HRP Antibody Conjugation Check Kit

3 product images

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  Schematic Diagram - HRP Antibody Conjugation Check Kit (ab236555)

  How The Kit Works.

  The nitrocellulose membrane contains a Test line of immobilized Protein A and Protein G called a "half strip". Both Protein A and Protein G have a high affinity for the Fc region of a variety of IgG molecules (see table "Protein A and Protein G affinity for immunoglobulins"). The half strips also contain an absorbent pad to promote and control the flow of sample through the nitrocellulose. The HRP-antibody conjugate is run on the Protein A/G Strip. The conjugate binds the Protein A and Protein G concentrated on the test line. After the addition of the HRP detection solution, a visible line appears on the strip.

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  Functional Studies - HRP Antibody Conjugation Check Kit (ab236555)

  HRP Antibody Conjugation Check Kit.

  Goat anti-rabbit IgG was conjugated with Lightning-Link® HRP and run at different concentrations on Protein A / G strips. After the addition of HRP Detection Solution, a blue signal developed at the T line. The range of detection to obtain a clear, strong result is from 0.5ng/mL to 10ng/mL of antibody conjugate.

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  Functional Studies - HRP Antibody Conjugation Check Kit (ab236555)

  HRP Antibody Conjugation Check Kit.

  IgG antibodies from mouse, rabbit and goat were conjugated with Lightning-Link® HRP and run at different concentrations on Protein A / G strips. After the addition of HRP Detection Solution, a blue signal developed at the T line. There is a strong visible signal for the IgG of all three species within the suggested range (0.5ng / ml to 10ng / ml of antibody–conjugate).