Nuclear yellow (Hoechst S769121)

The Hoechst stains are a family of fluorescent stains for labeling DNA used in fluorescence microscopy and flow cytometry. Because these fluorescent stains label DNA, they are also commonly used to visualize nuclei and mitochondria.


The long-wavelength tracer Nuclear yellow is often combined with the popular retrograde tracer True Blue for two-color neuronal mapping. In neuronal cells, Nuclear yellow primarily stains the nucleus with yellow fluorescence, whereas True Blue is a UV light–excitable, divalent cationic dye that stains the cytoplasm with blue fluorescence. Both Nuclear yellow and True Blue are stable when subjected to immunohistochemical processing and can be used to photoconvert DAB into an insoluble, electron-dense reaction product.



Molecular Weight: 651.01

Appearance: Light yellow powder

Solvents: water or dimethylsulfoxide (DMSO)

SPECTRAL PROPERTIES:

Excitation: Ex_max 355 nm

Emission: Em_max 495 nm*