Magnetic Conjugation Kit

• Mass Spec

PubMed

Mass Spectrometry - Magnetic Conjugation Kit (AB269890)

Li, Ming Yuan, et al used Magnetic Conjugation Kit (ab269890) as part of screening for SFKs activated upon Dengue infection. They used the kit to conjugate Magnetic beads to anti-phospho-SFK antibodies for use in mass spectrometry.
(a) Large-scale immunoprecipitation of activated SFKs was performed on anti-phospho-SFK antibodies conjugated to the magnetic beads from mock- and Dengue-infected BHK21 and Vero E6 cells (MOI 2, 24 h). Isolated proteins were resolved by SDS-PAGE and detected by silver staining. (b) Entire lanes on gels were sliced into 2-mm sections and subjected to trypsin digest. The peptide mix was processed and analysed by an LTQ Orbitrap mass spectrometer. This allowed the identification of three members Src, Fyn and Lyn, and several SFK-regulated substrates in Dengue-infected samples.

Image from Li et al., Nat Commun., 11(1):5189; doi: 10.1038/s41467-020-19028-w. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

• IP

Supplier Data

Immunoprecipitation - Magnetic Conjugation Kit (AB269890)

Goat anti-rabbit was labeled with four different batches of magnetic beads. The conjugates were tested in parallel on a Rabbit IgG immunoprecipitation. The bound Rabbit IgG was eluted and quantified by 280nm absorbance.

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• WB

Supplier Data

Western blot - Magnetic Conjugation Kit (AB269890)

Rabbit IgG immunoprecipitation and detection by Western blot.

25 μg and 5 μg Goat anti-rabbit antibody were added to each of two Mini vials of magnetic particles, and to 25 μL of 1% magnetic beads from a leading competitor, and processed as per the respective protocols. The conjugates were used to immunoprecipitate rabbit IgG from rabbit serum. The eluted samples were run by SDS-PAGE and analysed by Western blot against rabbit IgG. Abcam's Magnetic Conjugation Kit has a greater binding capacity and coupling efficiency than the competitor products, and allows you to detect the antigen of interest with fewer reagents. Lane 1 : Rabbit IgG control; Lanes 2 and 4 : Competitor; Lanes 3 and 5 : Magnetic Conjugation Kit (ab269890).

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• Conjugation

Unknown

Conjugation - Magnetic Conjugation Kit (AB269890)

How The Kit Works

• Other

Supplier Data

Other - Magnetic Conjugation Kit (AB269890)

Magnetic Conjugation Kit binding capacity.

Goat anti-rabbit antibody was conjugated to 25 μL of 1% magnetic particles from Magnetic Conjugation Kit (ab269890) (1h procedure), and to magnetic particles from a leading competitor (4h procedure) as per the respective protocols. The quantity of IgG added to the magnetic particle Abcam's Magnetic Conjugation Kit demonstrates a considerably greater binding capacity.