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AB109202

DRAQ7™

5

(1 Review)

|

(88 Publications)

DRAQ7™ (ab109202) is a far-red fluorescent dye that only stains the nuclei of dead and permeabilized cells, making it ideal for cell viability studies. Suitable for Flow cytometry, ICC/IF, IHC and other fluorescent applications. Used in combination with GFP or FITC labels.

- Rapid staining of doubles-stranded (ds) DNA/ nuclei of dead or permeabilized cells
- Low photobleaching effect
- No RNase treatment required
- Suitable for eukaryotic and prokaryotic cell types including mammalian, bacterial, parasitic, plant.
3 Images
Immunohistochemistry (Frozen sections) - DRAQ7™ (AB109202)
  • IHC-Fr

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Immunohistochemistry (Frozen sections) - DRAQ7™ (AB109202)

Sample : mouse embryonic stem cell-differentiated embryoid bodies (EBs)

Preparation :

  • Fix in 3%PFA in PBS for 30 min at RT
  • Incubate in 7.5% sucrose-PBS for 3h at RT
  • Incubate in 15% sucrose-PBS at 4°C overnight
  • Embed the EBs in tissue-Tek OCT compound
  • Cut frozen sections to 4-20 μm thickness

Primary antibody : Rabbit anti-laminin alpha 1, 1 : 400
Secondary antibody : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) (ab97050), 1 : 200
F-actin was stained with CytoPainter F-actin staining kit (blue) (ab112124), 1 : 1000
Nuclei were counterstained stained with DRAQ7™, 1 : 1000

Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School

Flow Cytometry - DRAQ7™ (AB109202)
  • Flow Cyt

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Flow Cytometry - DRAQ7™ (AB109202)

Jurkat cells exposed to 1μM staurosporine for 24 hours show DRAQ7™ staining (top half of the plot). These cells have compromised membranes that allow entry of DRAQ7™ in the cells.

Immunohistochemistry (Frozen sections) - DRAQ7™ (AB109202)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - DRAQ7™ (AB109202)

Sample : mouse embryonic stem cell-differentiated embryoid bodies (EBs)
Preparation :

  • Fix in 3%PFA in PBS for 30 min at RT
  • Incubate in 7.5% sucrose-PBS for 3h at RT
  • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
  • Embed the EBs in tissue-Tek OCT compound
  • Cut frozen sections to 4-20 μm thickness

Primary antibody : Rabbit anti-laminin alpha 1, 1 : 400
Secondary antibody : Goat anti-rabbit IgG - H&L (AMCA) (ab123435)
Nuclei were counterstained stained with DRAQ7™ (ab109202)

Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School

Key facts

Applications

Fluorescence Microscopy, ICC/IF, Flow Cyt

applications

Target

DRAQ7

target

Form

Liquid

form

Storage buffer

Constituents: 1% Proprietary derivative of 1,5-BIS{[2-(DIMETHYLAMINO) ETHYL]AMINO}-4,8-DIHYDROXYANTHRACENE-9,10-DIONE

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100", "notes":"<p>(final concentration = 3µM). It is highly recommended that the concentration and labelling conditions are carefully determined by each investigator for optimal performance in the assay of interest.For more specific information about the applications, please refer to the Protocol Booklet.</p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100", "notes":"<p>(final concentration = 3µM)</p>" }, "Fluorescence Microscopy": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

DRAQ7™ is a far-red fluorescent dye that only stains the nuclei in dead and permeabilized cells and can be used in combination with common labels such as GFP or FITC.

DRAQ7 is the ideal tool to study dead or membrane-compromised cells because it does not enter intact, live cells. It is an ideal replacement for propidium iodide and 7-AAD, as is not excited by UV light and has no emission overlap with PE / PE homologues.

Key features of DRAQ7 include:

  • Rapid staining of dsDNA/ nuclei of dead or permeabilized cells
  • Low photobleaching
  • It can be used in most cell types, eukaryotic and prokaryotic: mammalian, bacterial, parasitic, plant, etc ...
  • Non-toxic in long-term culture
  • Can be combined with "live" dyes
  • No compensation needed with common FITC/GFP + PE combinations in flow cytometry
  • No wash or RNase treatment needed.

SPECTRAL PROPERTIES

Excitation:

  • 633 and 647 nm line optimal (Exmax 599 / 644 nm)
  • 488, 514 and 568 nm lines, sub-optimal (only by flow cytometry)

Emission (instrument dependent):

  • 665 nm to infra-red max 678 nm / 697 nm intercalated with dsDNA)
  • Minimal overlap with vis range e.g. GFP and FITC
  • Em. filters may include 695L, 715LP or 780 LP

Multi-wavelength imaging with UV / vis fluorochromes

  • No fluorescence enhancement upon DNA binding
  • Low photo-bleaching effect
  • Compatible with optics of flow, laser scanning cytometers and confocal and lamp-based fluoroscence microscopes
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information DRAQ7
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Publications (88)

Recent publications for all applications. Explore the full list and refine your search

Endocrinology 166: PubMed40294160

2025

CCAAT/Enhancer-Binding Proteins α and β Regulate Ovulation and Gene Expression via Dose- and Stage-Dependent Mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Hanxue Zhang,Rainer B Lanz,Jimmy Dhillon,Paul D Soloway,Bo Shui,Yi Athena Ren

Respiratory research 26:124 PubMed40176049

2025

Heme-induced lung injury in human precision cut lung slices: a new model for acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Namrata Kewalramani,Carlos Machahua,Thomas Michael Marti,Cas Zandbergen,Savvina Chortarea,Jessica Beretta-Piccoli,Christophe von Garnier,Patrick Dorn,Kleanthis Fytianos,Manuela Funke-Chambour

Cell reports 44:115259 PubMed39893637

2025

The homologous recombination factors BRCA2 and PALB2 interplay with mismatch repair pathways to maintain centromere stability and cell viability.

Applications

Unspecified application

Species

Unspecified reactive species

Emily Graham,Lucia Rampazzo,Chin Wei Brian Leung,Jacob Wall,Emőke Zsanett Gerőcz,Mikhail Liskovykh,Nikolay Goncharov,Xanita Saayman,Ramazan Gundogdu,Masato T Kanemaki,Hiroshi Masumoto,Vladimir Larionov,Natalay Kouprina,Fumiko Esashi

NPJ systems biology and applications 11:12 PubMed39875420

2025

Merging metabolic modeling and imaging for screening therapeutic targets in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Niki Tavakoli,Emma J Fong,Abigail Coleman,Yu-Kai Huang,Mathias Bigger,Michael E Doche,Seungil Kim,Heinz-Josef Lenz,Nicholas A Graham,Paul Macklin,Stacey D Finley,Shannon M Mumenthaler

Nature protocols 20:810-842 PubMed39501108

2024

Life-long functional regeneration of in vivo airway epithelium by the engraftment of airway basal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Ma,Bibek R Thapa,Jake A Le Suer,Andrew Tilston-Lünel,Michael J Herriges,Feiya Wang,Pushpinder S Bawa,Xaralabos Varelas,Finn J Hawkins,Darrell N Kotton

The EMBO journal 43:4110-4135 PubMed38997504

2024

PUFFFIN: an ultra-bright, customisable, single-plasmid system for labelling cell neighbourhoods.

Applications

Unspecified application

Species

Unspecified reactive species

Tamina Lebek,Mattias Malaguti,Giulia Lm Boezio,Lida Zoupi,James Briscoe,Alistair Elfick,Sally Lowell

iScience 27:110169 PubMed38993668

2024

A specialized population of monocyte-derived tracheal macrophages promote airway epithelial regeneration through a CCR2-dependent mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra B Ysasi,Anna E Engler,Pushpinder Singh Bawa,Feiya Wang,Regan D Conrad,Anthony K Yeung,Jason R Rock,Jennifer Beane-Ebel,Sarah A Mazzilli,Ruth A Franklin,Joseph P Mizgerd,George J Murphy

Frontiers in immunology 15:1427100 PubMed38983847

2024

An antibody to IL-1 receptor 7 protects mice from LPS-induced tissue and systemic inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Liqiong Jiang,Lars P Lunding,William S Webber,Karsten Beckmann,Tania Azam,Jesper Falkesgaard Højen,Jesus Amo-Aparicio,Alberto Dinarello,Tom T Nguyen,Ulrich Pessara,Daniel Parera,David J Orlicky,Stephan Fischer,Michael Wegmann,Charles A Dinarello,Suzhao Li

eLife 13: PubMed38874379

2024

A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Haley R Noonan,Alexandra M Thornock,Julia Barbano,Michael E Xifaras,Chloe S Baron,Song Yang,Katherine Koczirka,Alicia M McConnell,Leonard I Zon

Nature 631:170-178 PubMed38768632

2024

In vitro reconstitution of epigenetic reprogramming in the human germ line.

Applications

Unspecified application

Species

Unspecified reactive species

Yusuke Murase,Ryuta Yokogawa,Yukihiro Yabuta,Masahiro Nagano,Yoshitaka Katou,Manami Mizuyama,Ayaka Kitamura,Pimpitcha Puangsricharoen,Chika Yamashiro,Bo Hu,Ken Mizuta,Taro Tsujimura,Takuya Yamamoto,Kosuke Ogata,Yasushi Ishihama,Mitinori Saitou
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com