DRAQ7™

DRAQ7™ is a far-red fluorescent dye that only stains the nuclei in dead and permeabilized cells and can be used in combination with common labels such as GFP or FITC.

DRAQ7 is the ideal tool to study dead or membrane-compromised cells because it does not enter intact, live cells. It is an ideal replacement for propidium iodide and 7-AAD, as is not excited by UV light and has no emission overlap with PE / PE homologues.

Key features of DRAQ7 include:

• Rapid staining of dsDNA/ nuclei of dead or permeabilized cells
• Low photobleaching
• It can be used in most cell types, eukaryotic and prokaryotic: mammalian, bacterial, parasitic, plant, etc ...
• Non-toxic in long-term culture
• Can be combined with "live" dyes
• No compensation needed with common FITC/GFP + PE combinations in flow cytometry
• No wash or RNase treatment needed.

SPECTRAL PROPERTIES

Excitation:

• 633 and 647 nm line optimal (Ex_max 599 / 644 nm)
• 488, 514 and 568 nm lines, sub-optimal (only by flow cytometry)

Emission (instrument dependent):

• 665 nm to infra-red max 678 nm / 697 nm intercalated with dsDNA)
• Minimal overlap with vis range e.g. GFP and FITC
• Em. filters may include 695L, 715LP or 780 LP

Multi-wavelength imaging with UV / vis fluorochromes

• No fluorescence enhancement upon DNA binding
• Low photo-bleaching effect
• Compatible with optics of flow, laser scanning cytometers and confocal and lamp-based fluoroscence microscopes