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mFluor™ Violet 450 conjugation / labeling in < 4 hrs with 30 secs hands-on time using mFluor™ Violet 450 Conjugation Kit ab312804. 100% antibody recovery.

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Images

Flow Cytometry - mFluor™ Violet 450 Conjugation Kit (AB312804), expandable thumbnail

What's included?

100 µg
Components
Reaction Buffer
1 x 20 µL
TQ™-Dyed Quench Buffer
1 x 20 µL
mFluor™ Violet 450
1 x 2 Vial

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mFluor™ Violet 450 conjugation / labeling in < 4 hrs with 30 secs hands-on time using mFluor™ Violet 450 Conjugation Kit ab312804. 100% antibody recovery.

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

mFluor™ Violet 450 Conjugation Kit provides a convenient method for labelling small amounts of monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the Violet Laser-excitable mFluor™ Violet 450. This dye has a fluorescence maxima (Ex/Em) of 403/454 nm respectively. These characteristics make it an excellent replacement for Pacific Blue. mFluor™ Violet 450 is a stable dye that shows good reactivity and selectivity with protein amino groups.

This conjugation kit has all the essential components to perform two separate labelling reactions with no column purification needed. Each of the two vials of mFluor™ Violet 450 provided in the kit is optimized for labelling ~50 μg antibody

Impure antibodies or antibodies stabilized with bovine serum albumin (BSA) or gelatin will not be labelled well, Abcam offers a range of purification kits

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Flow Cytometry - mFluor™ Violet 450 Conjugation Kit (ab312804), expandable thumbnail

    Flow Cytometry - mFluor™ Violet 450 Conjugation Kit (ab312804)

    Flow cytometry analysis of HL-60 cells stained with Mouse IgG control at 1 µg/mL dilution (Green) or mouse Anti-Human HLA-ABC (W6/32 mAb) at 1 µg/mL dilution (Red) followed by goat anti-mouse IgG directly conjugated with mFluor™ Violet 450 using the ReadiLink™ Rapid mFluor™ Violet 450 Antibody Labeling Kit (ab312804). The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in the Pacific Blue channel.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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