Rhodamine Conjugation Kit (Fast) - Lightning-Link®

• Schematic Diagram

Supplier Data

Schematic Diagram - Rhodamine Conjugation Kit (Fast) - Lightning-Link® (AB188286)

This illustration demonstrates a general procedure of how the Lightning-Link®(Fast) labeling technology enables the direct labeling of antibodies or proteins.

Simply pipette your antibody or biomolecule of choice into the vial of a lyophilized mixture containing the label of interest and incubate for just 15 minutes. Please see the ab188286 protocol booklet for more details.

Learn more about our Lightning-Link® conjugation kits here

• IHC-FoFr

PubMed

Immunohistochemistry (PFA perfusion fixed frozen sections) - Rhodamine Conjugation Kit (Fast) - Lightning-Link® (AB188286)

Wills, Jonathan, et al used Rhodamine Conjugation Kit (Fast) - Lightning-Link® (ab188286) as part of examining MC1 immunostaining in the striatum of A53T α-Syn mutant mice (A53T Tg) and wild-type mice (WT). They used the kit to conjugate Rhodamine to anti-MC1 antibody for use in immunohistochemistry (PFA perfusion fixed frozen sections).
Sections of striatum of A53T Tg and age-matched WT mice were stained with anti-MC1 antibody to detect p-Tau conformation (red). Nuclei were stained with DAPI (blue). Slides are shown at lowest magnification (upper panels) to highest magnification (lower panels). Asterisks indicate highlighted individual cells shown at higher magnification in lower panels. Scale Bar : 10 μm.

Image from Wills, Jonathan, et al., PLoS One, 6(3): e17953; doi: 10.1371/journal.pone.0017953. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

• Fluorescence Microscopy

PubMed

Fluorescence Microscopy - Rhodamine Conjugation Kit (Fast) - Lightning-Link® (AB188286)

Usuki, Seigo, et al used Rhodamine Conjugation Kit (Fast) - Lightning-Link^® (ab188286) as part of examining the time course of NBD-ceramides (NBD-Cer) and Rhodamine-bovine serum albumin (Rhod-BSA) bound to PC12 cells. . They used the kit to conjugate Rhodamine to bovine serum albumin (BSA) for use in Dissociation Time Course for Cell Surface Binding.
(A) Dissociation time course analysis of FI based on binding of 100 nM d NBD-Cer (FI = 5000) and 100 nM Rhod-BSA (FI = 5510), examined using Plexin A1 gene-silencing PC12 cells. (B) Images showing (1 to 4) changes in d4t,8t-NBD-Cer and (5 to 8) changes in Rhod-BSA at the indicated timepoints. The left graph shows a time course plot of FI (%) relative to 0 min. Data are presented as mean ± SD (n = 3). Scale bar = 100 μm. (C) Images showing (1 to 4) changes in d4t,8c-NBD-Cer and (5 to 8) changes in Rhod-BSA at the indicated timepoints. The left graph is a time course plot of FI (%) relative to 0 min. Data are presented as mean ± SD (n = 3). Scale bar = 100 μm.

Image from Usuki, Seigo, et al., Cells, 9(2):517; doi: 10.3390/cells9020517. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

• Flow Cyt

PubMed

Flow Cytometry - Rhodamine Conjugation Kit (Fast) - Lightning-Link® (AB188286)

Rhodamine Conjugation Kit (Fast) - Lightning-Link®.

Dumigan, Amy, et al used Rhodamine Conjugation Kit (Fast) - Lightning-Link® (ab188286) as part of examining innate cell recruitment in K. pneumoniae-infected porcine EVLP model. They used the kit to conjugate Rhodamine to anti-pig granulocyte marker antibody, clone 6D10, for use in flow cytometry.
Gating strategy and representative dot plots for flow cytometric analysis of neutrophil staining using anti-pig granulocyte marker clone 6D10 (B). Dot plots represent 0-h (baseline) and 4-h-postinfection or mock infection BAL samples and 4-h tissue samples.

Image from Dumigan, Amy, et al., MBio., 10(6): e02802-19; doi: 10.1128/mBio.02802-19. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

• Conjugation

PubMed

Conjugation - Rhodamine Conjugation Kit (Fast) - Lightning-Link® (AB188286)

Terrell-Hall, Tori B., et al used Rhodamine Conjugation Kit (Fast) - Lightning-Link® (ab188286) as part of examining mechanism of trastuzumab movement. They used the kit to conjugate Rhodamine to trastuzumab for characterization in a novel microfluidic in-vitro.

Linear central compartment accumulation of trastuzumab-Rhodamine123 (t-Rho123) in in-vitro blood-brain barrier (BBB) and blood-tumor barrier (BTB) microfluidic chip models. Representative image of model with TRITC labeled t-Rho123 flowing over HUVEC cells in the outer compartment and either astrocytes or JIMT-1 cancer cells in the central compartment (A). Rate of t-Rho123 movement in each model plotted against the unrestricted diffusion kin; ** p<0.0033 significance between BBB model and unrestricted diffusion kin, n=3; *** p<0.0005 significance between BTB model and unrestricted diffusion kin, n=3. All data represent mean ± S.E.M. Each model is significantly different than 0 (p < 0.05) (B). Representative graphs of the rate of accumulation of t-Rho123 in the BBB (C) and BTB (D) microfluidic devices (n≥3).

Image from Terrell-Hall et al., Oncotarget., 8(48):83734-83744; doi: 10.18632/oncotarget.19634. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/3.0/