Nuclear Green DCS1 is a fluorogenic, DNA-selective and cell impermeant green fluorescent dye for analyzing DNA content in dead cells.
Images
Publications
Filter by
- STAR protocols 2:1002632021Protocol for determining zinc-dependent β cell-selective small-molecule delivery in mouse pancreas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTimothy M Horton et. al.PubMed 33490979
- Genome biology 21:2792020A versatile toolkit for CRISPR-Cas13-based RNA manipulation in Drosophila.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNhan Huynh et. al.PubMed 33203452
- Nature communications 11:11852020Therapeutic potential of KLF2-induced exosomal microRNAs in pulmonary hypertension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHebah A Sindi et. al.PubMed 32132543
- Nature communications 10:44522019The ubiquitin-like modifier FAT10 interferes with SUMO activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnnette Aichem et. al.PubMed 31575873
- Experimental animals 68:499-5092019Generation of bicistronic reporter knockin mice for visualizing germ layers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHayate Suzuki et. al.PubMed 31189761
- Scientific reports 7:96282017Templated Assembly of Collagen Fibers Directs Cell Growth in 2D and 3D.Applications:Unspecified applicationReactive species:Unspecified reactive speciesG Y Liu et. al.PubMed 28852121
Key facts
- Applications
- Flow Cyt, ICC/IF
- Target
- Nuclear Green DCS1
- Form
- Liquid
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Flow Cyt | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Recommended products
Nuclear Green DCS1 is a fluorogenic, DNA-selective and cell impermeant green fluorescent dye for analyzing DNA content in dead cells.
Key facts
- Applications
- Flow Cyt, ICC/IF
- Target
- Nuclear Green DCS1
- Form
- Liquid
- Storage buffer
Constituents: 99% Dimethylsulfoxide, 1% Nuclear Green
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Store in the dark
Notes
Nuclear Green DCS1 is a fluorogenic, DNA-selective and cell impermeant green fluorescent dye for analyzing DNA content in dead cells. The Nuclear Green DCS1 has its fluorescence significantly enhanced upon binding to double-stranded DNA. It can be used in fluorescence imaging, microplate and flow cytometry applications. This DNA-binding dye might be used for multicolor analysis of dead cells.
SPECTRAL PROPERTIES:
Excitation: Exmax 503 nm
Emission: Emmax 526 nm
Provided at 5mM. Recommended use at 2 - 10 uM.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunocytochemistry/ Immunofluorescence - Nuclear Green DCS1 (ab138905)
ICC/IF image of Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 stained HeLa cells. The cells were 100% methanol fixed and then incubated in 1% BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, 5μg/ml) overnight at +4°C. The secondary antibody (red) was Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) ab150115 Alexa Fluor® 647 goat anti-mouse IgG (H+L) used at 2μg/ml for 1h. Nuclear Green DCS1 (ab138905) was used to stain the cell nuclei (green) at a 1/1500 dilution with a 60 minute incubation.
Functional Studies - Nuclear Green DCS1 (ab138905)
Excitation and emission spectra for ab138905 Nuclear Green DCS1 bound to DNA in PBS (pH 7.4).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com