Labeling in < 4 hrs with 30 secs hands-on time using Streptavidin AZDye™ 594 Monovalent Antibody Labeling Kit ab272189. 100% antibody recovery.
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- Cardiovascular research 119:1265-12782022Endothelial PTP4A1 mitigates vascular inflammation via USF1/A20 axis-mediated NF-κB inactivation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Ji Cho,Dong Gwang Lee,Jeong Woong Lee,Byungtae Hwang,Sung-Jin Yoon,Seon-Jin Lee,Young-Jun Park,Seung-Ho Park,Hee Gu Lee,Yong-Hoon Kim,Chul-Ho Lee,Jangwook Lee,Nam-Kyung Lee,Tae-Su Han,Hyun-Soo Cho,Jeong Hee Moon,Ga Seul Lee,Kwang-Hee Bae,Geum-Sook Hwang,Sang-Hak Lee,Sang J Chung,Sungbo Shim,Jaehyung Cho,Goo Taeg Oh,Young-Guen Kwon,Jong-Gil Park,Jeong-Ki MinPubMed 36534975
- Scientific reports 12:74072022Achievement of complete in vitro spermatogenesis in testicular tissues from prepubertal mice exposed to mono- or polychemotherapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarion Delessard,Laura Stalin,Aurélie Rives-Feraille,Laura Moutard,Justine Saulnier,Ludovic Dumont,Nathalie Rives,Christine RondaninoPubMed 35523907
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Flow Cyt | Reactivity Reacts | Dilution info - | Notes - |
Application IHC | Reactivity Reacts | Dilution info - | Notes - |
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Labeling in < 4 hrs with 30 secs hands-on time using Streptavidin AZDye™ 594 Monovalent Antibody Labeling Kit ab272189. 100% antibody recovery.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Streptavidin AZDye™ 594 Monovalent Antibody Labeling Kit ab272189 is used to label biotinylated antibodies with AZDye™ 594.
The Antibody - Streptavidin AZDye™ 594 complex produced with this kit is stable and can be used in the same way as antibodies conjugated directly with fluorochromes.
Several antibodies can be labeled with this kit, and its associated kits, and used together in the same experiment.
Use this kit to:
- label biotinylated antibodies with fluorescent dyes, and use them in immunostaining just like a directly dye conjugated antibody
- label two or more biotinylated antibodies with different fluorescent dyes* and then use them together in the same experiment
*multiple kits required, see list below
- label as little as 0.1 μg of biotinylated antibody in less than 5 mins without washes
Technical notes
The 50 μg size of this kit is typically sufficient to label 50 μg of antibody, the 25 μg size is sufficient to label 25 μg of antibody.
When the antibody - streptavidin complex is produced with this kit, due to the monovalent nature of the streptavidin and the blocking solution, there is no risk of forming aggregates of multiple antibodies.
The antibody - streptavidin complex is also very stable, if 2 or more biotinylated antibodies are labeled with different dyes, and then mixed, no dye will transfer between the antibodies.
List of available monovalent streptavidin antibody labeling kits
Streptavidin Alexa Fluor® 488 Equivalent (Streptavidin AZDye™ 488 Monovalent Antibody Labeling Kit ab272187)
Streptavidin Atto 542 (Streptavidin Atto 542 Monovalent Antibody Labeling Kit ab272188)
Streptavidin AZDye™ 594 (ab272189) – this kit
Streptavidin AZDye™ 647 Equivalent (Streptavidin AZDye™ 647 Monovalent Antibody Labeling Kit ab272190)
Note: the streptavidin in this kit contains very small amounts of non-monovalent streptavidin, when used with the blocking buffer included in the kit, this does not affect the conjugated antibodies.
This product was previously called Streptavidin Alexa Fluor® 594 Antibody Labeling Kit.
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3 product images
Flow Cytometry - Streptavidin AZDye™ 594 Monovalent Antibody Labeling Kit (ab272189)
Brightness compared to directly conjugated antibodies.
Antibodies labeled with Streptavidin (Monovalent) kits are as bright as directly conjugated antibodies. Flow cytometry analysis of splenic B lymphocytes immunolabeled with antibodies against CD45R (B220, clone RA3-6B2, 7 μg/ml). Dark green = directly conjugated antibody, light green = Streptavidin (Monovalent)-labeled antibody.
Immunofluorescence - Streptavidin AZDye™ 594 Monovalent Antibody Labeling Kit (ab272189)
Multiplex 4 color staining.
Multiplex immunostaining of a frozen tissue section of mouse spleen using four biotinylated antibodies labeled using Streptavidin (Monovalent) kits. T cells (rat anti-CD3, 7 μg/ml, green), B cells (rat anti-CD45R, 7 μg/ml, red), macrophages (rat anti-CD11b, 7 μg/ml, white), and dendritic cells (hamster anti-CD11c, 7 μg/ml, blue) were detected using Streptavidin (Monovalent) kits with four different fluorochromes. Picture was taken with a standard fluorescence microscope equipped with appropriate filters for optimal multicolor separation.
Flow Cytometry - Streptavidin AZDye™ 594 Monovalent Antibody Labeling Kit (ab272189)
No spillover of signals from different labels.
No spillover of signals between Streptavidin (Monovalent)-labeled antibodies. Flow cytometry analysis of T and B cells immunolabeled (7 μg/ml) with Streptavidin Alexa Fluor® 647 (Monovalent)(Streptavidin AZDye™ 647 Monovalent Antibody Labeling Kit ab272190) and Streptavidin Alexa Fluor® 488 (Monovalent)(Streptavidin AZDye™ 488 Monovalent Antibody Labeling Kit ab272187).
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