DyLight® 488 conjugation / labeling in < 20 mins with 30 secs hands-on time using DyLight® 488 Conjugation Kit (Fast) - Lightning-Link® ab201799. 100% antibody recovery.
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- The Journal of clinical investigation 134:2024Inhibiting the NADase CD38 improves cytomegalovirus-specific CD8+ T cell functionality and metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNils Mülling,Felix M Behr,Graham A Heieis,Kristina Boss,Suzanne van Duikeren,Floortje J van Haften,Iris N Pardieck,Esmé Ti van der Gracht,Ward Vleeshouwers,Tetje C van der Sluis,J Fréderique de Graaf,Dominique Mb Veerkamp,Kees Lmc Franken,Xin Lei,Lukas van de Sand,Sjoerd H van der Burg,Marij Jp Welters,Sebastiaan Heidt,Wesley Huisman,Simon P Jochems,Martin Giera,Oliver Witzke,Aiko Pj de Vries,Andreas Kribben,Bart Everts,Benjamin Wilde,Ramon ArensPubMed 38954588
- Frontiers in endocrinology 14:11601552023Impaired mitochondrial dynamics and removal of the damaged mitochondria in diabetic retinopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKumari Alka,Jay Kumar,Renu A KowluruPubMed 37415667
- Nature communications 14:19102023Tryptase β regulation of joint lubrication and inflammation via proteoglycan-4 in osteoarthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNabangshu Das,Luiz G N de Almeida,Afshin Derakhshani,Daniel Young,Kobra Mehdinejadiani,Paul Salo,Alexander Rezansoff,Gregory D Jay,Christian P Sommerhoff,Tannin A Schmidt,Roman Krawetz,Antoine DufourPubMed 37024468
- Nature communications 13:62752022Single cell transcriptomic profiling of a neuron-astrocyte assembloid tauopathy model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHannah Drew Rickner,Lulu Jiang,Rui Hong,Nicholas K O'Neill,Chromewell A Mojica,Benjamin J Snyder,Lushuang Zhang,Dipan Shaw,Maria Medalla,Benjamin Wolozin,Christine S ChengPubMed 36271092
- International journal of biological sciences 18:6008-60192022Endocytosis of Peptidase Inhibitor SerpinE2 promotes Myocardial Fibrosis through activating ERK1/2 and β-catenin Signaling Pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Li,Li-Fang Lv,Mu-Ge Qi-Li,Rui Yang,Yu-Jing Wang,Shuang-Shuang Chen,Ming-Xiu Zhang,Tian-Yu Li,Tong Yu,Yu-Hong Zhou,Hai-Hai Liang,Hong-Li Shan,Xue-Lian LiPubMed 36439874
- JCI insight 7:2022ARMC5-CUL3 E3 ligase targets full-length SREBF in adrenocortical tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYosuke Okuno,Atsunori Fukuhara,Michio Otsuki,Iichiro ShimomuraPubMed 35862218
- Viruses 14:2022Foot-and-Mouth Disease Virus 3A Hijacks Sar1 and Sec12 for ER Remodeling in a COPII-Independent Manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeng-Wei Lee,Yi-Fan Jiang,Hui-Wen Chang,Ivan-Chen ChengPubMed 35458569
- Methods in molecular biology (Clifton, N.J.) 2258:171-1922020Directed Differentiation of Human Pluripotent Stem Cells for the Generation of High-Order Kidney Organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIdoia Lucía Selfa,Maria Gallo,Nuria Montserrat,Elena GarretaPubMed 33340361
- The Journal of investigative dermatology 141:1416-1427.e122020Isosorbide Di-(Linoleate/Oleate) Stimulates Prodifferentiation Gene Expression to Restore the Epidermal Barrier and Improve Skin Hydration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKrzysztof Bojanowski,William R Swindell,Shyla Cantor,Ratan K ChaudhuriPubMed 33181142
- Translational oncology 13:1008572020Fluorophore-conjugated Helicobacter pylori recombinant membrane protein (HopQ) labels primary colon cancer and metastases in orthotopic mouse models by binding CEA-related cell adhesion molecules.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHannah M Hollandsworth et. al.PubMed 32866936
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DyLight® 488 conjugation / labeling in < 20 mins with 30 secs hands-on time using DyLight® 488 Conjugation Kit (Fast) - Lightning-Link® ab201799. 100% antibody recovery.
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- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
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Notes
DyLight® 488 Conjugation Kit / DyLight® 488 Labeling Kit (ab201799) uses a simple and quick process for DyLight® 488 labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides. Learn about our .To conjugate an antibody to DyLight® 488 using this kit: - add modifier to antibody and incubate for 15 mins - add quencher and incubate for 5 mins The DyLight® 488 conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid . Use the to learn more about the technology, or about conjugating other proteins and peptides to DyLight® 488.Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.
This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link® Rapid DyLight® 488 Labeling Kit. 322-0015 is the same as the 1 mg size. 322-0030 is the same as the 3 x 10 ug size. 322-0010 is the same as the 3 x 100 ug size. 322-0005 is the same as the 100 ug size.
Amount and volume of antibody for conjugation to Dylight ® 488
| Kit size | Recommended amount of antibody1 | Maximum amount of antibody | Maximum antibody volume2 |
| 3 x 10 μg | 3 x 10 μg | 3 x 20 μg | 3 x 10 μL |
| 100 μg | 1 x 100 μg | 1 x 200 μg | 1 x 100 μL |
| 3 x 100 μg | 3 x 100 μg | 3 x 200 μg | 3 x 100 μL |
| 1 mg | 1 x 1 mg | 1 x 2 mg | 1 x 1 mL |
1 Using the maximum amount of antibody may result in less labelling per antibody.
2 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 2mg/ml or < 0.5 mg/ml should be diluted /concentrated.
Buffer Requirements for Conjugation
Buffer should be pH 6.5-8.5.
Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.
| 50mM / 0.6% Tris1 | 0.1% BSA2 | 50% glycerol |
| 0.1% sodium azide | PBS | Potassium phosphate |
| Sodium chloride | HEPES | Sucrose |
| Sodium citrate | EDTA | Trehalose |
1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
2 BSA can also interfere with the use of the conjugated antibody in tissue staining.
Incompatible buffer constituents
| Thiomerosal | Proclin | Glycine |
| Arginine | Glutathione | DTT |
If a constituent of the buffer containing your antibody or protein is not listed above, please contact us.
Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.
Storing and handling conjugation kits
Lyophilized Lightning-Link® components are hygroscopic.
Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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5 product images
Image from Monaco, Sara, et al., Front Cell Neurosci., 12:519; doi: 10.3389/fncel.2018.00519. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/Flow Cytometry - DyLight® 488 Conjugation Kit (Fast) - Lightning-Link® (ab201799)
Monaco, Sara, et al used DyLight® 488 Conjugation Kit (Fast) - Lightning-Link® (ab201799) as part of examining the co-localization of Smoothened and AC3 in primary cilia. They used the kit to conjugate DyLight® 488 to anti-AC3 antibody for use in flow cytometry.
(A) Representative dot plots of untreated (Control) and SAG-treated samples (SAG) from E18 embryos immunostained for AC3-FITC and Smoothened-APC (Smo-APC). (B) Quantification of the percentage of Smo+AC3+ particles. Bar graphs show mean ± SEM, p-values are calculated with Student's t-test. Statistically significant differences are indicated with asterisks (*p < 0.05, **p < 0.01).
Image from Dorfmueller, Simone, et al., Sci rep., 6:21661; doi: 10.1038/srep21661. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/Flow Cytometry - DyLight® 488 Conjugation Kit (Fast) - Lightning-Link® (ab201799)
Dorfmueller, Simone, et al used DyLight® 488 Conjugation Kit (Fast) - Lightning-Link® (ab201799) as part of characterizing antibody-labelled human corneal endothelial cells (hCECs) and stromal fibroblasts. They used the kit to conjugate DyLight® 488 to C1M2-2-B8 scFv or IgG-B8 full-size antibody for use in flow cytometry.
Primary hCECs or stromal fibroblasts were incubated with C1M2-2-B8 scFv or IgG-B8 full-size antibody conjugated to DyLight® 488 (4 μg/ml). (a) The histogram for unlabelled hCECs (black) is overlaid with those for scFv and IgG-B8 labelled cells as indicated. Similarly, the histogram for unlabelled stromal fibroblasts (orange) is overlaid with those for scFv and IgG-B8 labelled fibroblasts. (b) The histograms for C1M2-2-B8 scFv labelled hCECs and stromal fibroblasts were shown on the same graph, as for the histograms for IgG-B8 labelled hCECs and stromal fibroblasts. The peak separation between fibroblasts and hCECs were similar in magnitude with either scFv or IgG labelling.
Image from Mellema et al., PLoS One, 11(12):e0166045; doi: 10.1371/journal.pone.0166045. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/Conjugation - DyLight® 488 Conjugation Kit (Fast) - Lightning-Link® (ab201799)
Mellema, M., et al used DyLight® 488 Conjugation Kit (Fast) - Lightning-Link® (ab201799) as part of examining DyLight® 488-conjugated alendronate binding of calcifying nanoparticles (CNP) generated in vitro. They used the kit to conjugate DyLight® 488 to alendronate (that selectively and specifically bind to calcium-phosphorus surfaces) for use in Nanoparticle Tracking Analysis (NTA®).
CNP in the whole urine samples and in vitro generated CNP suspensions were analyzed using the NanoSight LM10HS-48814TS instrument (Malvern Instruments, Worcestershire, UK) with a 488 nm wavelength laser, fluorescent filter (505 nm long pass), and both temperature control and syringe pump modules. A representative histogram shows averaged sizing and relative abundance data (left; SE in red) and individual analyses from the assessment (right) of this representative sample in triplicate. Note that CNP generated in vitro do not acquire the protein/mucus coating known to occur in vivo and the larger forms retain the ability to bind to alendronate.
Image from Lalo, Ulyana, et al., PLoS Biol., 12(1): e1001747; doi: 10.1371/journal.pbio.1001747. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/Fluorescence Microscopy - DyLight® 488 Conjugation Kit (Fast) - Lightning-Link® (ab201799)
Lalo, Ulyana, et al used DyLight® 488 Conjugation Kit (Fast) - Lightning-Link® (ab201799) as part of examining the colocalization of vesicular ATP transporters with exocytotic organelle markers. They used the kit to conjugate DyLight® 488 to various antibodies for use in multiphoton fluorescent microscopy.
Living, acutely isolated cortical astrocytes were labeled with antibodies to vesicular transporters (VNUT1 and VGLUT1) and synaptic vesicle (SV2A) and lysosomal (LAMP3 and Cathepsin-D) markers. Antibodies were conjugated to fluorescent dyes DyLight488 (green) and DyLight594 (red) prior to astrocyte labeling. (A-D) The representative two-photon fluorescence images (maximal intensity projections of Z-stack) and results of colocalization analysis, carried out using NIH ImageJ 1.43 software. The correlation between green and red fluorescence (images in the right column) is depicted as a product of the relative differences from the mean (PDM) for each pixel; the pseudocolor PDM images were generated as an output of ImageJ analysis routine. Positive values (bright yellow) are indicative for good co-localization of green and red signals, negative values (blue-violet) indicate segregation, and black color shows the lack of correlation. Note the different extent of scale for PDM values in (A-D). All scale bars in (A-D) are 5 μm.
Image from Mellema M et al., PloS One,11(12):e0166045. Fig 4.; doi: 10.1371/journal.pone.0166045. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/Conjugation - DyLight® 488 Conjugation Kit (Fast) - Lightning-Link® (ab201799)
DyLight® 488 Conjugation Kit - Lightning-Link® labeling alendronate compound and osteopontin protein for Nanoparticle Tracking Analysis (NTA®).
Mellema M et al. used ab201799 as part of examining mineralo-organic nanoparticles in feline urine.
They used the kit to conjugate DyLight® 488 to alendronate compound and osteopontin protein for use in Nanoparticle Tracking Analysis (NTA®).
A: Representative histogram showing size distribution and cumulative percentage (left) of submicron particulate matter in healthy feline urine after labeling with DyLight 488 conjugated alendronate. A representative frame from the captured video analyzed by NTA is shown as well (right). Note the strongly preferential binding to primary CNP that lack an outer layer of protein or mucus.
B: Representative histogram showing averaged sizing and relative abundance data (left; SE in red) and individual analyses from the assessment (right) of this representative sample of healthy feline urine after labeling with DyLight 488 conjugated osteopontin. Note the strongly preferential binding to primary naturally-occurring CNP that lack an outer layer of protein or mucus.
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