SimpleStep ELISA® CaptSure™ Peptide Conjugation Kit

Product details

SimpleStep ELISA^® CaptSure™ Peptide Conjugation Kit (ab270690) allows the CaptSure™ Peptide conjugation to be set up in less than 30 seconds, simply by adding a solution of the antibody to a lyophilized mixture containing a proprietary activated peptide.

By circumventing the desalting or dialysis steps that commonly interrupt traditional antibody conjugation procedures, this process can be used to label both small (e.g. 20 μg) and large quantities of primary antibodies with ease. Batch-to-batch variation upon scale-up is minimal as the process is so simple, and recoveries are always 100%.

It can also be used to label proteins, peptides and other biomolecules.

Capture antibodies tagged with CaptSure™ Peptide are paired with detection antibodies labeled with HRP to be then used within SimpleStep ELISA^® Custom ELISA Kit (ab270552). Upon addition to the Assay Plate, the non-biological CaptSure™ Peptide is bound with high affinity to the antibody, pre-coated on the plates, and the immuno-complex is thus immobilized into the well.

This technology works by targeting free amine groups. It can be used to label antibodies, peptides, proteins and other molecules with free amine groups.