Chemiluminescent ELISA kits use light-emitting reactions to offer highly sensitive and rapid detection of biomarkers.
These kits use a chemiluminescent substrate to produce light in response to antigen-antibody interactions, which allows for greater sensitivity and broader dynamic range than traditional ELISA.
Our 90-minute colorimetric SimpleStep® ELISA kits have saved researchers around the world 82 years at the bench. To raise the bar, we’ve developed a chemiluminescent SimpleStep® ELISA with an incubation time of just 30 minutes and an overall assay time of 55 minutes, so you can get insights 6x faster.
SimpleStep Ignite™ ELISA is powered by the same premium antibodies as our colorimetric version, but offers increased sensitivity and broader dynamic range.
How does SimpleStep Ignite work?
SimpleStep Ignite™ ELISA relies on the same streamlined principle as our sandwich colorimetric SimpleStep® ELISA kits, with the same single-wash standardized workflow.
Benefits of our SimpleStep Ignite ELISA kits
SimpleStep Ignite ELISA offers
What it means for you
Higher sensitivity: the bottom of our measuring range is over 50× lower than the top-cited ELISA kits
Reliably quantify low concentrations of your target of interest
Broader measuring range: up to 35x broader measuring range compared to our colorimetric SimpleStep® ELISA kits
Gain time and get more results out of your samples with fewer dilutions
High reproducibility and specificity from recombinant antibodies
Consistent and reliable results from the first attempt
Validation on biological samples
Guaranteed reaction with the endogenous protein
Single-wash, 55 minute standardized protocol
Get reliable results with reduced error risk in under an hour
Example of increased sensitivity and dynamic range for Human Pro-Collagen I alpha 1 SimpleStep Ignite® ELISA kit (orange bar) (ab325360) compared to the corresponding colorimetric SimpleStep® ELISA kit and the leading colorimetric competitor kit. (SSE: SimpleStep® ELISA)
Frequently asked questions
How do chemiluminescent ELISA kits differ from traditional colorimetric ELISA kits?
Chemiluminescent ELISA kits use a luminescent substrate that emits light as a signal, which is then measured via a luminometer. This method generally provides higher sensitivity and a broader dynamic range compared to the colorimetric method, which relies on a chromogenic substrate and optical density measurements.
Are these kits suitable for all types of samples?
Chemiluminescent ELISA kits can be used with a variety of sample types, including serum, plasma, cell culture supernatants, and other biological fluids. However, it's important to consider a specific kit's compatibility with your sample type for optimal results.
What equipment is needed to use a chemiluminescent ELISA kit?
You will need a plate reader equipped to detect chemiluminescence.
