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AB312812

iFluor® Red 750 Conjugation Kit (BSA compatible)

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iFluor® Red 750 Conjugation Kit (BSA compatible) (ab312812) offers several standout features:

- Rapid Conjugation: achieve iFluor® Red 750 labeling in under 2 hours with minimal hands-on time.
- Compatible with antibodies containing 0.1% - 0.5% BSA.
- Labels up to 100 µg antibody.
- Versatility: suitable for conjugating antibodies and proteins. iFluor® Red 750-labeled antibodies can be used immediately in applications such as Flow cytometry, ICC/IF and other fluorescence imaging assays without further purification.
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Flow Cytometry - iFluor® Red 750 Conjugation Kit (BSA compatible) (AB312812)
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Supplier Data

Flow Cytometry - iFluor® Red 750 Conjugation Kit (BSA compatible) (AB312812)

Flow cytometric analysis of HL-60 cells were incubated with (red) or without (green) anti-human HLA-ABC (W6/32 mAb). Cells were then incubated with goat anti-mouse IgG labeled using the iFluor® 750 Antibody Labeling Kit (BSA compatible) (ab312812). The fluorescence signal was monitored using an ACEA NovoCyte flow cytometer in the APC-Cy7 channel.

Key facts

Detection method

Fluorescent

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Product details

iFluor® Red 750 Conjugation Kit (BSA compatible) provides a convenient method for labelling small amounts of monoclonal, polyclonal antibodies or other proteins (>10 kDa) stabilised with BSA with the Red Laser-excitable iFluor® Red 750. This dye is optimized to have minimal fluorescence quenching effect on proteins and nucleic acids, with a fluorescence maxima (Ex/Em) of 759/777 nm nm respectively. Comparison studies indicate that iFluor® 750 is significantly brighter than the corresponding Cy7® and Alexa Fluor® 750, These characteristics make it an excellent replacement for these dyes. iFluor® Red 750 is a stable dye and shows good reactivity and selectivity with protein amino groups.

This conjugation kit has all the essential components to perform two separate labelling reactions with no column purification needed. Each of the two vials of iFluor® Red 750 provided in the kit is optimized for labelling ~50 μg antibody.

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What's included?

{ "values": { "100g": { "sellingSize": "100 µg", "publicAssetCode":"ab312812-100ug", "assetComponentDetails": [ { "size":"1 x 2 Vial", "name":"Preactivated iFluor™ 750", "number":"AB312812-CMP01", "productcode":"" }, { "size":"1 x 20 µL", "name":"Reaction Buffer", "number":"AB312812-CMP02", "productcode":"" }, { "size":"1 x 20 µL", "name":"TQ™-Dyed Quench Buffer", "number":"AB312812-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

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websiteProtocolBooklet
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Product promise

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