT

If a constituent of the buffer containing your antibody or protein is not listed above, please contact us.

Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.

Storing and handling conjugation kits

Lyophilized Lightning-Link® components are hygroscopic.

Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry - Cy3® Conjugation Kit (Fast) - Lightning-Link® (ab188287), expandable thumbnail
    Image from Hausmann et al., Mucosal Immunol., 13(3):530-544; doi: 10.1038/s41385-019-0247-0. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    Immunohistochemistry - Cy3® Conjugation Kit (Fast) - Lightning-Link® (ab188287)

    Hausmann, Annika, et al used Cy3® Conjugation Kit (Fast) - Lightning-Link® (ab188287) as part of examining flagella expression in mice orally infected with Salmonella Typhimurium (S. Tm). They used the kit to conjugate Cy3® to anti-FliC antibody for use in immunohistochemistry.
    Intestinal epithelial cell (IEC)-specific deletion of NAIP1-6 (Naip1-6/IEC; open circles; ntotal S. Tm=776) leads to an increase of flagellated S. Tm at 12 hpi within the cecal mucosa (Naip1-6fl/fl; circles; ntotal S. Tm=238). Quantifications are based on immunofluorescence staining for the flagella subunit FliC. Black bar, median. Statistical analysis, Mann-Whitney-U Test, p-value indicated. Scale bar, 10 μm.

  • Conjugation - Cy3® Conjugation Kit (Fast) - Lightning-Link® (ab188287), expandable thumbnail
    Image from Wu et al., BMC Vet Res.,16(1):57; doi: 10.1186/s12917-020-02280-z. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/"

    Conjugation - Cy3® Conjugation Kit (Fast) - Lightning-Link® (ab188287)

    Wu, Yue et al used Cy3® Conjugation Kit - Lightning-Link® (ab188287) as part of examining porcine parvovirus (PPV). They used the kit to conjugate Cy3® to standard PPV positive antibody for use in protein chip.
    Optimization of the serum dilution. A 2000-fold dilution of serum was determined as a diagnostic concentration of the visible protein chip detection microarray

  • FRET - Cy3® Conjugation Kit (Fast) - Lightning-Link® (ab188287), expandable thumbnail
    Image from Mellal et al., Sci rep., 9(1):12903. doi: 10.1038/s41598-019-49472-8. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    FRET - Cy3® Conjugation Kit (Fast) - Lightning-Link® (ab188287)

    Mellal, Katia, et al used Cy3® Conjugation Kit (Fast) - Lightning-Link® (ab188287) as part of examining effects of MPE-001 on the CD36/TLR2 interaction. They used the kit to conjugate Cy3® to anti-TLR2 antibody for use in FRET.
    Peritoneal MPs were stimulated with 300 ng/ml R-FSL1 in the presence of 10-7M MPE-001 or vehicle. (A) MPE-001 disrupted the interaction between CD36 labeled with Cy5 (red) using the Cy5® Conjugation Kit (Fast) - Lightning-Link® (ab188288) and TLR2 labeled with Cy3® (green) using the Cy3® Conjugation Kit (Fast) - Lightning-Link® (ab188287) as assessed by FRET after 5 min stimulation with R-FSL1. (B) Percentage of energy transfer measured using LSM-700 confocal microscope (Zeiss). One-way ANOVA test with Newman-Keuls post-test for multiple comparison was performed. *P<0.05, **P<0.01 and ***P<0.001 vs R-FSL1. Data are shown as mean ±S.E.M.

  • Fluorescence Microscopy - Cy3® Conjugation Kit (Fast) - Lightning-Link® (ab188287), expandable thumbnail
    Image from Zhang et al., Stem Cell Res Ther., 9(1):358; doi: 10.1186/s13287-018-1090-z. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    Fluorescence Microscopy - Cy3® Conjugation Kit (Fast) - Lightning-Link® (ab188287)

    Zhang, Fei, Yuan Xie, and Yuhao Bian used Cy3® Conjugation Kit (Fast) - Lightning-Link® (ab188287) as part of examining cardiomyogenic differentiation of adipose-derived stem cells. They used the kit to conjugate TGF-β1 protein to Cy3® for use in confocal microscopy. Confocal microscopy was performed to analyze (A, B) TGF-1 binding to rTGF-1 RI on ADSC cell membranes and to visualize (C, D) the colocalization of rTGF-β1 RI (DMPE-PEG-TGF-β1 RI-FITC) and TGF-1 (Cy3-TGF-1) on the surface of ADSCs at 64 oil immersion (scale bar, 100 μm; *p<0.05, **p<0.01, ***p<0.001)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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