DRAQ7™ is a far-red fluorescent dye that only stains the nuclei in dead and permeabilized cells and can be used in combination with common labels such as GFP or FITC.
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- Nucleic acids research 49:9007-90252021RNA-regulatory exosome complex confers cellular survival to promote erythropoiesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCharu Mehta,Isabela Fraga de Andrade,Daniel R Matson,Colin N Dewey,Emery H BresnickPubMed 34059908
- Cancers 12:2020Modulation of de Novo Lipogenesis Improves Response to Enzalutamide Treatment in Prostate Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMohamed Amine Lounis et. al.PubMed 33187317
- Angiogenesis 23:567-5752020Excess centrosomes disrupt vascular lumenization and endothelial cell adherens junctions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDanielle B Buglak et. al.PubMed 32699963
- Scientific reports 10:120232020Immune lymphocytes halt replication of Francisella tularensis LVS within the cytoplasm of infected macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMary Katherine Bradford et. al.PubMed 32694562
- Cells 9:2020DNA Damage- But Not Enzalutamide-Induced Senescence in Prostate Cancer Promotes Senolytic Bcl-xL Inhibitor Sensitivity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNicolas Malaquin et. al.PubMed 32630281
- eLife 9:2020Apolipoprotein L-1 renal risk variants form active channels at the plasma membrane driving cytotoxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoseph A Giovinazzo et. al.PubMed 32427098
- SLAS discovery : advancing life sciences R & D 25:744-7542020Comparison of Cell and Organoid-Level Analysis of Patient-Derived 3D Organoids to Evaluate Tumor Cell Growth Dynamics and Drug Response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeungil Kim,Sarah Choung,Ren X Sun,Nolan Ung,Natasha Hashemi,Emma J Fong,Roy Lau,Erin Spiller,Jordan Gasho,Jasmine Foo,Shannon M MumenthalerPubMed 32349587
- Nature communications 11:13932020Amino acid levels determine metabolism and CYP450 function of hepatocytes and hepatoma cell lines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuben Boon et. al.PubMed 32170132
- Frontiers in oncology 9:13112019Targeting of the Eukaryotic Translation Initiation Factor 4A Against Breast Cancer Stemness.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSangita Sridharan et. al.PubMed 31867270
- Experimental eye research 189:1078172019Blue-light induced breakdown of barrier function on human retinal epithelial cells is mediated by PKC-ζ over-activation and oxidative stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEge Kaan Ozkaya et. al.PubMed 31563609
Key facts
- Applications
- ICC/IF, Flow Cyt, Fluorescence Microscopy
- Target
- DRAQ7
- Form
- Liquid
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ICC/IF | Reactivity Reacts | Dilution info 1/100 | Notes (final concentration = 3µM). It is highly recommended that the concentration and labelling conditions are carefully determined by each investigator for optimal performance in the assay of interest.For more specific information about the applications, please refer to the Protocol Booklet. |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/100 | Notes (final concentration = 3µM) |
Application Fluorescence Microscopy | Reactivity Reacts | Dilution info - | Notes - |
Target data
Recommended products
DRAQ7™ is a far-red fluorescent dye that only stains the nuclei in dead and permeabilized cells and can be used in combination with common labels such as GFP or FITC.
Key facts
- Applications
- ICC/IF, Flow Cyt, Fluorescence Microscopy
- Target
- DRAQ7
- Form
- Liquid
- Storage buffer
Constituents: 1% Proprietary derivative of 1,5-BIS{[2-(DIMETHYLAMINO) ETHYL]AMINO}-4,8-DIHYDROXYANTHRACENE-9,10-DIONE
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze, Store in the dark
Notes
DRAQ7™ is a far-red fluorescent dye that only stains the nuclei in dead and permeabilized cells and can be used in combination with common labels such as GFP or FITC.
DRAQ7 is the ideal tool to study dead or membrane-compromised cells because it does not enter intact, live cells. It is an ideal replacement for propidium iodide and 7-AAD, as is not excited by UV light and has no emission overlap with PE / PE homologues.
Key features of DRAQ7 include:
- Rapid staining of dsDNA/ nuclei of dead or permeabilized cells
- Low photobleaching
- It can be used in most cell types, eukaryotic and prokaryotic: mammalian, bacterial, parasitic, plant, etc ...
- Non-toxic in long-term culture
- Can be combined with "live" dyes
- No compensation needed with common FITC/GFP + PE combinations in flow cytometry
- No wash or RNase treatment needed.
SPECTRAL PROPERTIES
Excitation:
- 633 and 647 nm line optimal (Exmax 599 / 644 nm)
- 488, 514 and 568 nm lines, sub-optimal (only by flow cytometry)
Emission (instrument dependent):
- 665 nm to infra-red max 678 nm / 697 nm intercalated with dsDNA)
- Minimal overlap with vis range e.g. GFP and FITC
- Em. filters may include 695L, 715LP or 780 LP
Multi-wavelength imaging with UV / vis fluorochromes
- No fluorescence enhancement upon DNA binding
- Low photo-bleaching effect
- Compatible with optics of flow, laser scanning cytometers and confocal and lamp-based fluoroscence microscopes
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Flow Cytometry - DRAQ7™ (ab109202)
Jurkat cells exposed to 1μM staurosporine for 24 hours show DRAQ7™ staining (top half of the plot). These cells have compromised membranes that allow entry of DRAQ7™ in the cells.
Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical SchoolImmunohistochemistry (Frozen sections) - DRAQ7™ (ab109202)
Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)
Preparation:- Fix in 3%PFA in PBS for 30 min at RT
- Incubate in 7.5% sucrose-PBS for 3h at RT
- Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
- Embed the EBs in tissue-Tek OCT compound
- Cut frozen sections to 4-20 μm thickness
Primary antibody: Rabbit anti-laminin alpha 1, 1:400
Secondary antibody: Goat anti-rabbit IgG - H&L (AMCA) (ab123435)
Nuclei were counterstained stained with DRAQ7™ (ab109202)
Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical SchoolImmunohistochemistry (Frozen sections) - DRAQ7™ (ab109202)
Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)
Preparation:- Fix in 3%PFA in PBS for 30 min at RT
- Incubate in 7.5% sucrose-PBS for 3h at RT
- Incubate in 15% sucrose-PBS at 4°C overnight
- Embed the EBs in tissue-Tek OCT compound
- Cut frozen sections to 4-20 μm thickness
Primary antibody: Rabbit anti-laminin alpha 1, 1:400
Secondary antibody: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) (Goat Anti-Rabbit IgG H&L (FITC) ab97050), 1:200
F-actin was stained with CytoPainter F-actin staining kit (blue) (F-actin Staining Kit - Blue Fluorescence - Cytopainter ab112124), 1:1000
Nuclei were counterstained stained with DRAQ7™, 1:1000
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