Skip to main content

Ready-to-use green stain for normalizing antibody signal intensity to total cell amounts during in-cell ELISA (ab111622).

Be the first to review this product! Submit a review

Key facts

Applications

In-Cell ELISA

Form

Liquid

Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

Reactivity data

Application

In-Cell ELISA

Reactivity

Reacts

Dilution info

-

Notes

50 µL/ well fixed cells

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Ready-to-use green stain for normalizing antibody signal intensity to total cell amounts during in-cell ELISA (ab111622).

Key facts

Applications

In-Cell ELISA

Form

Liquid

Storage buffer

Constituents: Water, 0.81% Sodium chloride, 0.3% Janus Green B, 0.216% Dibasic monohydrogen sodium phosphate, 0.02% Potassium chloride, 0.02% Potassium phosphate monobasic

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

Ready-to-use green stain for normalizing antibody signal intensity to total cell amounts during in-cell ELISA (ab111622).

Cells fixed to a solid surface (typically in microplate wells) can be stained by adding 1X Janus Green Stain. Incubate cells for 5 minutes at room temperature. Dye should be removed and washed 5 times in ultrapure water or until excess dye is removed. The last wash should be removed and add 0.1 mL 0.5 M HCl per well and incubate for 10 minutes. Shake the plate for 10 seconds, and measure the OD 595 nm using a standard microplate spectrophotometer.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

0 product images

    Downloads

    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

    For licensing inquiries, please contact partnerships@abcam.com

    There was a problem

    We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com