CFSE - Cell Labeling Kit (ab113853) is a versatile kit for fluorescent intracellular labeling of live cells. CFSE (Carboxyfluorescein succinimidyl ester) enables long-lasting fluorescent labelling of cells, making it ideal for tracking cell division and cell proliferation.
- Non-toxic assay
- Fluorescent label stable even after formaldehyde and alcohol fixation
- Sufficient for ~1000 assays
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- eLife 12:2023Apoptosis recognition receptors regulate skin tissue repair in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOlivia Justynski,Kate Bridges,Will Krause,Maria Fernanda Forni,Quan M Phan,Teresa Sandoval-Schaefer,Kristyn Carter,Diane E King,Henry C Hsia,Michael I Gazes,Steven D Vyce,Ryan R Driskell,Kathryn Miller-Jensen,Valerie HorsleyPubMed 38127424
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- Journal of visualized experiments : JoVE :2022Imaging-Guided Bioreactor for Generating Bioengineered Airway Tissue.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeyed Mohammad Mir,Jiawen Chen,Meghan R Pinezich,John D O'Neill,Brandon A Guenthart,Gordana Vunjak-Novakovic,Jinho KimPubMed 35467661
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- Frontiers in immunology 12:6977252021Intestinal CD11b B Cells Ameliorate Colitis by Secreting Immunoglobulin A.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Fu,Zhiming Wang,Baichao Yu,Yuli Lin,Enyu Huang,Ronghua Liu,Chujun Zhao,Mingfang Lu,Wei Xu,Hongchun Liu,Yongzhong Liu,Luman Wang,Yiwei ChuPubMed 34804004
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Key facts
- Target
- 5(6)-Carboxyfluorescein diacetate succinimidyl ester
- Form
- Liquid
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CFSE - Cell Labeling Kit (ab113853) is a versatile kit for fluorescent intracellular labeling of live cells. CFSE (Carboxyfluorescein succinimidyl ester) enables long-lasting fluorescent labelling of cells, making it ideal for tracking cell division and cell proliferation.
- Non-toxic assay
- Fluorescent label stable even after formaldehyde and alcohol fixation
- Sufficient for ~1000 assays
Key facts
- Target
- 5(6)-Carboxyfluorescein diacetate succinimidyl ester
- Form
- Liquid
- Storage buffer
Constituents: 99% Dimethylsulfoxide, 0.56% 5(6)-Carboxyfluorescein diacetate N-hydroxysuccinimide ester
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
CFSE is a versatile tool for the fluorescent intracellular labeling of live cells. Labeled cells can be assayed using flow cytometry and fluorescent microscopy. The dye is long lasting and well retained within labeled cells. The provided CFSE is sufficient for ~1000 assays.
CFDA-SE (carboxyfluorescein diacetate succinimidyl ester) is a cell permeant molecule and is cleaved by intracellular esterases. The resulting fluorescent molecule CFSE (Carboxyfluorescein succinimidyl ester) indiscriminately with intracellular free amines to generate covalent fluorescent dye-protein conjugates. The result is live cells with an intracellular fluorescent label. At appropriate concentrations CFSE is non-toxic to cells and the fluorescence is retained after formaldehyde and alcohol fixation. CFSE labeled cells can be detected with any instrument or filter set compatible with FITC detection: Excitation(max)=492nm, Emission(max)=517nm.
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3 product images
Flow Cytometry - CFSE - Cell Labeling Kit (ab113853)
Flow cytometry analysis of ab113853 (CFSE) dilution with cell division. Jurkat cells were labeled with 1µM CFSE on day0 and then a portion of the culture was subjected to flow cytometry analysis on days 1-7. Shown, an overlay histogram of the daily CFSE fluorescence intensity.
Flow Cytometry - CFSE - Cell Labeling Kit (ab113853)
Duplexing treated and untreated cells in a single sample. One plate of HeLa cells was labeled with ab113853 (CFSE) and a second plate was treated with the hypoxia mimetic DFO but not labeled with CFSE. After harvesting, the cells were combined in a single tube, stained with a HIF1A antibody and subjected to flow cytometry. Expected results are shown in the left panel cartoon. The no primary antibody control sample (middle panel) shows the resolution of CFSE labeled and unlabeled cells. The HIF1A antibody stained cells (right panel) shows that only cells exposed to DFO have an increase in HIF1A protein levels.
Immunocytochemistry/ Immunofluorescence - CFSE - Cell Labeling Kit (ab113853)
Jurkat cells labeled with ab113853 (CFSE). Jurkat cells were labeled with 1mM CFSE in media for 15 minutes, washed once with PBS and imaged on a flurorescence microscope. The cells in this image are live but fixed cells give similar results.
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