Antibody pairs, consisting of carefully selected and matched capture and detection antibodies, are vital components in ELISA kits and other sandwich-based immunoassays. These pairs are designed to ensure high specificity and sensitivity for the target antigen, providing researchers with reliable tools for quantifying proteins. At Abcam, we have developed over 1800 antibody pairs using our recombinant rabbit monoclonal antibodies to ensure optimized specificity, sensitivity, and reproducibility.

Key features

• We use monoclonal antibodies for the best sensitivity: monoclonal antibodies bind to one specific epitope on the target antigen, reducing cross-reactivity and background noise, offering improved signal quality and sensitivity.

^{Fig. 1 A rabbit monoclonal antibody pair for Human IL-1Ra allows a significant gain in sensitivity compared to a rabbit polyclonal pair to the same target}

• Monoclonal ELISA antibody pairs provide better reproducibility: they're produced from a single clone, ensuring uniform affinity and performance across experiments, which enhances reproducibility.

^{Fig 2. Lot-to-lot comparison of polyclonal versus monoclonal antibody-based ELISA detection.
Left panel: Rabbit polyclonal detector shows significant variability between lots when measuring fibrinogen (ng/mL), with signal-to-noise ratios differing across concentrations.
Right panel: Rabbit monoclonal antibody pair demonstrates consistent performance between lots when detecting mouse IL-33 (pg/mL), as indicated by overlapping O.D. (450 nm) curves.}

Our pairs are provided as individual capture and detector antibodies, unconjugated and in a carrier-free format (free from BSA and Azide). This means they are ready for conjugation, as there will be no interference from proteins or stabilizers with the conjugation site, and you can select your label of choice.

Key benefits of carrier-free recombinant monoclonal antibody pairs

Streamlined workflow

Using pre-validated antibody pairs reduces the time and effort required to develop and optimize ELISA assays and other sandwich-based immunoassays.

Enhanced sensitivity and reproducibility

Consistent pair performance leads to reliable experimental outcomes.

Versatility

Suitable for a wide array of applications, from basic research to specific disease biomarker detection.

Using our recombinant monoclonal antibody pairs in your ELISA/immunoassays workflows not only simplifies the experimental setup but also enhances the reliability and accuracy of your results, empowering your research with precision.