DyLight® 650 Conjugation Kit (Fast) - Lightning-Link®

• Flow Cyt

PubMed

Flow Cytometry - DyLight® 650 Conjugation Kit (Fast) - Lightning-Link® (AB201803)

Saha, Chaitrali, et al used DyLight^® 650 Conjugation Kit (Fast) - Lightning-Link^® (ab201803) as part of examining the ability of monomeric IgA (mIgA) isolated from pooled plasma of healthy donors to modulate human Th17 cells. They used the kit to conjugate DyLight^® 650 to IgA and IgG for use in flow cytometry.
Monomeric IgA (mIgA) binds to CD4+ T cells. (A,B) Representative dot plots and percentage (mean ± SEM, n = 3–9 donors) of binding of DyLight650-conjugated mIgA to CD4+ T cells. Statistical significance as determined by two-tailed Student's t-test is indicated (**P < 0.01). (C,D) Representative dot plots and percentage (mean ± SEM, n = 4) of binding of DyLight650-conjugated mIgA to CD4+ T cells, CD4+CD45RA+ naïve T cells, and CD4+CD45RO+ memory T cells. Statistical significance as determined by one-way ANOVA is indicated (**P < 0.01; ***P < 0.001; ns, not significant).

Image from Saha, Chaitrali, et al., Front Immunol., 8:275. doi: 10.3389/fimmu.2017.00275. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

• Schematic Diagram

Supplier Data

Schematic Diagram - DyLight® 650 Conjugation Kit (Fast) - Lightning-Link® (AB201803)

This illustration demonstrates a general procedure of how the Lightning-Link®(Fast) labeling technology enables the direct labeling of antibodies or proteins.

Simply pipette your antibody or biomolecule of choice into the vial of a lyophilized mixture containing the label of interest and incubate for just 15 minutes. Please see the ab201803 protocol booklet for more details.

Learn more about our Lightning-Link® conjugation kits here

• Fluorescence Microscopy

PubMed

Fluorescence Microscopy - DyLight® 650 Conjugation Kit (Fast) - Lightning-Link® (AB201803)

Fluorescence Microscopy - DyLight Lightning-Link.

Jiang, Peizhou, et al used DyLight^® 650 Conjugation Kit - Lightning-Link^® (ab201803) as part of examining α-synuclein aggregates. They used the kit to conjugate DyLight^® 650 to α-synuclein protein aggregates for use in confocal microscopy.
Membrane penetration- and endocytosis-mediated αS seeding contribute to formation of endo-lysosome-free and endo-lysosome-associated αS inclusions. (a) H4/V1S-SV2/LAMP1-eCFP cells were respectively treated with DyLight® 650 labeled αS seeds (Agg. αS), Dynasore, and Dynasore & Agg. αS for 2 days followed by imaging under confocal microscopy. The concentration for Agg. αS is 0.5 μg/ ml, and Dynasore 20 μM. Yellow and white arrows denote αS seeds and induced LAMP1-free (yellow), LAMP1-positive (white) αS inclusion, respectively. Scale bar : 5 μm. (b) Bar graphs show the comparisons among Agg. αS, Dynasore, and Dynasore & Agg. αS treated cells in the ratio of cells with inclusions to total cells, inclusions to total seeds, LAMP1-positive seeds to total seeds, LAMP1-negative and LAMP1-positive inclusions to total inclusions, respectively. Error bars represent standard error of the mean (N.S = non-significant; **p < 0.01, comparing subsets linked by line, n = 3).

Image from Jiang, Peizhou, et al., Scientific reports 7.1 (2017): 1-13. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/