Cal-520, AM is a fluorogenic calcium-sensitive dye for detecting intracellular calcium mobilization with significantly improved signal-to-noise ratio and intracellular retention compared to existing calcium indicators.
- Molecular Weight – 1102.95 Da
- Provides >100 times fluorescence enhancement upon binding calcium
- Ideal for high-sensitivity calcium mobilization detection
- Suitable for evaluating calcium channels and receptor targets
- Excellent for screening agonists and antagonists in live cell assays
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- Scientific reports 11:100182021Cholinergic calcium responses in cultured antennal lobe neurons of the migratory locust.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGregor A Bergmann,Gerd BickerPubMed 33976252
- Cell death & disease 11:7142020Cannabidiol (CBD): a killer for inflammatory rheumatoid arthritis synovial fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTorsten Lowin et. al.PubMed 32873774
- Glia 68:145-1602019Piezo1 regulates calcium oscillations and cytokine release from astrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaría Velasco-Estevez,Sara O Rolle,Myrthe Mampay,Kumlesh K Dev,Graham K SheridanPubMed 31433095
- Journal of molecular neuroscience : MN 67:16-272018Mitochondrial Electron Transport Chain Complex Dysfunction in MeCP2 Knock-Down Astrocytes: Protective Effects of Quercetin Hydrate.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArpita Dave,Foram Shukla,Hemendra Wala,Prakash PillaiPubMed 30519865
- Cell reports 22:3416-34262018A Conserved Circadian Function for the Neurofibromatosis 1 Gene.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Bai,Yool Lee,Cynthia T Hsu,Julie A Williams,Daniel Cavanaugh,Xiangzhong Zheng,Carly Stein,Paula Haynes,Han Wang,David H Gutmann,Amita SehgalPubMed 29590612
- The Journal of physiology 594:7267-73072016Acetylcholine released by endothelial cells facilitates flow-mediated dilatation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCalum Wilson,Matthew D Lee,John G McCarronPubMed 27730645
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Cal-520, AM is a fluorogenic calcium-sensitive dye for detecting intracellular calcium mobilization with significantly improved signal-to-noise ratio and intracellular retention compared to existing calcium indicators.
- Molecular Weight – 1102.95 Da
- Provides >100 times fluorescence enhancement upon binding calcium
- Ideal for high-sensitivity calcium mobilization detection
- Suitable for evaluating calcium channels and receptor targets
- Excellent for screening agonists and antagonists in live cell assays
Key facts
- Form
- Solid
- Storage buffer
Constituents: 100% Cal-520®, AM
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Store in the dark
Notes
ab171868 provides a robust homogeneous fluorescence-based assay tool for detecting intracellular calcium mobilization. Cal-520 AM is a new fluorogenic calcium-sensitive dye with a significantly improved signal to noise ratio and intracellular retention compared to the existing green calcium indicators (such as Fluo-3 AM and Fluo-4 AM). Cells expressing a GPCR or calcium channel of interest that signals through calcium can be preloaded with Cal-520 AM which can cross cell membrane. Once inside the cell, the lipophilic blocking groups of Cal 520 AM are cleaved by esterases, resulting in a negatively charged fluorescent dye that stays inside cells. Its fluorescence is greatly enhanced upon binding to calcium. When cells stimulated with agonists, the receptor signals the release of intracellular calcium, which significantly increase the fluorescence of Cal-520. The characteristics of its long wavelength, high sensitivity, and >100 times fluorescence enhancement, make Cal-520 AM an ideal indicator for the measurement of cellular calcium. The high S/N ratio and better intracellular retention make the Cal-520 calcium assay a robust tool for evaluating GPCR and calcium channel targets as well as for screening their agonists and antagonists.
Molecular Weight (MW) 1102.95 Da.
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Immunocytochemistry - Cal-520, AM (ab171868)
CHO-M1 cells were seeded overnight at 40,000 cells per 100 μL per well in a 96-well black wall/clear bottom plate. 100 μl of 4 μM Cal 520 AM in HHBS were added into the wells, and the cells were incubated at 37 °C for 2 hour.
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