Ready-to-use green stain for normalizing antibody signal intensity to total cell amounts during in-cell ELISA (ab111622).
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application In-Cell ELISA | Reactivity Reacts | Dilution info - | Notes 50 µL/ well fixed cells |
Ready-to-use green stain for normalizing antibody signal intensity to total cell amounts during in-cell ELISA (ab111622).
Constituents: Water, 0.81% Sodium chloride, 0.3% Janus Green B, 0.216% Disodium hydrogenorthophosphate, 0.02% Potassium chloride, 0.02% Potassium phosphate monobasic
Ready-to-use green stain for normalizing antibody signal intensity to total cell amounts during in-cell ELISA (ab111622).
Cells fixed to a solid surface (typically in microplate wells) can be stained by adding 1X Janus Green Stain. Incubate cells for 5 minutes at room temperature. Dye should be removed and washed 5 times in ultrapure water or until excess dye is removed. The last wash should be removed and add 0.1 mL 0.5 M HCl per well and incubate for 10 minutes. Shake the plate for 10 seconds, and measure the OD 595 nm using a standard microplate spectrophotometer.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com