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AB138905

Nuclear Green DCS1

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(6 Publications)

Nuclear Green DCS1 is a fluorogenic, DNA-selective and cell impermeant green fluorescent dye for analyzing DNA content in dead cells.
2 Images
Functional Studies - Nuclear Green DCS1 (AB138905)
  • FuncS

Unknown

Functional Studies - Nuclear Green DCS1 (AB138905)

Excitation and emission spectra for ab138905 Nuclear Green DCS1 bound to DNA in PBS (pH 7.4).

Immunocytochemistry/ Immunofluorescence - Nuclear Green DCS1 (AB138905)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Nuclear Green DCS1 (AB138905)

ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed and then incubated in 1% BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 5μg/ml) overnight at +4°C. The secondary antibody (red) was ab150115 Alexa Fluor® 647 goat anti-mouse IgG (H+L) used at 2μg/ml for 1h. Nuclear Green DCS1 (ab138905) was used to stain the cell nuclei (green) at a 1/1500 dilution with a 60 minute incubation.

Key facts

Applications

ICC/IF, Flow Cyt

applications

Target

Nuclear Green DCS1

target

Form

Liquid

form

Storage buffer

Constituents: 99% Dimethylsulfoxide, 1% Nuclear Green

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Nuclear Green DCS1 is a fluorogenic, DNA-selective and cell impermeant green fluorescent dye for analyzing DNA content in dead cells. The Nuclear Green DCS1 has its fluorescence significantly enhanced upon binding to double-stranded DNA. It can be used in fluorescence imaging, microplate and flow cytometry applications. This DNA-binding dye might be used for multicolor analysis of dead cells.

SPECTRAL PROPERTIES:

Excitation: Exmax 503 nm

Emission: Emmax 526 nm

Provided at 5mM. Recommended use at 2 - 10 uM.

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Nuclear Green DCS1
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

STAR protocols 2:100263 PubMed33490979

2021

Protocol for determining zinc-dependent β cell-selective small-molecule delivery in mouse pancreas.

Applications

Unspecified application

Species

Unspecified reactive species

Timothy M Horton,Benjamin R Kraemer,Justin P Annes

Genome biology 21:279 PubMed33203452

2020

A versatile toolkit for CRISPR-Cas13-based RNA manipulation in Drosophila.

Applications

Unspecified application

Species

Unspecified reactive species

Nhan Huynh,Noah Depner,Raegan Larson,Kirst King-Jones

Nature communications 11:1185 PubMed32132543

2020

Therapeutic potential of KLF2-induced exosomal microRNAs in pulmonary hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Hebah A Sindi,Giusy Russomanno,Sandro Satta,Vahitha B Abdul-Salam,Kyeong Beom Jo,Basma Qazi-Chaudhry,Alexander J Ainscough,Robert Szulcek,Harm Jan Bogaard,Claire C Morgan,Soni S Pullamsetti,Mai M Alzaydi,Christopher J Rhodes,Roberto Piva,Christina A Eichstaedt,Ekkehard Grünig,Martin R Wilkins,Beata Wojciak-Stothard

Nature communications 10:4452 PubMed31575873

2019

The ubiquitin-like modifier FAT10 interferes with SUMO activation.

Applications

Unspecified application

Species

Unspecified reactive species

Annette Aichem,Carolin Sailer,Stella Ryu,Nicola Catone,Nicolas Stankovic-Valentin,Gunter Schmidtke,Frauke Melchior,Florian Stengel,Marcus Groettrup

Experimental animals 68:499-509 PubMed31189761

2019

Generation of bicistronic reporter knockin mice for visualizing germ layers.

Applications

Unspecified application

Species

Unspecified reactive species

Hayate Suzuki,Tra Thi Huong Dinh,Yoko Daitoku,Yoko Tanimoto,Kanako Kato,Takuya Azami,Masatsugu Ema,Kazuya Murata,Seiya Mizuno,Fumihiro Sugiyama

Scientific reports 7:9628 PubMed28852121

2017

Templated Assembly of Collagen Fibers Directs Cell Growth in 2D and 3D.

Applications

Unspecified application

Species

Unspecified reactive species

G Y Liu,R Agarwal,K R Ko,M Ruthven,H T Sarhan,J P Frampton
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

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Alternative Product
Reagents, Controls & Accessories

AB138904

Nuclear Green LCS1

dyes

nuclear-green-lcs1-ab138904

2

(1 reviews)

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