DRAQ5™ is a cell-permeable, far-red fluorescent DNA dye that can be used for labeling DNA in both live/non-fixed and fixed cells, compatible with common fluorescent labels like GFP or FITC.
- Cited in over 50 publications
- Far-red fluorescence – excitation at 647 nm and emission at 665 nm, with minimal overlap with GFP and FITC
- No Photobleaching – provides durable staining without fading
- Compatible with multiple applications such as immunocytochemistry/immunofluorescence, flow cytometry, and fluorescence microscopy
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Publications
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- The EMBO journal 40:e1065032021The TBC1D31/praja2 complex controls primary ciliogenesis through PKA-directed OFD1 ubiquitylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmanuela Senatore,Francesco Chiuso,Laura Rinaldi,Daniela Intartaglia,Rossella Delle Donne,Emilia Pedone,Bruno Catalanotti,Luciano Pirone,Bianca Fiorillo,Federica Moraca,Giuliana Giamundo,Giovanni Scala,Andrea Raffeiner,Omar Torres-Quesada,Eduard Stefan,Marcel Kwiatkowski,Alienke van Pijkeren,Manuela Morleo,Brunella Franco,Corrado Garbi,Ivan Conte,Antonio FelicielloPubMed 33934390
- Molecular systems biology 17:e102322021Single-cell analyses reveal SARS-CoV-2 interference with intrinsic immune response in the human gut.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSergio Triana,Camila Metz-Zumaran,Carlos Ramirez,Carmon Kee,Patricio Doldan,Mohammed Shahraz,Daniel Schraivogel,Andreas R Gschwind,Ashwini K Sharma,Lars M Steinmetz,Carl Herrmann,Theodore Alexandrov,Steeve Boulant,Megan L StaniferPubMed 33904651
- Frontiers in immunology 12:6599962021Triple-Negative Breast Cancer Cells Recruit Neutrophils by Secreting TGF-β and CXCR2 Ligands.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuvasree SenGupta,Lauren E Hein,Yang Xu,Jason Zhang,Jamie R Konwerski,Ye Li,Craig Johnson,Dawen Cai,Janet L Smith,Carole A ParentPubMed 33912188
- JCI insight 6:2021Deep learning-based molecular morphometrics for kidney biopsies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarina Zimmermann,Martin Klaus,Milagros N Wong,Ann-Katrin Thebille,Lukas Gernhold,Christoph Kuppe,Maurice Halder,Jennifer Kranz,Nicola Wanner,Fabian Braun,Sonia Wulf,Thorsten Wiech,Ulf Panzer,Christian F Krebs,Elion Hoxha,Rafael Kramann,Tobias B Huber,Stefan Bonn,Victor G PuellesPubMed 33705360
- PloS one 16:e02481392021Development of a novel in vitro assay to screen for neuroprotective drugs against iatrogenic neurite shortening.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAntón L Martínez,José Brea,Mateo Barro,Xavier Monroy,Manuel Merlos,Javier Burgueño,María Isabel LozaPubMed 33690613
- Nature communications 12:11602021Neuronal fragile X mental retardation protein activates glial insulin receptor mediated PDF-Tri neuron developmental clearance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDominic J Vita,Cole J Meier,Kendal BroadiePubMed 33608547
- Current protocols 1:e512021Human Plasma In-Cell Western Assays-An In vitro Predictor for In vivo Pharmacology in Oncology Drug Discovery.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang W Zhang,Kirsten Gallagher,Dimitrios Angelis,Dave Rominger,Peggy Scherle,Kris VaddiPubMed 33587334
- Nature 590:115-1212020Bidirectional perisomatic inhibitory plasticity of a Fos neuronal network.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEe-Lynn Yap,Noah L Pettit,Christopher P Davis,M Aurel Nagy,David A Harmin,Emily Golden,Onur Dagliyan,Cindy Lin,Stephanie Rudolph,Nikhil Sharma,Eric C Griffith,Christopher D Harvey,Michael E GreenbergPubMed 33299180
- Nucleic acids research 48:12085-121012020Benzo[a]pyrene represses DNA repair through altered E2F1/E2F4 function marking an early event in DNA damage-induced cellular senescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSebastian Allmann,Laura Mayer,Jessika Olma,Bernd Kaina,Thomas G Hofmann,Maja T Tomicic,Markus ChristmannPubMed 33166399
- Cell 183:1058-1069.e192020A Therapeutic Non-self-reactive SARS-CoV-2 Antibody Protects from Lung Pathology in a COVID-19 Hamster Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJakob Kreye et. al.PubMed 33058755
Key facts
- Applications
- ICC/IF, Flow Cyt, Fluorescence Microscopy
- Target
- DRAQ5
- Form
- Liquid
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ICC/IF | Reactivity Reacts | Dilution info 1/1000 | Notes For Cell-based assays, Immunofluorescence microscopy and In-Cell WB = 5µM. It is highly recommended that the concentration and labelling conditions are carefully determined by each investigator for optimal performance in the assay of interest. For more specific information about the applications, please refer to the Protocol Booklet section. |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/250.00000 - 1/1000.00000 | Notes For Gating of Nucleated cells = 5µM; For Cell Cycle Analysis = 20µM |
Application Fluorescence Microscopy | Reactivity Reacts | Dilution info - | Notes - |
Target data
Recommended products
DRAQ5™ is a cell-permeable, far-red fluorescent DNA dye that can be used for labeling DNA in both live/non-fixed and fixed cells, compatible with common fluorescent labels like GFP or FITC.
- Cited in over 50 publications
- Far-red fluorescence – excitation at 647 nm and emission at 665 nm, with minimal overlap with GFP and FITC
- No Photobleaching – provides durable staining without fading
- Compatible with multiple applications such as immunocytochemistry/immunofluorescence, flow cytometry, and fluorescence microscopy
Key facts
- Applications
- ICC/IF, Flow Cyt, Fluorescence Microscopy
- Target
- DRAQ5
- Form
- Liquid
- Storage buffer
Constituents: 100% 1,5-BIS{[2- (DIMETHYLAMINO) ETHYL]AMINO}-4,8- DIHYDROXYANTHRACE NE-9,10-DIONE
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze, Store in the dark
Notes
DRAQ5™ is a cell permeable far-red fluorescent DNA dye that can be used in fixed or non-fixed/ live cells in combination with common labels such as GFP or FITC.
As with any cell-permeant DNA intercalating probe, DRAQ5 may inhibit cell division in long-term assays and should be tested for any effect.
DRAQ5 staining can be used in flow cytometry, live cell imaging and cell-based assays and the dye is highly compatible with standard protocols across many instrumentation platforms.
The chemical name of DRAQ5 is 1, 5-bis{[2-(di-methylamino)ethyl]amino}-4, 8-dihydroxyanthracene-9, 10-dione.
The advantages of DRAQ5 staining include
- convenient ready-to-use aqueous solution
- rapid uptake into living cells, providing a high level of nuclear discrimination
- no photobleaching effect
- can be used in most cell types, eukaryotic and prokaryotic: mammalian, bacterial, parasitic, plant, etc.
- no compensation needed with common FITC/GFP + PE combinations in flow cytometry
- no RNase treatment required
- no fluorescence enhancement upon DNA binding
- compatible with optics of benchtop flow, laser scanning cytometers and non-UV laser scanning and lamp-based confocal microscopes
SPECTRAL PROPERTIES:
Excitation
- 647 nm line optimal (Exmax 646 nm)
- 488, 514, 568 and 633 nm lines, sub-optimal
- Two-photon excitation (1047 nm) and excitation dark (700-850 nm)
Emission (instrument dependent):
- 665 nm to infra-red max 681 nm / 697 nm intercalated with dsDNA)
- minimal overlap with vis range e.g. GFP and FITC
- Em. filters may include 695L, 715LP or 780 LP
Concentration: 5 mM
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Immunocytochemistry/ Immunofluorescence - DRAQ5™ (ab108410)
HeLa cells were stained with Lamin B1 antibody - Nuclear Envelope Marker (Anti-Lamin B1 antibody - Nuclear Envelope Marker ab16048) and alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291). The cells were 100% methanol fixed (5 min) and then incubated in 1% BSA in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the primary antibodies (Anti-Lamin B1 antibody - Nuclear Envelope Marker ab16048 & Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1μg/ml overnight at 4C. The secondary antibodies were Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) (green) and Goat polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 594), pre-adsorbed (Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed ab96881) (red) used at 1/250 dilution for 1h at room temperature. 5μM DRAQ5 was added to the secondary antibody mixture to label nuclear DNA (pseudocolor purple).
Immunocytochemistry/ Immunofluorescence - DRAQ5™ (ab108410)
HeLa cells were stained with beta Catenin antibody (Anti-beta Catenin antibody ab16051) and alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291). The cells were 100% methanol fixed (5 min) and then incubated in 1% BSA in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the primary antibodies (Anti-beta Catenin antibody ab16051 & ab7921) at 1μg/ml overnight at 4C. The secondary antibodies were Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) (green) and Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 594), pre-adsorbed (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) (red) used at 1/250 dilution for 1h at room temperature. 5μM DRAQ5 was added to the secondary antibody mixture to label nuclear DNA (pseudocolor orange).
Immunocytochemistry/ Immunofluorescence - DRAQ5™ (ab108410)
DRAQ5™-stained nuclei in a adult Drosophila brain.
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