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iFluor® Red 750 conjugation / labeling in < 4 hrs with 30 secs hands-on time using iFluor® Red 750 Conjugation Kit ab312811. 100% antibody recovery.

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Images

Flow Cytometry - iFluor® Red 750 Conjugation Kit (AB312811), expandable thumbnail

What's included?

100 µg
Components
Reaction Buffer
1 x 20 µL
TQ™-Dyed Quench Buffer
1 x 20 µL
iFluor™ 750
1 x 2 Vial

iFluor® Red 750 conjugation / labeling in < 4 hrs with 30 secs hands-on time using iFluor® Red 750 Conjugation Kit ab312811. 100% antibody recovery.

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

iFluor® Red 750 Conjugation Kit provides a convenient method for labelling small amounts of monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the Red Laser-excitable iFluor® Red 750. This dye is optimized to have minimal fluorescence quenching effect on proteins and nucleic acids, with a fluorescence maxima (Ex/Em) of 753/779 nm respectively. Comparison studies indicate that iFluor® 750 is significantly brighter than the corresponding Cy7® and Alexa Fluor® 750, These characteristics make it an excellent replacement for these dyes. iFluor® Red 750 is a stable dye and shows good reactivity and selectivity with protein amino groups.

This conjugation kit has all the essential components to perform two separate labelling reactions with no column purification needed. Each of the two vials of iFluor® Red 750 provided in the kit is optimized for labelling ~50 μg antibody

Impure antibodies or antibodies stabilized with bovine serum albumin (BSA) or gelatin will not be labelled well, Abcam offers a range of purification kits

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Flow Cytometry - iFluor® Red 750 Conjugation Kit (ab312811), expandable thumbnail

    Flow Cytometry - iFluor® Red 750 Conjugation Kit (ab312811)

    Flow cytometric analysis of HL-60 cells incubated with (red) or without (green) anti-human HLA-ABC (W6/32 mAb). Cells were then incubated with goat anti-mouse IgG labeled using the iFluor® 750 Antibody Labeling Kit (ab312811). The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in the APC-Cy7 channel.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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