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AB312810

iFluor® Red 700 Conjugation Kit

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iFluor ® Red 700 Conjugation Kit (ab312810) offers several standout features:

- Rapid Conjugation: achieve iFluor® Red 700 labeling in under 2 hours with minimal hands-on time.
- Labels up to 100 µg antibody.
- Versatility: suitable for conjugating antibodies and proteins. iFluor® Red 700 -labeled antibodies can be used immediately in applications such as Flow cytometry, ICC/IF and other fluorescence imaging assays without further purification.
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Flow Cytometry - iFluor® Red 700 Conjugation Kit (AB312810)
  • Flow Cyt

Supplier Data

Flow Cytometry - iFluor® Red 700 Conjugation Kit (AB312810)

Flow cytometric analysis of HL-60 cells incubated with (red) or without (green) anti-human HLA-ABC (W6/32 mAb). Cells were then incubated with goat anti-mouse IgG labeled using the iFluor® 700 Antibody Labeling Kit (ab312810). The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in the APC channel.

Key facts

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Product details

iFluor® Red 700 Conjugation Kit provides a convenient method for labelling small amounts of monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the Red Laser-excitable iFluor® Red 700. This dye is optimized to have minimal fluorescence quenching effect on proteins and nucleic acids, with a fluorescence maxima (Ex/Em) of 693/713 nm respectively. These characteristics make it an excellent replacement for Alexa Fluor 700. iFluor® Red 700 is a stable dye and shows good reactivity and selectivity with protein amino groups.

This conjugation kit has all the essential components to perform two separate labelling reactions with no column purification needed. Each of the two vials of iFluor® Red 700 provided in the kit is optimized for labelling ~50 μg antibody

Impure antibodies or antibodies stabilized with bovine serum albumin (BSA) or gelatin will not be labelled well, Abcam offers a range of purification kits

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What's included?

{ "values": { "100g": { "sellingSize": "100 µg", "publicAssetCode":"ab312810-100ug", "assetComponentDetails": [ { "size":"1 x 2 Vial", "name":"iFluor™ 700", "number":"AB312810-CMP01", "productcode":"" }, { "size":"1 x 20 µL", "name":"Reaction Buffer", "number":"AB312810-CMP02", "productcode":"" }, { "size":"1 x 20 µL", "name":"TQ™-Dyed Quench Buffer", "number":"AB312810-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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