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mFluor™ Violet 420 conjugation / labeling in < 4 hrs with 30 secs hands-on time using mFluor™ Violet 420 Conjugation Kit ab312805. 100% antibody recovery.

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Images

Flow Cytometry - mFluor™ Violet 420 Conjugation Kit (AB312805), expandable thumbnail

What's included?

100 µg
Components
Reaction Buffer
1 x 20 µL
TQ™-Dyed Quench Buffer
1 x 20 µL
mFluor™ Violet 420
1 x 2 Vial

mFluor™ Violet 420 conjugation / labeling in < 4 hrs with 30 secs hands-on time using mFluor™ Violet 420 Conjugation Kit ab312805. 100% antibody recovery.

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

mFluor™ Violet 420 Conjugation Kit provides a convenient method for labelling small amounts of monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the Violet Laser-excitable mFluor™ Violet 420. This dye has a fluorescence maxima (Ex/Em) of 398/411 nm respectively. These characteristics make it an excellent replacement for Cascade Blue labelling dye. mFluor™ Violet 420 is a stable dye that shows good reactivity and selectivity with protein amino groups.

This conjugation kit has all the essential components to perform two separate labelling reactions with no column purification needed. Each of the two vials of mFluor™ Violet 420 provided in the kit is optimized for labelling ~50 μg antibody

Impure antibodies or antibodies stabilized with bovine serum albumin (BSA) or gelatin will not be labelled well, Abcam offers a range of purification kits

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Flow Cytometry - mFluor™ Violet 420 Conjugation Kit (ab312805), expandable thumbnail

    Flow Cytometry - mFluor™ Violet 420 Conjugation Kit (ab312805)

    Flow cytometric analysis of human peripheral blood lymphocytes labelling CD4. Human PBMCs were stained with anti-human CD4 monoclonal antibody labelled using mFluor™ Violet 420 (ab312805). The fluorescence signal was monitored using an ACEA NovoCyte flow cytometer in the Pacific Blue channel.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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