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AB312808

mFluor™ Red 780 Conjugation Kit

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mFluor™ Red 780 Conjugation Kit (ab312808) offers several standout features:

- Rapid Conjugation: achieve mFluor™ Red 780 labeling in under 2 hours with minimal hands-on time.
- Labels up to 100 µg antibody.
- Versatility: suitable for conjugating antibodies and proteins. mFluor™ Red 780-labeled antibodies can be used immediately in Flow cytometry assays.
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Flow Cytometry - mFluor™ Red 780 Conjugation Kit (AB312808)
  • Flow Cyt

Supplier Data

Flow Cytometry - mFluor™ Red 780 Conjugation Kit (AB312808)

Flow cytometric analysis of differentiated HL-60 cells labelling CD45 using an anti-CD45 antibody labeled using the mFluor™ Red 780 Antibody Labeling Kit (ab312808). HL-60 cells were treated with 1.25% DMSO for 4 days to differentiate. Live cells were then incubated with 1 µg/mL anti-CD45-mFluor™ 780 or IgG-mFluor™ 780 control and analyzed by NovoCyte.

Key facts

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Product details

mFluor™ Red 780 Conjugation Kit provides a convenient method for labelling small amounts of monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the Red Laser-excitable mFluor™ Red 780. This dye has a fluorescence maxima (Ex/Em) of 629/780 nm respectively. These characteristics make it an excellent replacement for Alexa Fluor 700-APC tandem. In addition, mFluor™ Red 780 is a small organic molecule that is much easier to use than the Alexa Fluor 750-APC tandem. mFluor™ Red 780 is a stable dye and shows good reactivity and selectivity with protein amino groups.

This conjugation kit has all the essential components to perform two separate labelling reactions with no column purification needed. Each of the two vials of mFluor™ Red 700 provided in the kit is optimized for labelling ~50 μg antibody

Impure antibodies or antibodies stabilized with bovine serum albumin (BSA) or gelatin will not be labelled well, Abcam offers a range of purification kits

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What's included?

{ "values": { "100g": { "sellingSize": "100 µg", "publicAssetCode":"ab312808-100ug", "assetComponentDetails": [ { "size":"1 x 2 Vial", "name":"mFluor™ Red 780", "number":"AB312808-CMP01", "productcode":"" }, { "size":"1 x 20 µL", "name":"Reaction Buffer", "number":"AB312808-CMP02", "productcode":"" }, { "size":"1 x 20 µL", "name":"TQ™-Dyed Quench Buffer", "number":"AB312808-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

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