The Hoechst stains are a family of fluorescent stains for labeling DNA used in fluorescence microscopy and flow cytometry.
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- Cell death & disease 11:1382020Loss of RE-1 silencing transcription factor accelerates exocrine damage from pancreatic injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulie K Bray et. al.PubMed 32080178
- Cell death & disease 4:e9762013TRIM32-dependent transcription in adult neural progenitor cells regulates neuronal differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesA-L Hillje et. al.PubMed 24357807
Key facts
- Applications
- Flow Cyt, ICC/IF
- Target
- Nuclear yellow (Hoechst S769121)
- Form
- Lyophilized
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Flow Cyt | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info - | Notes - |
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The Hoechst stains are a family of fluorescent stains for labeling DNA used in fluorescence microscopy and flow cytometry.
Key facts
- Applications
- Flow Cyt, ICC/IF
- Target
- Nuclear yellow (Hoechst S769121)
- Form
- Lyophilized
- Storage buffer
Constituents: 100% Nuclear yellow
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Store in the dark
Notes
The Hoechst stains are a family of fluorescent stains for labeling DNA used in fluorescence microscopy and flow cytometry. Because these fluorescent stains label DNA, they are also commonly used to visualize nuclei and mitochondria.
The long-wavelength tracer Nuclear yellow is often combined with the popular retrograde tracer True Blue for two-color neuronal mapping. In neuronal cells, Nuclear yellow primarily stains the nucleus with yellow fluorescence, whereas True Blue is a UV light–excitable, divalent cationic dye that stains the cytoplasm with blue fluorescence. Both Nuclear yellow and True Blue are stable when subjected to immunohistochemical processing and can be used to photoconvert DAB into an insoluble, electron-dense reaction product.
Molecular Weight: 651.01
Appearance: Light yellow powder
Solvents: water or dimethylsulfoxide (DMSO)
SPECTRAL PROPERTIES:
Excitation: Exmax 355 nm
Emission: Emmax 495 nm*
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2 product images
Immunocytochemistry/ Immunofluorescence - Nuclear yellow (Hoechst S769121) (ab138903)
ICC/IF image of Anti-beta Tubulin antibody - Loading Control ab6046 stained HeLa cells. The cells were 100% methanol fixed and then incubated in 1% BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-beta Tubulin antibody - Loading Control ab6046, 1μg/ml) overnight at +4°C. The secondary antibody (orange) was Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150064 Alexa Fluor® 594 donkey anti-rabbit IgG (H+L) used at 2μg/ml for 1h. Nuclear Yellow (Hoechst S769121) (ab138903) was used to stain the cell nuclei (purple/blue) at a 250μg/ml dilution with a 60 minute incubation.
Chemical Structure - Nuclear yellow (Hoechst S769121) (ab138903)
Chemical structure of ab138903 Nuclear yellow (Hoechst S769121).
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