CyTRAK Orange™ is a novel orange fluorescent dye [related to DRAQ5™ (ab108410)] that stains both nucleus and cytoplasm. It is water soluble and membrane permeant which can be used in live and fixed cells in combination with other common fluorophores, such as GFP-fusions, green-labelled antibodies (FITC or DyLight^® 488) and far-red dyes. CyTRAK Orange™ preferentially stains the nucleus, but also defines the cytoplasmic area in both live and fixed preparations. CyTRAK Orange™ does the work of two fluorophores! Key features of CyTRAK Orange™ include:

Rapid staining of dsDNA/nuclei of LIVE or fixed cells
Differential intensity of DNA binding and Cytoplasmic labelling - One fluorophore doing the work of two
Low photobleaching effect
Ideal for use with GFP, FITC & DyLight^® 488 and 649 labels
No lyse / no wash protocols
It is membrane-permeant

Excitation

457, 488 & 549 (Ex_max 515nm)
similar profile to Propidium Iodide
co-excited with FITC/Cy2/eGFP and PE-Cy7
NOT excited by UV or red lasers (e.g. 633 & 647 nm)

Emission (instrument dependent):

Em_max 615 nm
No significant spectral overlap with co-excited green fluors (eGFP, FITC, Cy2)
Visualize GFP-tagged temporospatial changes in live cells in a single acquisition from a 488 nm excitation

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CyTRAK Orange™ 50µl (5 mM) (ab109203) is unavailable

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