HRP Antibody Conjugation Check Kit
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- Compatible with HRP Conjugation Kit - Lightning-Link® (ab102890)
- Small volumes of conjugate required (40 µL)
- FuncS
Supplier Data
Functional Studies - HRP Antibody Conjugation Check Kit (AB236555)
HRP Antibody Conjugation Check Kit.
Goat anti-rabbit IgG was conjugated with Lightning-Link® HRP and run at different concentrations on Protein A / G strips. After the addition of HRP Detection Solution, a blue signal developed at the T line. The range of detection to obtain a clear, strong result is from 0.5ng/mL to 10ng/mL of antibody conjugate.
- FuncS
Supplier Data
Functional Studies - HRP Antibody Conjugation Check Kit (AB236555)
HRP Antibody Conjugation Check Kit.
IgG antibodies from mouse, rabbit and goat were conjugated with Lightning-Link® HRP and run at different concentrations on Protein A / G strips. After the addition of HRP Detection Solution, a blue signal developed at the T line. There is a strong visible signal for the IgG of all three species within the suggested range (0.5ng / ml to 10ng / ml of antibody–conjugate).
- Schematic Diagram
Supplier Data
Schematic Diagram - HRP Antibody Conjugation Check Kit (AB236555)
How The Kit Works.
The nitrocellulose membrane contains a Test line of immobilized Protein A and Protein G called a "half strip". Both Protein A and Protein G have a high affinity for the Fc region of a variety of IgG molecules (see table "Protein A and Protein G affinity for immunoglobulins"). The half strips also contain an absorbent pad to promote and control the flow of sample through the nitrocellulose. The HRP-antibody conjugate is run on the Protein A/G Strip. The conjugate binds the Protein A and Protein G concentrated on the test line. After the addition of the HRP detection solution, a visible line appears on the strip.
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Product details
HRP Antibody Conjugation Check Kit (ab236555) is a quick immunochromatography test that allows to confirm the successful conjugation of Horseradish Peroxidase (HRP) to an antibody without the need for any specialized or costly equipment.
The key component of the kit is a nitrocellulose membrane containing a Test line of immobilized Protein A and Protein G called a half strip. Both Protein A and Protein G have a high affinity for the Fc region of a variety of IgG molecules. The half strips also contain an absorbent pad to promote and control the flow of sample through the nitrocellulose. The HRP-antibody conjugate is run on the Protein A/G Strip. The conjugate binds the Protein A and Protein G concentrated on the Test line. After the addition of the HRP detection solution, a visible line appears on the strip.
Benefits:
Quick and easy qualitative test to check success of conjugation
Sensitivity: the range of detection is between 0.5 ng/mL to 10 ng/mL.
Compatible with antibody conjugates generated with Lightning-Link® HRP conjugation kit (ab102890) or any other HRP conjugation technologies available commercially
No costly equipment required
Small sample volumes required (40 μL)
This product is manufactured by Expedeon, an Abcam company. It was previously called HRP Check&Go! Antibody Labeling Confirmation Kit. Expedeon product code 4002-0030 is the same as the 30-test size of this product.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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