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iFluor™ 350 conjugation / labeling in < 4 hrs with 30 secs hands-on time using iFluor® Ultraviolet 350 Conjugation Kit ab312809. 100% antibody recovery.

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Images

Immunocytochemistry/ Immunofluorescence - iFluor® Ultraviolet 350 Conjugation Kit (AB312809), expandable thumbnail

What's included?

100 µg
Components
Reaction Buffer
1 x 20 µL
TQ™-Dyed Quench Buffer
1 x 20 µL
iFluor™ 350
1 x 2 Vial

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iFluor™ 350 conjugation / labeling in < 4 hrs with 30 secs hands-on time using iFluor® Ultraviolet 350 Conjugation Kit ab312809. 100% antibody recovery.

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

iFluor® Ultraviolet 350 Conjugation Kit provides a convenient method for labelling small amounts of monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the Ultraviolet Laser-excitable iFluor® Ultraviolet 350. This dye is optimized to have minimal fluorescence quenching effect on proteins and nucleic acids, with a fluorescence maxima (Ex/Em) of 350/440 nm respectively. : iFluor® Ultraviolet 350 is a stable dye and shows good reactivity and selectivity with protein amino groups.

This conjugation kit has all the essential components to perform two separate labelling reactions with no column purification needed. Each of the two vials of iFluor® Ultraviolet 350 provided in the kit is optimized for labelling ~50 μg antibody

Impure antibodies or antibodies stabilized with bovine serum albumin (BSA) or gelatin will not be labelled well, Abcam offers a range of purification kits

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Immunocytochemistry/ Immunofluorescence - iFluor® Ultraviolet 350 Conjugation Kit (ab312809), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - iFluor® Ultraviolet 350 Conjugation Kit (ab312809)

    Immunofluorescent analysis of HeLa cells labeling tubulin with mouse anti-tubulin followed by a goat anti-mouse IgG labeled using the iFluor® Ultraviolet  350 Conjugation Kit (ab312809).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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