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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Ready-to-use green stain for normalizing antibody signal intensity to total cell amounts during in-cell ELISA (ab111622).
In-Cell ELISA
Liquid
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application In-Cell ELISA | Reactivity Reacts | Dilution info - | Notes 50 µL/ well fixed cells |
Ready-to-use green stain for normalizing antibody signal intensity to total cell amounts during in-cell ELISA (ab111622).
In-Cell ELISA
Liquid
Constituents: Water, 0.81% Sodium chloride, 0.3% Janus Green B, 0.216% Dibasic monohydrogen sodium phosphate, 0.02% Potassium chloride, 0.02% Potassium phosphate monobasic
Blue Ice
+4°C
Ready-to-use green stain for normalizing antibody signal intensity to total cell amounts during in-cell ELISA (ab111622).
Cells fixed to a solid surface (typically in microplate wells) can be stained by adding 1X Janus Green Stain. Incubate cells for 5 minutes at room temperature. Dye should be removed and washed 5 times in ultrapure water or until excess dye is removed. The last wash should be removed and add 0.1 mL 0.5 M HCl per well and incubate for 10 minutes. Shake the plate for 10 seconds, and measure the OD 595 nm using a standard microplate spectrophotometer.
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