Texas Red® Conjugation Kit (Fast) - Lightning-Link®
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(7 Publications)
- Rapid Conjugation: achieve Texas Red® labeling in under 20 minutes with just 30 seconds of hands-on time.
- High Efficiency: ensures 100% antibody recovery, meaning no loss of valuable antibodies.
- Versatility: suitable for conjugating antibodies, proteins, and peptides. Texas Red® -labeled antibodies can be used immediately in applications such as Flow cytometry, ELISA, and Immunohistochemistry (IHC) without further purification.
- Schematic Diagram
Supplier Data
Schematic Diagram - Texas Red® Conjugation Kit (Fast) - Lightning-Link® (AB195225)
This illustration demonstrates a general procedure of how the Lightning-Link®(Fast) labeling technology enables the direct labeling of antibodies or proteins.
Simply pipette your antibody or biomolecule of choice into the vial of a lyophilized mixture containing the label of interest and incubate for just 15 minutes. Please see the ab195225 protocol booklet for more details.
Learn more about our Lightning-Link® conjugation kits here
- IHC-FoFr
PubMed
Immunohistochemistry (PFA perfusion fixed frozen sections) - Texas Red® Conjugation Kit (Fast) - Lightning-Link® (AB195225)
Wills, Jonathan, et al used FITC Conjugation Kit - Lightning-Link® (ab102884) as part of examining the aggregation and co-localization of α-Syn with pGSK-3β and p-Tau in the striatum of A53T α-Syn mutant mice (A53T Tg) and wild-type mice (WT). They used the kit to conjugate FITC to Anti-p-GSK-3β (pY216) antibody for use in immunohistochemistry (PFA perfusion fixed frozen sections).
Right panels constitute merged image of left panels. Sections of striatum of A53T Tg and age-matched WT mice were stained with anti-α-Syn antibody conjugated to Texas Red (red) and anti-p-GSK3β conjugated to FITC (green). Nuclei were stained with DAPI (blue).
Image from Wills, Jonathan, et al., PLoS One, 6(3): e17953; doi: 10.1371/journal.pone.0017953. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
- Flow Cyt
PubMed
Flow Cytometry - Texas Red® Conjugation Kit (Fast) - Lightning-Link® (AB195225)
Guerrero-Arguero, Israel, et al used Texas Red® Conjugation Kit (Fast) - Lightning-Link® (ab195225) as part of examining the rate of productive Chikungunya virus (CHIKV) replication via flow cytometry. They used the kit to conjugate Texas Red® to anti-Chikungunya E1 glycoprotein antibody for use in flow cytometry.
PMA-differentiated U937 and RAW264.7 cells display CHIKV envelope proteins at 6 hpi. PMA = phorbol 12-mystrate 13-acetate
PMA-differentiated U937 and RAW264.7 macrophages were exposed for 2 hours to CHIKV and then fixed and assayed at 8 hpi using flow cytometry and an anti-E1 protein fluorophore-conjugated monoclonal antibody. Data show mean values of three independent experiments with a total of n = 9, MOI = 1. Statistical significance was determined using multiple t-test corrected using Holm-Sidak method. *P<0.05; NS, not significant.
Image from Guerrero-Arguero, Israel, et al., PloS one, 15(3): e0230328; doi: 10.1371/journal.pone.0230328. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
Product details
Texas Red® Conjugation Kit / Texas Red Labeling Kit ab195225 uses a simple and quick process for Texas Red® labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides. Learn about our antibody labeling kits and their advantages.
To conjugate an antibody to Texas Red® using this kit:
- add modifier to antibody and incubate for 15 mins
- add quencher and incubate for 5 mins
The Texas Red conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.
Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to Texas Red®.
Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.
This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link® Rapid Texas Red® Labeling Kit. 315-0005 is the same as the 100 ug size. 315-0010 is the same as the 3 x 100 ug size. 315-0030 is the same as the 3 x 10 ug size. 315-0015 is the same as the 1 mg size.
Amount and volume of antibody for conjugation to Texas Red®
| Kit size | Recommended amount of antibody1 |
Maximum amount of antibody |
Maximum antibody volume2 |
| 3 x 10 μg | 3 x 10 μg | 3 x 20 μg | 3 x 10 μL |
| 100 μg | 1 x 100 μg | 1 x 200 μg | 1 x 100 μL |
| 3 x 100 μg | 3 x 100 μg | 3 x 200 μg | 3 x 100 μL |
| 1 mg | 1 x 1 mg | 1 x 2 mg | 1 x 1 mL |
1 Using the maximum amount of antibody may result in less labelling per antibody.
2 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 2 mg/ml or < 0.5 mg/ml should be diluted /concentrated.
Buffer Requirements for Conjugation
Buffer should be pH 6.5-8.5.
Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.
| 50mM / 0.6% Tris1 | 0.1% BSA2 | 50% glycerol |
| 0.1% sodium azide | PBS | Potassium phosphate |
| Sodium chloride | HEPES | Sucrose |
| Sodium citrate | EDTA | Trehalose |
1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
2 BSA can also interfere with the use of the conjugated antibody in tissue staining.
Incompatible buffer constituents
| Thiomerosal | Proclin | Glycine |
| Arginine | Glutathione | DTT |
If a constituent of the buffer containing your antibody or protein is not listed above, please contact us.
Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.
Storing and handling conjugation kits
Lyophilized Lightning-Link® components are hygroscopic.
Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
- Download websiteProtocolBooklet|zh
Target data
Publications (7)
Recent publications for all applications. Explore the full list and refine your search
JCI insight 7: PubMed35862218
2022
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Nature communications 13:2540 PubMed35534484
2022
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eLife 10: PubMed33570494
2021
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PloS one 15:e0230328 PubMed32163514
2020
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Journal of proteome research 18:3404-3418 PubMed31335145
2019
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The European journal of neuroscience 33:1598-610 PubMed21453448
2011
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PloS one 6:e17953 PubMed21445308
2011
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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