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DRAQ5™ is a cell-permeable, far-red fluorescent DNA dye that can be used for labeling DNA in both live/non-fixed and fixed cells, compatible with common fluorescent labels like GFP or FITC.


- Cited in over 50 publications
- Far-red fluorescence – excitation at 647 nm and emission at 665 nm, with minimal overlap with GFP and FITC
- No Photobleaching – provides durable staining without fading
- Compatible with multiple applications such as immunocytochemistry/immunofluorescence, flow cytometry, and fluorescence microscopy

Images

Immunocytochemistry/ Immunofluorescence - DRAQ5™ (AB108410), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - DRAQ5™ (AB108410), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - DRAQ5™ (AB108410), expandable thumbnail

Publications

Key facts

Applications
ICC/IF, Flow Cyt, Fluorescence Microscopy
Target
DRAQ5
Form
Liquid

Reactivity data

Application
ICC/IF
Reactivity
Reacts
Dilution info
1/1000
Notes

For Cell-based assays, Immunofluorescence microscopy and In-Cell WB = 5µM. It is highly recommended that the concentration and labelling conditions are carefully determined by each investigator for optimal performance in the assay of interest. For more specific information about the applications, please refer to the Protocol Booklet section.

Application
Flow Cyt
Reactivity
Reacts
Dilution info
1/250.00000 - 1/1000.00000
Notes

For Gating of Nucleated cells = 5µM; For Cell Cycle Analysis = 20µM

Application
Fluorescence Microscopy
Reactivity
Reacts
Dilution info
-
Notes

-

Recommended products

DRAQ5™ is a cell-permeable, far-red fluorescent DNA dye that can be used for labeling DNA in both live/non-fixed and fixed cells, compatible with common fluorescent labels like GFP or FITC.


- Cited in over 50 publications
- Far-red fluorescence – excitation at 647 nm and emission at 665 nm, with minimal overlap with GFP and FITC
- No Photobleaching – provides durable staining without fading
- Compatible with multiple applications such as immunocytochemistry/immunofluorescence, flow cytometry, and fluorescence microscopy

Key facts

Applications
ICC/IF, Flow Cyt, Fluorescence Microscopy
Target
DRAQ5
Form
Liquid
Storage buffer

Constituents: 100% 1,5-BIS{[2- (DIMETHYLAMINO) ETHYL]AMINO}-4,8- DIHYDROXYANTHRACE NE-9,10-DIONE

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze, Store in the dark

Notes

DRAQ5™ is a cell permeable far-red fluorescent DNA dye that can be used in fixed or non-fixed/ live cells in combination with common labels such as GFP or FITC.

As with any cell­-permeant DNA intercalating probe, DRAQ5 may inhibit cell division in long-term assays and should be tested for any effect.

DRAQ5 staining can be used in flow cytometry, live cell imaging and cell-based assays and the dye is highly compatible with standard protocols across many instrumentation platforms.

The chemical name of DRAQ5 is 1, 5-bis{[2-(di-methylamino)ethyl]amino}-4, 8-dihydroxyanthracene-9, 10-dione.

The advantages of DRAQ5 staining include
- convenient ready-to-use aqueous solution
- rapid uptake into living cells, providing a high level of nuclear discrimination
- no photobleaching effect
- can be used in most cell types, eukaryotic and prokaryotic: mammalian, bacterial, parasitic, plant, etc.
- no compensation needed with common FITC/GFP + PE combinations in flow cytometry
- no RNase treatment required
- no fluorescence enhancement upon DNA binding
- compatible with optics of benchtop flow, laser scanning cytometers and non-UV laser scanning and lamp-based confocal microscopes

SPECTRAL PROPERTIES:

Excitation

  • 647 nm line optimal (Exmax 646 nm)
  • 488, 514, 568 and 633 nm lines, sub-optimal
  • Two-photon excitation (1047 nm) and excitation dark (700-850 nm)

Emission (instrument dependent):
- 665 nm to infra-red max 681 nm / 697 nm intercalated with dsDNA)
- minimal overlap with vis range e.g. GFP and FITC
- Em. filters may include 695L, 715LP or 780 LP

Concentration: 5 mM

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com