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mFluor™ Red 780 conjugation / labeling in < 4 hrs with 30 secs hands-on time using mFluor™ Red 780 Conjugation Kit ab312808. 100% antibody recovery.

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Images

Flow Cytometry - mFluor™ Red 780 Conjugation Kit (AB312808), expandable thumbnail

What's included?

100 µg
Components
Reaction Buffer
1 x 20 µL
TQ™-Dyed Quench Buffer
1 x 20 µL
mFluor™ Red 780
1 x 2 Vial

mFluor™ Red 780 conjugation / labeling in < 4 hrs with 30 secs hands-on time using mFluor™ Red 780 Conjugation Kit ab312808. 100% antibody recovery.

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

mFluor™ Red 780 Conjugation Kit provides a convenient method for labelling small amounts of monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the Red Laser-excitable mFluor™ Red 780. This dye has a fluorescence maxima (Ex/Em) of 629/780 nm respectively. These characteristics make it an excellent replacement for Alexa Fluor 700-APC tandem. In addition, mFluor™ Red 780 is a small organic molecule that is much easier to use than the Alexa Fluor 750-APC tandem. mFluor™ Red 780 is a stable dye and shows good reactivity and selectivity with protein amino groups.

This conjugation kit has all the essential components to perform two separate labelling reactions with no column purification needed. Each of the two vials of mFluor™ Red 700 provided in the kit is optimized for labelling ~50 μg antibody

Impure antibodies or antibodies stabilized with bovine serum albumin (BSA) or gelatin will not be labelled well, Abcam offers a range of purification kits

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Flow Cytometry - mFluor™ Red 780 Conjugation Kit (ab312808), expandable thumbnail

    Flow Cytometry - mFluor™ Red 780 Conjugation Kit (ab312808)

    Flow cytometric analysis of differentiated HL-60 cells labelling CD45 using an anti-CD45 antibody labeled using the mFluor™ Red 780 Antibody Labeling Kit (ab312808). HL-60 cells were treated with 1.25% DMSO for 4 days to differentiate. Live cells were then incubated with 1 µg/mL anti-CD45-mFluor™ 780 or IgG-mFluor™ 780 control and analyzed by NovoCyte.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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