The Abcam Gold Conjugation Kit (ab154873) offers:
- Rapid Conjugation: allows for gold conjugation or labeling in less than 4 hours with only 30 seconds of hands-on time.
- High Recovery: It ensures 100% antibody recovery, meaning no loss of valuable antibodies.
- Stability and Efficiency: The gold nanoparticles have a protective surface coat that provides unrivaled colloidal stability, and the conjugation reaction is initiated simply by reconstituting the freeze-dried nanoparticle
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- Tropical medicine and infectious disease 7:2022Non-Invasive Detection of SARS-CoV-2 Antigen in Saliva versus Nasopharyngeal Swabs Using Nanobodies Conjugated Gold Nanoparticles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManal Kamel,Sara Maher,Hanan El-Baz,Faten Salah,Omar Sayyouh,Zeinab DemerdashPubMed 35736981
- Biosensors 12:2022Development of a Self-Viscosity and Temperature-Compensated Technique for Highly Stable and Highly Sensitive Bead-Based Diffusometry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei-Long Chen,Han-Sheng ChuangPubMed 35735510
- PLoS neglected tropical diseases 16:e00103112022Monoclonal antibody pairs against SARS-CoV-2 for rapid antigen test development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNol Salcedo,Ankita Reddy,Adam R Gomez,Irene Bosch,Bobby Brooke HerreraPubMed 35358198
- Current research in biotechnology 3:288-2992021Highly portable quantitative screening test for prostate-specific antigen at point of care.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBalaji Srinivasan,David M Nanus,David Erickson,Saurabh MehtaPubMed 35083431
- Scientific reports 11:78802021Carbohydrate binding module-fused antibodies improve the performance of cellulose-based lateral flow immunoassays.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdrian Elter,Tina Bock,Dieter Spiehl,Giulio Russo,Steffen C Hinz,Sebastian Bitsch,Eva Baum,Markus Langhans,Tobias Meckel,Edgar Dörsam,Harald Kolmar,Gerhard SchwallPubMed 33846482
- Biosensors & bioelectronics 171:1127152020A novel rapid detection for SARS-CoV-2 spike 1 antigens using human angiotensin converting enzyme 2 (ACE2).Applications:Unspecified applicationReactive species:Unspecified reactive speciesJong-Hwan Lee,Minsuk Choi,Yujin Jung,Sung Kyun Lee,Chang-Seop Lee,Jung Kim,Jongwoo Kim,Nam Hoon Kim,Bum-Tae Kim,Hong Gi KimPubMed 33099241
- Biosensors 10:2020Colorimetric Diagnostic Capillary Enabled by Size Sieving in a Porous Hydrogel.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohn Mello Camille C Guzman et. al.PubMed 32977557
- EBioMedicine 42:504-5102019Rapid diagnostics for point-of-care quantification of soluble transferrin receptor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBalaji Srinivasan,Julia L Finkelstein,Dakota O'Dell,David Erickson,Saurabh MehtaPubMed 30885726
- Scientific reports 7:141422017A Quantitative Point-of-Need Assay for the Assessment of Vitamin D Deficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS Vemulapati et. al.PubMed 29074843
- Biosensors & bioelectronics 101:75-832017Enhanced diffusometric immunosensing with grafted gold nanoparticles for detection of diabetic retinopathy biomarker tumor necrosis factor-α.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHan-Sheng Chuang,Yu-Ju Chen,Hui-Pin ChengPubMed 29040917
What's included?
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The Abcam Gold Conjugation Kit (ab154873) offers:
- Rapid Conjugation: allows for gold conjugation or labeling in less than 4 hours with only 30 seconds of hands-on time.
- High Recovery: It ensures 100% antibody recovery, meaning no loss of valuable antibodies.
- Stability and Efficiency: The gold nanoparticles have a protective surface coat that provides unrivaled colloidal stability, and the conjugation reaction is initiated simply by reconstituting the freeze-dried nanoparticle
Storage
- Shipped at conditions
- Ambient - Cannot Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's Gold Conjugation Kit allows antibodies or proteins to be covalently attached to ultra-stable Gold nanoparticles at very high OD quickly and easily. The nanoparticles in the Abcam's Gold Conjugation Kit have a protective surface coat that can withstand the most extreme conditions (e.g. 2.5M NaOH at 70°C for > 1 hour). The hands-on time for the Abcam Gold conjugation procedure is around 2 minutes and the conjugate is ready to use within 20 minutes. The researcher simply pipettes the biomolecule into a vial containing the nanoparticles of the Gold Conjugation Kit.
The nanoparticles in this kit are supplied as a freeze-dried mixture. The conjugation reaction is initiated simply by reconstituting the freeze-dried nanoparticles with the antibody, which becomes attached (through lysine residues) to the surface of the nanoparticles.
The resulting covalent conjugates are more stable than those prepared by passive adsorption methods. Moreover, unlike passive methods, the coating process is independent of the isoelectric point of the antibody, avoiding the need for extensive trials at different pH values. All antibodies can be labelled at a single pH.
Learn more about buffer compatibility, protein/secondary antibody conjugation and labeling chemistry in our FAQs.
Benefits
Easy and rapid conjugation – only 2 minutes hands-on time and 100% recovery of materials
Site-specific labelling – Antigen binding sites of antibodies are free to bind the target molecule
Proprietary surface coating prevents metal-protein interactions, and enables covalent attachment to the Gold – Stable conjugates formed
Fully scalable – Easy transfer from R&D to manufacturing
Uniform spherical shape and narrow size distribution – Consistent high quality and excellent batch-to-batch reproducibility
Buffer requirements:
The biomolecule to be conjugated should ideally be in 10 mM amine-free buffer (e.g. MES, MOPS, HEPES), pH range 6.5 to 8.5. Sugars have no effect on conjugation efficiency. For incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits for Nanoparticles. To learn more about incompatible buffers, please refer to the protocol booklet.
This product is manufactured by Expedeon, an Abcam company, and was previously called 40nm InnovaCoat® GOLD. 230-0010 is the same as the 10 x 1 μg size. 230-0015 is the same as the 1 x 10 μg size. 230-0005 is the same as the 3 x 1 μg size.
The 3 and 10 Test Conjugation Kits are designed to label 12 μl of antibody per vial.
The 1 Test Conjugation Kit is designed to label 120 μl of antibody per vial.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Conjugation - Gold Conjugation Kit (40nm, 20 OD) (ab154873)
GOLD Conjugation Kit (40nm, 20 OD).
Please see the protocol booklet for a detailed method.
Image from Guzman et al., Biosensors (Basel), 10(10):130; doi: 10.3390/bios10100130.Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/Conjugation - Gold Conjugation Kit (40nm, 20 OD) (ab154873)
Guzman, John Mello Camille C., Sheng-Min Hsu, and Han-Sheng Chuang used Gold Conjugation Kit (40nm, 20 OD) (ab154873) as part of developping a colorimetric diagnostic capillary device for the rapid detection of biomarkers. They used the kit to conjugate Gold to probe rabbit polyclonal anti-LCN antibody for use in the device.
(a) Illustrations for preparing sandwiched immunocomplex and detection process. (b) Sandwiched immunocomplex detection process. (c-g) Quantitative assessment of the detection platform: (c) grayscale images of diagnostic tool inside the detection box; (d) calibration of the grayscale intensity of sandwiched immunocomplex with respect to different lipocalin-1 (LCN-1) concentrations ranging from 100 pg/mL to 1 μg/mL; (e) binding specificity of the capture and probe particles in the presence of different antigens; (f) binding stability of sandwiched immunocomplex with respect to different times; (g) reliability of the detection platform. Comparison of LCN-1 concentration detection results between the proposed diagnostic tool and spectrophotometer. The symbols "*", "**", "***", "ns" denote p < 0.05, p < 0.01, p < 0.001, and p > 0.05, respectively, under student's t test (n = 5). The error bars represent standard deviation.
Image from Lee et al., Scientific reports 6 (2016): 28237. Sci Rep., 6:28237; doi: 10.1038/srep28237. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/Lateral flow assay - Gold Conjugation Kit (40nm, 20 OD) (ab154873)
Lee, Seoho, et al used Gold Conjugation Kit (40nm, 20 OD) (ab154873) as part of examining low-cost point-of-care quantification of vitamin B12 concentrations. They used the kit to conjugate Gold to anti-vitamin B12 IgG for use in competitive lateral flow assay.
(A) Colorimetric variation of the test and control line regions on the silver enhanced B12 lateral flow test strip at different known concentrations of standard vitamin B12 samples (B) image processing algorithm used by the NutriPhone platform (C) Test and control line signals detected by the NutriPhone app as local intensity minima for 0 and 369 pmol/L of B12 standard samples (D) Calibration curve showing the T/C ratios of the colorimetric signals at different standard B12 concentrations.
Image from Vemulapati et al., Sci Rep., 7(1):14142; doi: 10.1038/s41598-017-13044-5. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/Lateral flow assay - Gold Conjugation Kit (40nm, 20 OD) (ab154873)
Vemulapati, S., et al used Gold Conjugation Kit (40nm, 20 OD) (ab154873) as part of examining vitamin D3 Deficiency. They used the kit to conjugate Gold to monoclonal anti-25(OH)D3 IgG antibody for use in lateral flow assay.
Vitamin D3 Lateral Flow Assay. (A) Image and schematic of the 25(OH)D3 strip architecture and components. (B) Image and intensity plot of a participant with low Vitamin D3 and high T/C ratio (C) Image and intensity plot of a participant with healthy levels of 25(OH)D3 and low T/C ratio.
Functional Studies - Gold Conjugation Kit (40nm, 20 OD) (ab154873)
Lateral flow data – Gold Conjugation Kit vs traditional gold nanoparticle passive absorption techniques.
Antibody conjugation using the Gold Conjugation Kit vs traditional gold nanoparticle passive absorption techniques with uncoated gold nanoparticles, showing both enhanced signal intensity and improved specificity. 40 nm Gold particles were labeled with anti-IgA antibody and used to measure IgA concentration in a lateral flow inhibition assay, with IgA bound to a lateral flow strip.
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