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AB312806

mFluor™ Violet 510 Conjugation Kit

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mFluor™ Violet 510 Conjugation Kit (ab312806) offers several standout features:

- Rapid Conjugation: achieve mFluor™ Violet 510 labeling in under 2 hours with minimal hands-on time.
- Labels up to 100 µg antibody.
- Versatility: suitable for conjugating antibodies and proteins. mFluor™ Violet 510-labeled antibodies can be used immediately in Flow cytometry assays.
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Flow Cytometry - mFluor™ Violet 510 Conjugation Kit (AB312806)
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Supplier Data

Flow Cytometry - mFluor™ Violet 510 Conjugation Kit (AB312806)

Flow cytometric analysis of Jurkat cells undergoing apoptosis. Jurkat cells were treated with (red) or without 1 µM staurosporine (green) for 4 hours at 37°C. Cells were then incubated with Annexin V labeled using the mFluor™ Violet 510 Antibody Labeling Kit (ab312806) for 30 minutes to identify apoptotic cells. Fluorescence intensity was measured using an ACEA NovoCyte flow cytometer.

Key facts

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Product details

mFluor™ Violet 510 Conjugation Kit provides a convenient method for labelling small amounts of monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the Violet Laser-excitable mFluor™ Violet 510. This dye has a fluorescence maxima (Ex/Em) of 414/508 nm respectively. mFluor™ Violet 510 is a stable dye that shows good reactivity and selectivity with protein amino groups.

This conjugation kit has all the essential components to perform two separate labelling reactions with no column purification needed. Each of the two vials of mFluor™ Violet 510 provided in the kit is optimized for labelling ~50 μg antibody

Impure antibodies or antibodies stabilized with bovine serum albumin (BSA) or gelatin will not be labelled well, Abcam offers a range of purification kits

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What's included?

{ "values": { "100g": { "sellingSize": "100 µg", "publicAssetCode":"ab312806-100ug", "assetComponentDetails": [ { "size":"1 x 2 Vial", "name":"mFluor™ Violet 510", "number":"AB312806-CMP01", "productcode":"" }, { "size":"1 x 20 µL", "name":"Reaction Buffer", "number":"AB312806-CMP02", "productcode":"" }, { "size":"1 x 20 µL", "name":"TQ™-Dyed Quench Buffer", "number":"AB312806-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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