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mFluor™ Violet 510 conjugation / labeling in < 4 hrs with 30 secs hands-on time using mFluor™ Violet 510 Conjugation Kit ab312806. 100% antibody recovery.

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Images

Flow Cytometry - mFluor™ Violet 510 Conjugation Kit (AB312806), expandable thumbnail

What's included?

100 µg
Components
Reaction Buffer
1 x 20 µL
TQ™-Dyed Quench Buffer
1 x 20 µL
mFluor™ Violet 510
1 x 2 Vial

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mFluor™ Violet 510 conjugation / labeling in < 4 hrs with 30 secs hands-on time using mFluor™ Violet 510 Conjugation Kit ab312806. 100% antibody recovery.

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

mFluor™ Violet 510 Conjugation Kit provides a convenient method for labelling small amounts of monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the Violet Laser-excitable mFluor™ Violet 510. This dye has a fluorescence maxima (Ex/Em) of 414/508 nm respectively. mFluor™ Violet 510 is a stable dye that shows good reactivity and selectivity with protein amino groups.

This conjugation kit has all the essential components to perform two separate labelling reactions with no column purification needed. Each of the two vials of mFluor™ Violet 510 provided in the kit is optimized for labelling ~50 μg antibody

Impure antibodies or antibodies stabilized with bovine serum albumin (BSA) or gelatin will not be labelled well, Abcam offers a range of purification kits

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Flow Cytometry - mFluor™ Violet 510 Conjugation Kit (ab312806), expandable thumbnail

    Flow Cytometry - mFluor™ Violet 510 Conjugation Kit (ab312806)

    Flow cytometric analysis of Jurkat cells undergoing apoptosis. Jurkat cells were treated with (red) or without 1 µM staurosporine (green) for 4 hours at 37 C. Cells were then incubated with Annexin V labeled using the mFluor™ Violet 510 Antibody Labeling Kit (ab312806) for 30 minutes to identify apoptotic cells. Fluorescence intensity was measured using an ACEA NovoCyte flow cytometer.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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