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AB133054

11-dehydro-TXB2 ELISA Kit

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(1 Publication)

11-dehydro-TXB2 ELISA Kit is a competitive ELISA designed to quantify 11-dehydro-TXB2 with a sensitivity of 4.31 pg/mL

- Colorimetric competitive ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 9.8 - 10000 pg/ml

View Alternative Names

11-dehydro-Thromboxane B2

1 Images
Competitive ELISA - 11-dehydro-TXB2 ELISA Kit (AB133054)
  • cELISA

Supplier Data

Competitive ELISA - 11-dehydro-TXB2 ELISA Kit (AB133054)

Typcial Standard Curve.

Representative Standard Curve using ab133054.

Key facts

Detection method

Colorimetric

Sample types

Urine, Cell culture supernatant

Reacts with

Human

Assay type

Competitive

Sensitivity

= 4.31 pg/mL

Range

9.8 - 10000 pg/mL

Assay time

3h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's 11-dehydro-TXB2 in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 11-dehydro-TXB2 in tissue culture media and urine.

A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-11-dehydro-TXB2 antigen and a polyclonal rabbit antibody specific to 11-dehydro-TXB2. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of 11-dehydro-TXB2 captured in the plate.

11-dehydro-Thromboxane B2 (11-dehydro-TXB2) is a major metabolite of Thromboxane B2 (TXB2) found in urine and plasma. It is produced by the dehydrogenation of the alcohol group on the C11 position of TXB2 by the enzyme 11-OH-dehydrogenase. The detection of 11-dehydro-TXB2 is often used to measure thromboxane production in vivo. Monitoring levels of this metabolite is a helpful tool in the study of several diseases, such as liver cirrhosis, cystic fibrosis, mastocytosis, systemic lupus erythematosus, thrombosis diseases, and other diseases involving platelet activation.11-dehydro-TXB2 has also been used in diabetes and asthma studies.

Cross reactivities:

Compound Cross Reactivity
11-dehydro-TXB2 100%
PGE2 1.85%
TXB2 0.4%
PGF2α 0.2%
6-keto-PGF1α 0.17%
PGD2 0.1%
2,3-dinor TXB2 0.1%
8-iso-PGF2α 0.07%
11-b-PGF2α 0.05%
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Precision

[ { "reproducibilityType": "Inter", "sample": "High", "replicates": 8, "mean": "1250 pg/mL", "standardDeviation": null, "coefficientOfVariability": "16.3" }, { "reproducibilityType": "Inter", "sample": "Low conc", "replicates": 8, "mean": "72 pg/mL", "standardDeviation": null, "coefficientOfVariability": "17.5" }, { "reproducibilityType": "Inter", "sample": "Medium conc", "replicates": 8, "mean": "216 pg/mL", "standardDeviation": null, "coefficientOfVariability": "12.6" }, { "reproducibilityType": "Intra", "sample": "High", "replicates": 16, "mean": "318 pg/mL", "standardDeviation": null, "coefficientOfVariability": "8.1" }, { "reproducibilityType": "Intra", "sample": "Low conc", "replicates": 16, "mean": "67 pg/mL", "standardDeviation": null, "coefficientOfVariability": "13.2" }, { "reproducibilityType": "Intra", "sample": "Medium conc", "replicates": 16, "mean": "165 pg/mL", "standardDeviation": null, "coefficientOfVariability": "15.3" } ]
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Recovery

[ { "sample": "Urine", "range": null, "average": "= 112.2" }, { "sample": "Tissue Culture Media", "range": null, "average": "= 104.8" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

11-dehydro-TXB2 is a stable metabolite of thromboxane B2 (TXB2) formed by the action of thromboxane synthase. It is often used as a marker to measure thromboxane production in scientific studies. 11-dehydro-TXB2 has a molecular mass of approximately 368.5 g/mol. This compound is generally detected in urine where its levels reflect the overall thromboxane A2 production in the body. The conversion from TXB2 occurs rapidly making it a critical analyte in the study of thromboxane activity.
Biological function summary

11-dehydro-TXB2 helps assess platelet function and plays an integral role in understanding thrombotic processes. This metabolite is not part of a larger protein complex but it is an important player in systems where platelet aggregation and vasoconstriction occur. 11-dehydro-TXB2 is frequently used in research to investigate cardiovascular health providing insights into the regulation and disruption of normal platelet function.

Pathways

11-dehydro-TXB2 is a significant part of the arachidonic acid metabolism pathway. This pathway involves the conversion of arachidonic acid into various eicosanoids including thromboxane A2 which is quickly converted to TXB2 and subsequently to 11-dehydro-TXB2. Related proteins in this pathway include cyclooxygenase enzymes (COX-1 and COX-2) which facilitate the initial stages of eicosanoid synthesis.

11-dehydro-TXB2 is closely linked to cardiovascular diseases such as myocardial infarction and stroke. Elevated levels of 11-dehydro-TXB2 often indicate increased thromboxane production correlating with heightened thrombotic risk. In these disorders the metabolite serves as a biomarker for the effectiveness of antiplatelet therapy such as aspirin which targets and reduces thromboxane A2 synthesis through the inhibition of COX enzymes.
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Product protocols

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Target data

See full target information 11-dehydro-TXB2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biochimica et biophysica acta. Molecular basis of 1864:2901-2912 PubMed29864522

2018

Chemokine CC-motif ligand 2 participates in platelet function and arterial thrombosis by regulating PKCα-P38MAPK-HSP27 pathway.

Applications

ELISA

Species

Unspecified reactive species

Dan Liu,Yu Cao,Xiaolin Zhang,Chengfei Peng,Xiaoxiang Tian,Chenghui Yan,Yanxia Liu,Meili Liu,Yaling Han
View all publications
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websiteProtocolBooklet
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