DyLight® 550 Conjugation Kit (Fast) - Lightning-Link®

• Conjugation

PubMed

Conjugation - DyLight® 550 Conjugation Kit (Fast) - Lightning-Link® (AB201800)

Lucas, Nicolas, et al used DyLight^® 550 Conjugation Kit (Fast) - Lightning-Link^® (ab201800) as part of examining the function of α-melanocyte-stimulating hormone (α-MSH) in Melanocortin 4 receptor (MC4R) signaling in normal and pathological conditions. They used the kit to conjugate DyLight^® 550 to anti-α-MSH IgG for use in confocal microscopy.
Representative images of hMC4R GFP+ HEK 293 cells (green) 30 min after application of DyLight 550^®-labeled (red) α-MSH affinity-purified IgG from eating disorder (anorexia nervosa, n = 9; bulimia nervosa, n = 7; binge eating disorder, n = 7), obese (n = 10), and Ctrl (n = 9) preincubated or not with α-MSH.

Image from Lucas et al., Transl Psychiatry, 9(1):87; doi: 10.1038/s41398-019-0422-9. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

• Schematic Diagram

Supplier Data

Schematic Diagram - DyLight® 550 Conjugation Kit (Fast) - Lightning-Link® (AB201800)

This illustration demonstrates a general procedure of how the Lightning-Link®(Fast) labeling technology enables the direct labeling of antibodies or proteins.

Simply pipette your antibody or biomolecule of choice into the vial of a lyophilized mixture containing the label of interest and incubate for just 15 minutes. Please see the ab201800 protocol booklet for more details.

Learn more about our Lightning-Link® conjugation kits here

• Conjugation

Collaborator

Conjugation - DyLight® 550 Conjugation Kit (Fast) - Lightning-Link® (AB201800)

Staining for mouse-on-mouse immunofluorescence.

Adult mouse testes were fixed with 4% paraformaldehyde. Tissue was embedded in O.C.T. and frozen. 5 micron sections were cut and transferred to slides. Sections were permeabilized with 0.1% Triton X-100 in PBS, and blocked with 3% BSA in 0.1% Triton X-100 + PBS.

Sections were then incubated with either :
(A) Mouse Anti-DDX4 (ab27591) at a 1 : 500 dilution for 1 h at RT. Sections were then washed 3X with 0.1% Triton X-100 in PBS and Donkey Anti-Mouse 555 (ab150110) applied at a 1 : 500 dilution.

(B) Mouse Anti-DDX4 (ab27591) labeled with Dylight 550 Fast Conjugation Kit (ab201800). Sections were incubated with labeled antibody at 1 : 100 dilution for 1 h at RT. Sections were then washed 3X with 0.1% Triton X-100 in PBS and mounted.

DDX4 is shown in red. Phalloidin, which stains F-actin is shown in blue.

Image courtesy of an Abreview submitted by Bryan Niedenberger.

• Conjugation

PubMed

Conjugation - DyLight® 550 Conjugation Kit (Fast) - Lightning-Link® (AB201800)

Soldevila, Ferran, et al used DyLight^® 550 Conjugation Kit (Fast) - Lightning-Link^® (ab201800) as part of examining myeloid cell populations in the porcine palatine tonsil. They used the kit to conjugate DyLight^® 550 to anti-CD14 IgG for use in confocal microscopy.
In situ localization of the CD14+ cells and plasmocytoid dendritic cells in porcine palatine tonsil. CD14+ cells and pDCs were localized by confocal microscopy following ethanol fixation of tonsil slices. The areas assessed included the follicle (F), the interfollicular region (IFA), the crypt (C). The tissue was stained using panel 1 antibodies; white arrow CD14+ cells and yellow arrow pDCs. Images are representative of at least two images from each section, from three different pigs. Objective used : (A) 63× oil immersion. Scale bars as shown.

Image from Soldevila et al., Front Immunol., 9:1800; doi: 10.3389/fimmu.2018.01800. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0