Rhodamine conjugation / labeling in < 20 mins with 30 secs hands-on time using Rhodamine Conjugation Kit (Fast) - Lightning-Link® ab188286. 100% antibody recovery.
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- Materials (Basel, Switzerland) 13:2020Tubular Dentin Regeneration Using a CPNE7-Derived Functional Peptide.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoon Seon Lee,Yeoung-Hyun Park,Dong-Seol Lee,You-Mi Seo,Ji-Hyun Lee,Joo-Hwang Park,Han-Wool Choung,So-Hyun Park,Won Jun Shon,Joo-Cheol ParkPubMed 33081300
- Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc 32:887-8912020A fluorescence polarization immunoassay for the rapid detection of antibody against influenza A virus in chicken and goat sera.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYohei Takeda,Yutaka Yonezawa,Satoshi Asake,Haruko Ogawa,Kunitoshi ImaiPubMed 33025860
- Cancer immunology research 8:1354-13642020A DNA-Launched Nanoparticle Vaccine Elicits CD8 T-cell Immunity to Promote Tumor Control.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZiyang Xu et. al.PubMed 32913042
- Frontiers in immunology 11:15392020Study of B Cell Repertoire in Patients With Anti-N-Methyl-D-Aspartate Receptor Encephalitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingjing Feng,Siyuan Fan,Yinwei Sun,Zhidong Zhang,Haitao Ren,Wenhan Li,Liying Cui,Bin Peng,Xiaotun Ren,Weihua Zhang,Hongzhi Guan,Jing WangPubMed 32849520
- Frontiers in immunology 11:14942020Type I Interferon Regulates the Survival and Functionality of B Cells in Rainbow Trout.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOttavia Benedicenti et. al.PubMed 32733485
- Cells 9:2020Nrp1 is Activated by Konjac Ceramide Binding-Induced Structural Rigidification of the a1a2 Domain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeigo Usuki et. al.PubMed 32102436
- mBio 10:2019A Porcine Lung Perfusion Model To Investigate Bacterial Pathogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmy Dumigan et. al.PubMed 31796543
- Journal of dental research 98:1239-12442019CPNE7 Induces Biological Dentin Sealing in a Dentin Hypersensitivity Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS H Park,Y S Lee,D S Lee,J C Park,R Kim,W J ShonPubMed 31425664
- Nature microbiology 4:459-4692019TesG is a type I secretion effector of Pseudomonas aeruginosa that suppresses the host immune response during chronic infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKelei Zhao et. al.PubMed 30617346
- Biosensors & bioelectronics 130:330-3372018Development and application of a novel electrochemical immunosensor for tetracycline screening in honey using a fully integrated electrochemical Bio-MEMS.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNadia El Alami El Hassani,Abdoullatif Baraket,Selim Boudjaoui,Ernandes Taveira Tenório Neto,Joan Bausells,Nezha El Bari,Benachir Bouchikhi,Abdelhamid Elaissari,Abdelhamid Errachid,Nadia ZinePubMed 30287175
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Rhodamine conjugation / labeling in < 20 mins with 30 secs hands-on time using Rhodamine Conjugation Kit (Fast) - Lightning-Link® ab188286. 100% antibody recovery.
Storage
- Shipped at conditions
- Ambient - Cannot Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Rhodamine Conjugation Kit / Rhodamine Labeling Kit ab188286 uses a simple and quick process for rhodamine labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides. Learn about our . To conjugate an antibody to Rhodamine using this kit: - add modifier to antibody and incubate for 15 mins - add quencher and incubate for 5 mins The rhodamine conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use. The excitation and emmision wavelengths for Rhodamine are Ex: 550nm, Em: 570nm. Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid . Use the to learn more about the technology, or about conjugating other proteins and peptides to Rhodamine. Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.
This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link® Rapid Rhodamine Labeling Kit. 311-0005 is the same as the 100 ug size. 311-0010 is the same as the 3 x 100 ug size. 311-0030 is the same as the 3 x 10 ug size. 311-0015 is the same as the 1 mg size.
Amount and volume of antibody for conjugation to Rhodamine
| Kit size | Recommended amount of antibody1 | Maximum amount of antibody | Maximum antibody volume2 |
| 3 x 10 μg | 3 x 10 μg | 3 x 20 μg | 3 x 10 μL |
| 100 μg | 1 x 100 μg | 1 x 200 μg | 1 x 100 μL |
| 3 x 100 μg | 3 x 100 μg | 3 x 200 μg | 3 x 100 μL |
| 1 mg | 1 x 1 mg | 1 x 2 mg | 1 x 1 mL |
1 Using the maximum amount of antibody may result in less labelling per antibody.
2 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 2 mg/ml or < 0.5 mg/ml should be diluted /concentrated.
Buffer Requirements for Conjugation
Buffer should be pH 6.5-8.5.
Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.
| 50mM / 0.6% Tris1 | 0.1% BSA2 | 50% glycerol |
| 0.1% sodium azide | PBS | Potassium phosphate |
| Sodium chloride | HEPES | Sucrose |
| Sodium citrate | EDTA | Trehalose |
1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
2 BSA can also interfere with the use of the conjugated antibody in tissue staining.
Incompatible buffer constituents
| Thiomerosal | Proclin | Glycine |
| Arginine | Glutathione | DTT |
If a constituent of the buffer containing your antibody or protein is not listed above, please contact us.
Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.
Storing and handling conjugation kits
Lyophilized Lightning-Link® components are hygroscopic.
Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.
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4 product images
Image from Dumigan, Amy, et al., MBio., 10(6): e02802-19; doi: 10.1128/mBio.02802-19. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/Flow Cytometry - Rhodamine Conjugation Kit (Fast) - Lightning-Link® (ab188286)
Rhodamine Conjugation Kit (Fast) - Lightning-Link®.
Dumigan, Amy, et al used Rhodamine Conjugation Kit (Fast) - Lightning-Link® (ab188286) as part of examining innate cell recruitment in K. pneumoniae-infected porcine EVLP model. They used the kit to conjugate Rhodamine to anti-pig granulocyte marker antibody, clone 6D10, for use in flow cytometry.
Gating strategy and representative dot plots for flow cytometric analysis of neutrophil staining using anti-pig granulocyte marker clone 6D10 (B). Dot plots represent 0-h (baseline) and 4-h-postinfection or mock infection BAL samples and 4-h tissue samples.
Image from Terrell-Hall et al., Oncotarget., 8(48):83734-83744; doi: 10.18632/oncotarget.19634. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/3.0/Conjugation - Rhodamine Conjugation Kit (Fast) - Lightning-Link® (ab188286)
Terrell-Hall, Tori B., et al used Rhodamine Conjugation Kit (Fast) - Lightning-Link� (ab188286) as part of examining mechanism of trastuzumab movement. They used the kit to conjugate Rhodamine to trastuzumab for characterization in a novel microfluidic in-vitro.
Linear central compartment accumulation of trastuzumab-Rhodamine123 (t-Rho123) in in-vitro blood-brain barrier (BBB) and blood-tumor barrier (BTB) microfluidic chip models. Representative image of model with TRITC labeled t-Rho123 flowing over HUVEC cells in the outer compartment and either astrocytes or JIMT-1 cancer cells in the central compartment (A). Rate of t-Rho123 movement in each model plotted against the unrestricted diffusion kin; ** p<0.0033 significance between BBB model and unrestricted diffusion kin, n=3; *** p<0.0005 significance between BTB model and unrestricted diffusion kin, n=3. All data represent mean � S.E.M. Each model is significantly different than 0 (p < 0.05) (B). Representative graphs of the rate of accumulation of t-Rho123 in the BBB (C) and BTB (D) microfluidic devices (n?3).
Image from Usuki, Seigo, et al., Cells, 9(2):517; doi: 10.3390/cells9020517. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/Fluorescence Microscopy - Rhodamine Conjugation Kit (Fast) - Lightning-Link® (ab188286)
Usuki, Seigo, et al used Rhodamine Conjugation Kit (Fast) - Lightning-Link® (ab188286) as part of examining the time course of NBD-ceramides (NBD-Cer) and Rhodamine-bovine serum albumin (Rhod-BSA) bound to PC12 cells. . They used the kit to conjugate Rhodamine to bovine serum albumin (BSA) for use in Dissociation Time Course for Cell Surface Binding.
(A) Dissociation time course analysis of FI based on binding of 100 nM d NBD-Cer (FI = 5000) and 100 nM Rhod-BSA (FI = 5510), examined using Plexin A1 gene-silencing PC12 cells. (B) Images showing (1 to 4) changes in d4t,8t-NBD-Cer and (5 to 8) changes in Rhod-BSA at the indicated timepoints. The left graph shows a time course plot of FI (%) relative to 0 min. Data are presented as mean ± SD (n = 3). Scale bar = 100 μm. (C) Images showing (1 to 4) changes in d4t,8c-NBD-Cer and (5 to 8) changes in Rhod-BSA at the indicated timepoints. The left graph is a time course plot of FI (%) relative to 0 min. Data are presented as mean ± SD (n = 3). Scale bar = 100 μm.
Image from Wills, Jonathan, et al., PLoS One, 6(3): e17953; doi: 10.1371/journal.pone.0017953. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/Immunohistochemistry (PFA perfusion fixed frozen sections) - Rhodamine Conjugation Kit (Fast) - Lightning-Link® (ab188286)
Wills, Jonathan, et al used Rhodamine Conjugation Kit (Fast) - Lightning-Link® (ab188286) as part of examining MC1 immunostaining in the striatum of A53T α-Syn mutant mice (A53T Tg) and wild-type mice (WT). They used the kit to conjugate Rhodamine to anti-MC1 antibody for use in immunohistochemistry (PFA perfusion fixed frozen sections).
Sections of striatum of A53T Tg and age-matched WT mice were stained with anti-MC1 antibody to detect p-Tau conformation (red). Nuclei were stained with DAPI (blue). Slides are shown at lowest magnification (upper panels) to highest magnification (lower panels). Asterisks indicate highlighted individual cells shown at higher magnification in lower panels. Scale Bar: 10 μm.
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