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The Oligonucleotide Conjugation Kit (ab218260) enables the easy and efficient generation of antibody-oligonucleotide conjugates in less than 2 hours.
2h
The Oligonucleotide Conjugation Kit (ab218260) enables the easy and efficient generation of antibody-oligonucleotide conjugates in less than 2 hours.
2h
Ambient - Cannot Ship with Ice
Multi
Multi
Please refer to protocols
The Oligonucleotide Conjugation Kit (ab218260) enables the easy and efficient generation of antibody-oligonucleotide conjugates in less than 2 hours. The kit can be used to generate conjugates of different ratios of antibody: oligonucleotide. It also includes positive controls that allow the user to confirm that the conjugation chemistry has worked correctly.
This kit can be used to conjugate antibodies to single-stranded oligonucleotides that are 10-120 bases long or to double-stranded oligonucleotides that are up to 80 bases long. The oligonucleotide should be synthesized to include a terminal amine group.
The 1 x 100 μg kit includes reagents for 1 conjugation of 100 μg of antibody and 1 control.
The 3 x 100 μg kit includes reagents for 3 conjugations of 100 μg of antibody and 1 control.
As expected for any chemical conjugation, the concentration and buffer formulation of the oligonucleotide and the antibody need to fall within certain parameters, as detailed in the protocol booklet.
Benefits
Quick and easy to use – Save time, no specialist knowledge required
High levels of antibody and oligo recovery – Conjugation efficiency is about 95-100%.
Use your own oligo and antibody, at your desired ratio – Flexible
Freeze dried – Ships at ambient temperature, long shelf life
Stringently QC tested – Consistent high quality, excellent batch to batch reproducibility
Unidirectional chemistry – No risk of crosslinking
Covalent bond – Highly stable conjugates
Suitable for single stranded oligos of 10–120 bases, and double stranded oligos up to 80 base pairs – Covers the majority of sequences
Linking chemistry works at both 5' and 3' end – Provides ability to combine with other modifications
Post-conjugation clean-up step – No interference from unbound oligo's
Positive control antibody and oligo included – Enables confirmation of protocol success
A wide range of target proteins – Also applicable to antibody fragments and small proteins.
This product is manufactured by Expedeon, an Abcam company, and was previously called Thunder-Link® Plus Oligo Conjugation Kit. 425-0300 is the same as the 3 x 100 μg size. 425-0000 is the same as the 1 x 100 μg size.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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