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HRP conjugation / labeling in < 4 hrs with 30 secs hands-on time using HRP Antibody Conjugation Check Kit ab236555. 100% antibody recovery.

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Images

Schematic Diagram - HRP Antibody Conjugation Check Kit (AB236555), expandable thumbnail
  • Functional Studies - HRP Antibody Conjugation Check Kit (AB236555), expandable thumbnail
  • Functional Studies - HRP Antibody Conjugation Check Kit (AB236555), expandable thumbnail

What's included?

30 Test
Components
GAR-HRP (lyophilized)
1 x 1 Vial
HRP detection solution
1 x 1500 µL
Protein A/G Strips
1 x 30 Test
Running Buffer (10X)
3 x 1500 µL

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HRP conjugation / labeling in < 4 hrs with 30 secs hands-on time using HRP Antibody Conjugation Check Kit ab236555. 100% antibody recovery.

Storage

Shipped at conditions
Ambient - Cannot Ship with Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols

Notes

HRP Antibody Conjugation Check Kit (ab236555) is a quick immunochromatography test that allows to confirm the successful conjugation of Horseradish Peroxidase (HRP) to an antibody without the need for any specialized or costly equipment.

The key component of the kit is a nitrocellulose membrane containing a Test line of immobilized Protein A and Protein G called a half strip. Both Protein A and Protein G have a high affinity for the Fc region of a variety of IgG molecules. The half strips also contain an absorbent pad to promote and control the flow of sample through the nitrocellulose. The HRP-antibody conjugate is run on the Protein A/G Strip. The conjugate binds the Protein A and Protein G concentrated on the Test line. After the addition of the HRP detection solution, a visible line appears on the strip.

Benefits:

Quick and easy qualitative test to check success of conjugation

Sensitivity: the range of detection is between 0.5 ng/mL to 10 ng/mL.

Compatible with antibody conjugates generated with Lightning-Link® HRP conjugation kit (HRP Conjugation Kit - Lightning-Link® ab102890) or any other HRP conjugation technologies available commercially

No costly equipment required

Small sample volumes required (40 μL)

This product is manufactured by Expedeon, an Abcam company. It was previously called HRP Check&Go! Antibody Labeling Confirmation Kit. Expedeon product code 4002-0030 is the same as the 30-test size of this product.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Schematic Diagram - HRP Antibody Conjugation Check Kit (ab236555), expandable thumbnail

    Schematic Diagram - HRP Antibody Conjugation Check Kit (ab236555)

    How The Kit Works.

    The nitrocellulose membrane contains a Test line of immobilized Protein A and Protein G called a "half strip". Both Protein A and Protein G have a high affinity for the Fc region of a variety of IgG molecules (see table "Protein A and Protein G affinity for immunoglobulins"). The half strips also contain an absorbent pad to promote and control the flow of sample through the nitrocellulose. The HRP-antibody conjugate is run on the Protein A/G Strip. The conjugate binds the Protein A and Protein G concentrated on the test line. After the addition of the HRP detection solution, a visible line appears on the strip.

  • Functional Studies - HRP Antibody Conjugation Check Kit (ab236555), expandable thumbnail

    Functional Studies - HRP Antibody Conjugation Check Kit (ab236555)

    HRP Antibody Conjugation Check Kit.

    Goat anti-rabbit IgG was conjugated with Lightning-Link® HRP and run at different concentrations on Protein A / G strips. After the addition of HRP Detection Solution, a blue signal developed at the T line. The range of detection to obtain a clear, strong result is from 0.5ng/mL to 10ng/mL of antibody conjugate.

  • Functional Studies - HRP Antibody Conjugation Check Kit (ab236555), expandable thumbnail

    Functional Studies - HRP Antibody Conjugation Check Kit (ab236555)

    HRP Antibody Conjugation Check Kit.

    IgG antibodies from mouse, rabbit and goat were conjugated with Lightning-Link® HRP and run at different concentrations on Protein A / G strips. After the addition of HRP Detection Solution, a blue signal developed at the T line. There is a strong visible signal for the IgG of all three species within the suggested range (0.5ng / ml to 10ng / ml of antibody–conjugate).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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