Streptavidin Antibody Conjugation Check Kit

• FuncS

Supplier Data

Functional Studies - Streptavidin Antibody Conjugation Check Kit (AB270051)

Streptavidin Antibody Conjugation Check Kit.

Rabbit IgG has been conjugated with to Streptavidin and run on Protein A / G strips at different concentrations together with 0.2OD Gold conjugated Biotin. The signal intensity varies based on the antibody species.

• FuncS

Supplier Data

Functional Studies - Streptavidin Antibody Conjugation Check Kit (AB270051)

Streptavidin Antibody Conjugation Check Kit.

Signal intensity of different Streptavidin–Antibody conjugates

• Schematic Diagram

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Schematic Diagram - Streptavidin Antibody Conjugation Check Kit (AB270051)

Streptavidin Antibody Conjugation Check Kit.

How the kit works :

The nitrocellulose membrane contains a 'Test line' of immobilized Protein A and Protein G with a high affinity for the Fc region of a variety of IgG molecules. The strips also contain an absorbent pad to promote and control the flow of sample through the nitrocellulose. The streptavidin-antibody conjugate is run on the Protein A/G strip together with 40 nm gold conjugated Biotin as detection label. Biotin binds streptavidin with exceptionally high affinity and when the streptavidin-antibody conjugate flows along the nitrocellulose membrane, upon binding to the Protein A and Protein G on the T-line, a visible red line appears on the strip.