Texas Red® conjugation / labeling in < 20 mins with 30 secs hands-on time using Texas Red® Conjugation Kit (Fast) - Lightning-Link® ab195225. 100% antibody recovery.
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- JCI insight 7:2022ARMC5-CUL3 E3 ligase targets full-length SREBF in adrenocortical tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYosuke Okuno,Atsunori Fukuhara,Michio Otsuki,Iichiro ShimomuraPubMed 35862218
- Nature communications 13:25402022Spatial epitranscriptomics reveals A-to-I editome specific to cancer stem cell microniches.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmos C Lee,Yongju Lee,Ahyoun Choi,Han-Byoel Lee,Kyoungseob Shin,Hyunho Lee,Ji Young Kim,Han Suk Ryu,Hoe Suk Kim,Seung Yeon Ryu,Sangeun Lee,Jong-Ho Cheun,Duck Kyun Yoo,Sumin Lee,Hansol Choi,Taehoon Ryu,Huiran Yeom,Namphil Kim,Jinsung Noh,Yonghee Lee,Inyoung Kim,Sangwook Bae,Jinhyun Kim,Wooseok Lee,Okju Kim,Yushin Jung,Changhoe Kim,Seo Woo Song,Yeongjae Choi,Junho Chung,Byung Gee Kim,Wonshik Han,Sunghoon KwonPubMed 35534484
- eLife 10:2021EKLF/KLF1 expression defines a unique macrophage subset during mouse erythropoiesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaustav Mukherjee,Li Xue,Antanas Planutis,Merlin Nithya Gnanapragasam,Andrew Chess,James J BiekerPubMed 33570494
- PloS one 15:e02303282020A comparison of Chikungunya virus infection, progression, and cytokine profiles in human PMA-differentiated U937 and murine RAW264.7 monocyte derived macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIsrael Guerrero-Arguero et. al.PubMed 32163514
- Journal of proteome research 18:3404-34182019Proteomic Analysis of Merosomes: The Link between Liver and Blood Stages in Malaria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMelanie J Shears,Raja Sekhar Nirujogi,Kristian E Swearingen,Santosh Renuse,Satish Mishra,Panga Jaipal Reddy,Robert L Moritz,Akhilesh Pandey,Photini SinnisPubMed 31335145
- The European journal of neuroscience 33:1598-6102011Hyperphosphorylated Tau in an α-synuclein-overexpressing transgenic model of Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThomas Haggerty et. al.PubMed 21453448
- PloS one 6:e179532011Tauopathic changes in the striatum of A53T α-synuclein mutant mouse model of Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJonathan Wills et. al.PubMed 21445308
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Texas Red® conjugation / labeling in < 20 mins with 30 secs hands-on time using Texas Red® Conjugation Kit (Fast) - Lightning-Link® ab195225. 100% antibody recovery.
Storage
- Shipped at conditions
- Ambient - Cannot Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Texas Red® Conjugation Kit / Texas Red Labeling Kit ab195225 uses a simple and quick process for Texas Red® labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides. Learn about our .To conjugate an antibody to Texas Red® using this kit: - add modifier to antibody and incubate for 15 mins - add quencher and incubate for 5 mins The Texas Red conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid . Use the to learn more about the technology, or about conjugating other proteins and peptides to Texas Red®.Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.
This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link® Rapid Texas Red® Labeling Kit. 315-0005 is the same as the 100 ug size. 315-0010 is the same as the 3 x 100 ug size. 315-0030 is the same as the 3 x 10 ug size. 315-0015 is the same as the 1 mg size.
Amount and volume of antibody for conjugation to Texas Red®
| Kit size | Recommended amount of antibody1 | Maximum amount of antibody | Maximum antibody volume2 |
| 3 x 10 μg | 3 x 10 μg | 3 x 20 μg | 3 x 10 μL |
| 100 μg | 1 x 100 μg | 1 x 200 μg | 1 x 100 μL |
| 3 x 100 μg | 3 x 100 μg | 3 x 200 μg | 3 x 100 μL |
| 1 mg | 1 x 1 mg | 1 x 2 mg | 1 x 1 mL |
1 Using the maximum amount of antibody may result in less labelling per antibody.
2 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 2 mg/ml or < 0.5 mg/ml should be diluted /concentrated.
Buffer Requirements for Conjugation
Buffer should be pH 6.5-8.5.
Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.
| 50mM / 0.6% Tris1 | 0.1% BSA2 | 50% glycerol |
| 0.1% sodium azide | PBS | Potassium phosphate |
| Sodium chloride | HEPES | Sucrose |
| Sodium citrate | EDTA | Trehalose |
1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
2 BSA can also interfere with the use of the conjugated antibody in tissue staining.
Incompatible buffer constituents
| Thiomerosal | Proclin | Glycine |
| Arginine | Glutathione | DTT |
If a constituent of the buffer containing your antibody or protein is not listed above, please contact us.
Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.
Storing and handling conjugation kits
Lyophilized Lightning-Link® components are hygroscopic.
Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Image from Guerrero-Arguero, Israel, et al., PloS one, 15(3): e0230328; doi: 10.1371/journal.pone.0230328. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/Flow Cytometry - Texas Red® Conjugation Kit (Fast) - Lightning-Link® (ab195225)
Guerrero-Arguero, Israel, et al used Texas Red® Conjugation Kit (Fast) - Lightning-Link® (ab195225) as part of examining the rate of productive Chikungunya virus (CHIKV) replication via flow cytometry. They used the kit to conjugate Texas Red® to anti-Chikungunya E1 glycoprotein antibody for use in flow cytometry.
PMA-differentiated U937 and RAW264.7 cells display CHIKV envelope proteins at 6 hpi. PMA = phorbol 12-mystrate 13-acetate
PMA-differentiated U937 and RAW264.7 macrophages were exposed for 2 hours to CHIKV and then fixed and assayed at 8 hpi using flow cytometry and an anti-E1 protein fluorophore-conjugated monoclonal antibody. Data show mean values of three independent experiments with a total of n = 9, MOI = 1. Statistical significance was determined using multiple t-test corrected using Holm-Sidak method. *P<0.05; NS, not significant.
Image from Wills, Jonathan, et al., PLoS One, 6(3): e17953; doi: 10.1371/journal.pone.0017953. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/Immunohistochemistry (PFA perfusion fixed frozen sections) - Texas Red® Conjugation Kit (Fast) - Lightning-Link® (ab195225)
Wills, Jonathan, et al used Texas Red® Conjugation Kit (Fast) - Lightning-Link® (ab195225) as part of examining the aggregation and co-localization of ?-Syn with pGSK-3? and p-Tau in the striatum of A53T α-Syn mutant mice (A53T Tg) and wild-type mice (WT). They used the kit to conjugate Texas Red® to anti-α-Syn antibody for use in immunohistochemistry (PFA perfusion fixed frozen sections).
Right panels constitute merged image of left panels. Sections of striatum of A53T Tg and age-matched WT mice were stained with anti-α-Syn antibody conjugated to Texas Red (red) and anti-p-GSK3β conjugated to FITC (green). Nuclei were stained with DAPI (blue).
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