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AB312809

iFluor® Ultraviolet 350 Conjugation Kit

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iFluor ® Ultraviolet 350 Conjugation Kit (ab312809) offers several standout features:

- Rapid Conjugation: achieve iFluor® Ultraviolet 350 labeling in under 2 hours with minimal hands-on time.
- Labels up to 100 µg antibody.
- Versatility: suitable for conjugating antibodies and proteins. iFluor® Ultraviolet 350-labeled antibodies can be used immediately in applications such as Flow cytometry, ICC/IF and other fluorescence imaging assays without further purification.
1 Images
Immunocytochemistry/ Immunofluorescence - iFluor® Ultraviolet 350 Conjugation Kit (AB312809)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - iFluor® Ultraviolet 350 Conjugation Kit (AB312809)

Immunofluorescent analysis of HeLa cells labeling tubulin with mouse anti-tubulin followed by a goat anti-mouse IgG labeled using the iFluor® Ultraviolet  350 Conjugation Kit (ab312809).

Key facts

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Product details

iFluor® Ultraviolet 350 Conjugation Kit provides a convenient method for labelling small amounts of monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the Ultraviolet Laser-excitable iFluor® Ultraviolet 350. This dye is optimized to have minimal fluorescence quenching effect on proteins and nucleic acids, with a fluorescence maxima (Ex/Em) of 350/440 nm respectively. : iFluor® Ultraviolet 350 is a stable dye and shows good reactivity and selectivity with protein amino groups.

This conjugation kit has all the essential components to perform two separate labelling reactions with no column purification needed. Each of the two vials of iFluor® Ultraviolet 350 provided in the kit is optimized for labelling ~50 μg antibody

Impure antibodies or antibodies stabilized with bovine serum albumin (BSA) or gelatin will not be labelled well, Abcam offers a range of purification kits

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What's included?

{ "values": { "100g": { "sellingSize": "100 µg", "publicAssetCode":"ab312809-100ug", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"iFluor™ 350", "number":"AB312809-CMP01", "productcode":"" }, { "size":"1 x 20 µL", "name":"Reaction Buffer", "number":"AB312809-CMP02", "productcode":"" }, { "size":"1 x 20 µL", "name":"TQ™-Dyed Quench Buffer", "number":"AB312809-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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