Latex Conjugation Kit - 400nm Blue

• FuncS

Supplier Data

Functional Studies - Latex Conjugation Kit - 400nm Blue (AB269892)

Lateral Flow Assay of our specially treated latex beads conjugated to a monoclonal anti-CRP antibody (mAb1) titrated against CRP. 2ng/mL CRP can easily be detected.

• Flow Cyt

PubMed

Flow Cytometry - Latex Conjugation Kit - 400nm Blue (AB269892)

Latex Conjugation Kit – 400nm Blue.

Rutkowska, Aleksandra, et al used Latex Conjugation Kit – 400nm Blue (ab269892) as part of developing a novel method for rapid and quantitative detection of bisphenol A (BPA) in urine. They used the kit to conjugate Latex to polyclonal anti-Bisphenol A antibodies for use in lateral flow assay.
(A) A scheme of using lateral flow assay for detection of BPA in urine samples. (B) Determination of BPA concentration in urine samples by lateral flow strips and estimation of different levels of individual exposure. (C) Examples of results of the lateral flow assay for differ-ent BPA concentrations in the urine samples, with results validated by LC-MS/MS.

Image from Rutkowska et al., Acta Biochim., 67(3):409-415; doi: 10.18388/abp.2020_5368. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by-sa/4.0/

• FuncS

Supplier Data

Functional Studies - Latex Conjugation Kit - 400nm Blue (AB269892)

Lateral Flow Assay of our specially treated latex conjugated to the monoclonal anti-CRP antibody (mAb2) titrated against CRP in buffer or CRP spiked CRP depleted serum (100 % serum). The latex behaves similarly in serum and buffer, and no aggregation or nonspecific binding is seen.

• LFA

PubMed

Lateral flow assay - Latex Conjugation Kit - 400nm Blue (AB269892)

Kim, Jinsu, et al used Latex Conjugation Kit - 400nm Blue (ab269892) as part of examining human malaria species with multiplex lateral flow immunoassay. They used the kit to conjugate Latex to anti-(pan)pLDH antibodies for use in lateral flow assay.
Representative test strips of the two-colour LFA. Distinct colours were observed at the test lines, corresponding a type of antigens, such as a blue test lines for pLDH detection only, b mixture colour of test lines for simultaneous detection, and c red test lines for PfHRP2 detection only. The mixture colour at control lines indicated the red and blue latex particles migrated along the length of the strip

Image from Kim et al., Malar J., 18(1):313; doi: 10.1186/s12936-019-2957-x. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

• LFA

Unknown

Lateral flow assay - Latex Conjugation Kit - 400nm Blue (AB269892)

Multiplexed Latex.

The three colours of latex each conjugated to a different antibody or protein, forming a line either by a direct binding event (red and black latex) or a sandwich assay binding event (blue latex). The three colours of latex demonstrate no aggregation or background staining.

• LFA

PubMed

Lateral flow assay - Latex Conjugation Kit - 400nm Blue (AB269892)

Bond, Meaghan, et al used Latex Conjugation Kit - 400nm Blue (ab269892) as part of examining rapid tests to identify patients with sickle cell disease. They used the kit to conjugate Latex to anti-HbS and anti-HbA antibodies for use in lateral flow assay.
(a) Representative strips run with varying amounts of normal blood, including a negative control with no blood. Top image shows full-color scan; bottom image shows red channel of the same image. (b) Signal-to-background ratio of strips in part a (normal blood) and the A threshold set by previous experiment. Error bars represent ±1 SD of three strips. *10 μL represents the average of only 2 strips. (c) Representative strips run with varying amounts of sickle cell anemia (SCA) blood, including a negative control with no blood. (d) Signal-to-background ratio of strips in part c (SCA blood) and the S threshold set by previous experiment. A line present at the "A" line indicates normal, at the "S" line indicates SCA, and at neither indicates sickle cell trait (SCT). The positive control line must be present for the test to be valid.

Image from Bond et al., PLoS One, 12(5):e0177732; doi: 10.1371/journal.pone.0177732. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/