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Streptavidin conjugation / labeling in < 4 hrs with 30 secs hands-on time using Streptavidin Conjugation Kit - Lightning-Link® ab102921. 100% antibody recovery.

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What's included?

3 x 10 µg
Components
Modifier reagent
1 x 200 µL
Quencher reagent
1 x 200 µL
Streptavidin mix
3 x 10 µg

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Streptavidin conjugation / labeling in < 4 hrs with 30 secs hands-on time using Streptavidin Conjugation Kit - Lightning-Link® ab102921. 100% antibody recovery.

Storage

Shipped at conditions

Ambient - Cannot Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Streptavidin Conjugation Kit / Streptavidin Labeling Kit ab102921 uses a simple and quick process for streptavidin labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides. Learn about our . To conjugate an antibody to Streptavidin using this kit: - add modifier to antibody and incubate for 3 hrs - add quencher and incubate for 30 mins The conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use. Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid . Use the  to learn more about the technology, or about conjugating other proteins and peptides to Streptavidin. Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.

This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link® Streptavidin Labeling Kit. 708-0005 is the same as the 100 μg size. 708-0010 is the same as the 3 x 100 ug size. 708-0030 is the same as the 3 x 10 ug size. 708-0015 is the same as the 1 mg size.

Amount and volume of antibody for conjugation to Streptavidin

Kit sizeRecommended maximum
amount of antibody
Maximum antibody
volume1
3 x 10 μg3 x 10 μg3 x 10 μL
100 μg100 μg100 μL
3 x 100 μg3 x 100 μg3 x 100 μL
1 x 1 mg1 x 1 mg1 x 1 mL

1 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 5mg/ml or < 0.5 mg/ml should be diluted /concentrated.

Buffer Requirements for Conjugation

Buffer should be pH 6.5-8.5.

Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.

50mM / 0.6% Tris10.1% BSA50% glycerol
0.1% sodium azidePBSPotassium phosphate
Sodium chlorideHEPESSucrose
Sodium citrateEDTATrehalose

1 Tris buffered saline is almost always ≤ 50 mM / 0.6%

Incompatible buffer constituents

ThiomerosalProclinGlycine
ArginineGlutathioneDTT

If a constituent of the buffer containing your antibody or protein is not listed above, please contact us.

Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture are not suitable for conjugation.

Storing and handling conjugation kits

Lyophilized Lightning-Link® components are hygroscopic.

Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Conjugation - Streptavidin Conjugation Kit - Lightning-Link® (ab102921), expandable thumbnail
    Image from Pereira et al., Traffic, 21(5):375-385; doi: 10.1111/tra.12728. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    Conjugation - Streptavidin Conjugation Kit - Lightning-Link® (ab102921)

    Pereira, Pedro M., et al used Streptavidin Conjugation Kit - Lightning-Link® (ab102921) as part of examining super-resolution fluorescent probes for Single-molecule localization microscopy. They used the kit to conjugate Streptavidin to anti-mouse secondary antibody for use in fixed-cell imaging.
    D, Pre-acquisition snapshot (WF) and SMLM reconstruction (STORM) of β-tubulin immunolabelling with B3S9L SB (B3S9L-ATTO555-BHQ2) and control probe (B3S9L-ATTO555). Scale bars are 5 μm. E, Schematic representation of the strategy used to perform β-tubulin immunolabelling (left), measurement of Full With Half Maximum (FWHM) and distances between structures on the highlighted regions in D

  • Conjugation - Streptavidin Conjugation Kit - Lightning-Link® (ab102921), expandable thumbnail
    Image from Hoffmann et al., Sci Rep., 10(1):8869; doi: 10.1038/s41598-020-65860-x.Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    Conjugation - Streptavidin Conjugation Kit - Lightning-Link® (ab102921)

    Hoffmann, Ricarda M., et al used Streptavidin Conjugation Kit - Lightning-Link® (ab102921) as part of examining development of anticancer Antibody-Drug Conjugates. They used the kit to conjugate Streptavidin to Trastuzumab and trastuzumab emtansine antibodies for use in streptavidin-biotin based assay.
    Investigation of cell viability upon treatment with the naked antibody (black), antibody-Streptavidin-Biotin-Saporin conjugate (orange) or Saporin alone (grey). The ribosome inhibitor Saporin, unable to enter the cell alone, can be used to investigate antibody internalization by measuring viability (MTS) of antibody-SB-Saporin conjugate-treated cells.

  • Conjugation - Streptavidin Conjugation Kit - Lightning-Link® (ab102921), expandable thumbnail
    Image from Wang et al., Redox Biol. 24:101153; doi: 10.1016/j.redox.2019.101153. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    Conjugation - Streptavidin Conjugation Kit - Lightning-Link® (ab102921)

    Wang, Xinzhi et al used Streptavidin Conjugation Kit - Lightning-Link® (ab102921) as part of examining. They used the kit to conjugate Streptavidin to recombinant proteins for use in bio-layer interferometry.
    Comparison of the binding activity between PepE and TrxR1 protein (red line), PepE and TrxR1 nSec498 protein (blue line), and PepA and TrxR1 protein (black line) through BLI analysis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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