Oligonucleotide Conjugation Kit

The Oligonucleotide Conjugation Kit (ab218260) enables the easy and efficient generation of antibody-oligonucleotide conjugates in less than 2 hours. The kit can be used to generate conjugates of different ratios of antibody: oligonucleotide. It also includes positive controls that allow the user to confirm that the conjugation chemistry has worked correctly.

This kit can be used to conjugate antibodies to single-stranded oligonucleotides that are 10-120 bases long or to double-stranded oligonucleotides that are up to 80 bases long. The oligonucleotide should be synthesized to include a terminal amine group.

The 1 x 100 μg kit includes reagents for 1 conjugation of 100 μg of antibody and 1 control.

The 3 x 100 μg kit includes reagents for 3 conjugations of 100 μg of antibody and 1 control.

As expected for any chemical conjugation, the concentration and buffer formulation of the oligonucleotide and the antibody need to fall within certain parameters, as detailed in the protocol booklet.

Benefits

Quick and easy to use – Save time, no specialist knowledge required

High levels of antibody and oligo recovery – Conjugation efficiency is about 95-100%.

Use your own oligo and antibody, at your desired ratio – Flexible

Freeze dried – Ships at ambient temperature, long shelf life

Stringently QC tested – Consistent high quality, excellent batch to batch reproducibility

Unidirectional chemistry – No risk of crosslinking

Covalent bond – Highly stable conjugates

Suitable for single stranded oligos of 10–120 bases, and double stranded oligos up to 80 base pairs – Covers the majority of sequences

Linking chemistry works at both 5' and 3' end – Provides ability to combine with other modifications

Post-conjugation clean-up step – No interference from unbound oligo's

Positive control antibody and oligo included – Enables confirmation of protocol success

A wide range of target proteins – Also applicable to antibody fragments and small proteins.

This product is manufactured by Expedeon, an Abcam company, and was previously called Thunder-Link^® Plus Oligo Conjugation Kit. 425-0300 is the same as the 3 x 100 μg size. 425-0000 is the same as the 1 x 100 μg size.