Streptavidin Conjugation Kit - Lightning-Link®

• Conjugation

PubMed

Conjugation - Streptavidin Conjugation Kit - Lightning-Link® (AB102921)

Pereira, Pedro M., et al used Streptavidin Conjugation Kit - Lightning-Link^® (ab102921) as part of examining super-resolution fluorescent probes for Single-molecule localization microscopy. They used the kit to conjugate Streptavidin to anti-mouse secondary antibody for use in fixed-cell imaging.
D, Pre-acquisition snapshot (WF) and SMLM reconstruction (STORM) of β-tubulin immunolabelling with B3S9L SB (B3S9L-ATTO555-BHQ2) and control probe (B3S9L-ATTO555). Scale bars are 5 μm. E, Schematic representation of the strategy used to perform β-tubulin immunolabelling (left), measurement of Full With Half Maximum (FWHM) and distances between structures on the highlighted regions in D

Image from Pereira et al., Traffic, 21(5):375-385; doi: 10.1111/tra.12728. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

• Schematic Diagram

Supplier Data

Schematic Diagram - Streptavidin Conjugation Kit - Lightning-Link® (AB102921)

This illustration demonstrates a general procedure of how Lightning-Link® labeling technology enables the direct labeling of antibodies or proteins.

Simply pipette your antibody or biomolecule of choice into the vial of a lyophilized mixture containing the label of interest and incubate for around 3 hours. Please see the ab102921 protocol booklet for more details.

Learn more about our Lightning-Link® conjugation kits here

• Conjugation

PubMed

Conjugation - Streptavidin Conjugation Kit - Lightning-Link® (AB102921)

Wang, Xinzhi et al used Streptavidin Conjugation Kit - Lightning-Link^® (ab102921) as part of examining. They used the kit to conjugate Streptavidin to recombinant proteins for use in bio-layer interferometry.
Comparison of the binding activity between PepE and TrxR1 protein (red line), PepE and TrxR1 nSec498 protein (blue line), and PepA and TrxR1 protein (black line) through BLI analysis.

Image from Wang et al., Redox Biol. 24:101153; doi: 10.1016/j.redox.2019.101153. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

• Conjugation

PubMed

Conjugation - Streptavidin Conjugation Kit - Lightning-Link® (AB102921)

Hoffmann, Ricarda M., et al used Streptavidin Conjugation Kit - Lightning-Link^® (ab102921) as part of examining development of anticancer Antibody-Drug Conjugates. They used the kit to conjugate Streptavidin to Trastuzumab and trastuzumab emtansine antibodies for use in streptavidin-biotin based assay.
Investigation of cell viability upon treatment with the naked antibody (black), antibody-Streptavidin-Biotin-Saporin conjugate (orange) or Saporin alone (grey). The ribosome inhibitor Saporin, unable to enter the cell alone, can be used to investigate antibody internalization by measuring viability (MTS) of antibody-SB-Saporin conjugate-treated cells.

Image from Hoffmann et al., Sci Rep., 10(1):8869; doi: 10.1038/s41598-020-65860-x.Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/