AB113853

CFSE - Cell Labeling Kit

Name: CFSE - Cell Labeling Kit. Live cell labelling. (ab113853) | Abcam
Brand: Abcam Limited
SKU: AB113853
Price: 370 USD
Availability: InStock

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(36 Publications)

CFSE - Cell Labeling Kit (ab113853) is a versatile kit for fluorescent intracellular labeling of live cells. CFSE (Carboxyfluorescein succinimidyl ester) enables long-lasting fluorescent labelling of cells, making it ideal for tracking cell division and cell proliferation.

- Non-toxic assay
- Fluorescent label stable even after formaldehyde and alcohol fixation
- Sufficient for ~1000 assays

3 Images

Flow Cytometry - CFSE - Cell Labeling Kit (AB113853)

Flow Cyt

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Flow Cytometry - CFSE - Cell Labeling Kit (AB113853)

Flow cytometry analysis of ab113853 (CFSE) dilution with cell division. Jurkat cells were labeled with 1µM CFSE on day0 and then a portion of the culture was subjected to flow cytometry analysis on days 1-7. Shown, an overlay histogram of the daily CFSE fluorescence intensity.

Immunocytochemistry/ Immunofluorescence - CFSE - Cell Labeling Kit (AB113853)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - CFSE - Cell Labeling Kit (AB113853)

Jurkat cells labeled with ab113853 (CFSE). Jurkat cells were labeled with 1mM CFSE in media for 15 minutes, washed once with PBS and imaged on a flurorescence microscope. The cells in this image are live but fixed cells give similar results.

Flow Cyt

Supplier Data

Flow Cytometry - CFSE - Cell Labeling Kit (AB113853)

Duplexing treated and untreated cells in a single sample. One plate of HeLa cells was labeled with ab113853 (CFSE) and a second plate was treated with the hypoxia mimetic DFO but not labeled with CFSE. After harvesting, the cells were combined in a single tube, stained with a HIF1A antibody and subjected to flow cytometry. Expected results are shown in the left panel cartoon. The no primary antibody control sample (middle panel) shows the resolution of CFSE labeled and unlabeled cells. The HIF1A antibody stained cells (right panel) shows that only cells exposed to DFO have an increase in HIF1A protein levels.

Key facts

Target

5(6)-Carboxyfluorescein diacetate succinimidyl ester

target

Form

Liquid

form

Storage buffer

Constituents: 99% Dimethylsulfoxide, 0.56% 5(6)-Carboxyfluorescein diacetate N-hydroxysuccinimide ester

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Product details

CFSE is a versatile tool for the fluorescent intracellular labeling of live cells. Labeled cells can be assayed using flow cytometry and fluorescent microscopy. The dye is long lasting and well retained within labeled cells. The provided CFSE is sufficient for ~1000 assays.

CFDA-SE (carboxyfluorescein diacetate succinimidyl ester) is a cell permeant molecule and is cleaved by intracellular esterases. The resulting fluorescent molecule CFSE (Carboxyfluorescein succinimidyl ester) indiscriminately with intracellular free amines to generate covalent fluorescent dye-protein conjugates. The result is live cells with an intracellular fluorescent label. At appropriate concentrations CFSE is non-toxic to cells and the fluorescence is retained after formaldehyde and alcohol fixation. CFSE labeled cells can be detected with any instrument or filter set compatible with FITC detection: Excitation(max)=492nm, Emission(max)=517nm.

Cell proliferation assays are indispensable for advancing scientific discovery and clinical applications. Browse our comprehensive selection to support your research.

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Product protocols

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Target data

See full target information 5(6)-Carboxyfluorescein diacetate succinimidyl ester

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Publications (36)

Recent publications for all applications. Explore the full list and refine your search

Cancer immunology, immunotherapy : CII 74:226 PubMed40445421

2025

Stabilized iRGD modification enhances NY-ESO-1 TCR-T infiltration in solid tumors and synergizes with PD-1 blockade.

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Yirong Wu,Lanqun Qin,Jiayu Wang,Ziyao Xie,Xinyu Su,Xiang Li,Yueling Yang,Rong Huang,Mengke Zhao,Lianjun Zhao,Zhengyun Zou

Journal of nanobiotechnology 22:638 PubMed39420389

2024

Novel combination therapy using recombinant oncolytic adenovirus silk hydrogel and PD-L1 inhibitor for bladder cancer treatment.

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Wenqiang Zhang,Jianqiang Zhang,Jingwei Zhang,Jing Chu,Zhenxing Zhang

Histology and histopathology 40:1061-1071 PubMed39410869

2024

DNMT1 silencing induces KIR2DL1/2/3 expression via methylation to alleviate graft--host disease after allogeneic hematopoietic stem cell transplantation.

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Ping Zhang,Shuling Yu,Miao Zhou,Xiao Yan,Huiling Zhu,Lixia Sheng,Yi Zhang,Shujun Yang,Guifang Ouyang

Journal of clinical medicine 13: PubMed39336917

2024

Molecular and Functional Cargo of Plasma-Derived Exosomes in Patients with Hereditary Hemorrhagic Telangiectasia.

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Yanru Wang,Linda Hofmann,Diana Huber,Robin Lochbaum,Sonja Ludwig,Cornelia Brunner,Thomas K Hoffmann,René Lehner,Marie-Nicole Theodoraki

Science advances 10:eadq1476 PubMed39292787

2024

CCR2 cooperativity promotes hematopoietic stem cell homing to the bone marrow.

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Stephanie N Hurwitz,Danielle R Kobulsky,Seul K Jung,Jennifer J Chia,Jason M Butler,Peter Kurre

eLife 12: PubMed38127424

2023

Apoptosis recognition receptors regulate skin tissue repair in mice.

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Olivia Justynski,Kate Bridges,Will Krause,Maria Fernanda Forni,Quan M Phan,Teresa Sandoval-Schaefer,Kristyn Carter,Diane E King,Henry C Hsia,Michael I Gazes,Steven D Vyce,Ryan R Driskell,Kathryn Miller-Jensen,Valerie Horsley

Cell reports 42:113448 PubMed37967010

2023

T cell-specific P2RX7 favors lung parenchymal CD4 T cell accumulation in response to severe lung infections.

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Igor Santiago-Carvalho,Gislane Almeida-Santos,Bruna Gois Macedo,Caio Cesar Barbosa-Bomfim,Fabricio Moreira Almeida,Marcos Vinícios Pinheiro Cione,Trupti Vardam-Kaur,Mia Masuda,Sarah Van Dijk,Bruno Marcel Melo,Rogério Silva do Nascimento,Rebeka da Conceição Souza,Alba Lucínia Peixoto-Rangel,Robson Coutinho-Silva,Mario Hiroyuki Hirata,José Carlos Alves-Filho,José Maria Álvarez,Elena Lassounskaia,Henrique Borges da Silva,Maria Regina D'Império-Lima

Stem cell research & therapy 14:208 PubMed37605275

2023

Substrate topographies modulate the secretory activity of human bone marrow mesenchymal stem cells.

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Heizel Rosado-Galindo,Maribella Domenech

3 Biotech 13:283 PubMed37501919

2023

A novel monospecific tetravalent IgG1-(scFv) version shown enhanced neutralizing and Fc-mediated effector functions against SARS-CoV-2.

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Zhang-Zhao Gao,Jing-Yu Jiao,Ya-Qiong Zhou,Jian Qi,Shan-Shan Zhu,Jing-Ya Xu,Lei Nie,Hai-Bin Wang

Journal of the American Society of Nephrology : JASN 34:809-828 PubMed36724799

2023

Hyperactivation of YAP/TAZ Drives Alterations in Mesangial Cells through Stabilization of N-Myc in Diabetic Nephropathy.

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Seunghyeok Choi,Seon Pyo Hong,Jung Hyun Bae,Sang Heon Suh,Hosung Bae,Kyung Pyo Kang,Hyuek Jong Lee,Gou Young Koh

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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CFSE - Cell Labeling Kit

Flow Cytometry - CFSE - Cell Labeling Kit (AB113853)

Immunocytochemistry/ Immunofluorescence - CFSE - Cell Labeling Kit (AB113853)

Flow Cytometry - CFSE - Cell Labeling Kit (AB113853)

Key facts

Target

Form

Storage buffer

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product details

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (36)

Product promise