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AB312804

mFluor™ Violet 450 Conjugation Kit

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mFluor™ Violet 450 Conjugation Kit (ab312804) offers several standout features:

- Rapid Conjugation: achieve mFluor™ Violet 450 labeling in under 2 hours with minimal hands-on time.
- Labels up to 100 µg antibody.
- Versatility: suitable for conjugating antibodies and proteins. mFluor™ Violet 450-labeled antibodies can be used immediately in Flow cytometry assays.
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Flow Cytometry - mFluor™ Violet 450 Conjugation Kit (AB312804)
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Supplier Data

Flow Cytometry - mFluor™ Violet 450 Conjugation Kit (AB312804)

Flow cytometry analysis of HL-60 cells stained with Mouse IgG control at 1 µg/mL dilution (Green) or mouse Anti-Human HLA-ABC (W6/32 mAb) at 1 µg/mL dilution (Red) followed by goat anti-mouse IgG directly conjugated with mFluor™ Violet 450 using the ReadiLink™ Rapid mFluor™ Violet 450 Antibody Labeling Kit (ab312804). The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in the Pacific Blue channel.

Key facts

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Product details

mFluor™ Violet 450 Conjugation Kit provides a convenient method for labelling small amounts of monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the Violet Laser-excitable mFluor™ Violet 450. This dye has a fluorescence maxima (Ex/Em) of 403/454 nm respectively. These characteristics make it an excellent replacement for Pacific Blue. mFluor™ Violet 450 is a stable dye that shows good reactivity and selectivity with protein amino groups.

This conjugation kit has all the essential components to perform two separate labelling reactions with no column purification needed. Each of the two vials of mFluor™ Violet 450 provided in the kit is optimized for labelling ~50 μg antibody

Impure antibodies or antibodies stabilized with bovine serum albumin (BSA) or gelatin will not be labelled well, Abcam offers a range of purification kits

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What's included?

{ "values": { "100g": { "sellingSize": "100 µg", "publicAssetCode":"ab312804-100ug", "assetComponentDetails": [ { "size":"1 x 2 Vial", "name":"mFluor™ Violet 450", "number":"AB312804-CMP01", "productcode":"" }, { "size":"1 x 20 µL", "name":"Reaction Buffer", "number":"AB312804-CMP02", "productcode":"" }, { "size":"1 x 20 µL", "name":"TQ™-Dyed Quench Buffer", "number":"AB312804-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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