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AB133034

12(S)-HETE ELISA Kit

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(8 Publications)

12(S)-HETE ELISA Kit is a Competitive ELISA for the measurement of 12(S)-HETE in in Biofluids, Cell Culture Media samples.
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Competitive ELISA - 12(S)-HETE ELISA Kit (AB133034)
  • cELISA

Supplier Data

Competitive ELISA - 12(S)-HETE ELISA Kit (AB133034)

Representative Standard Curve using ab133034

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant

Assay type

Competitive

Sensitivity

= 146.3 pg/mL

Range

195 - 50000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's 12(S)-HETE in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 12(S)-HETE in cell culture supernatants and plasma (heparin, EDTA).

A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-12(S)-HETE antigen and a polyclonal rabbit antibody specific to 12(S)-HETE. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the alkaline phosphatase enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of 12(S)-HETE captured in the plate.

12(S)-HETE is the stereo specific hydroxy product from the reduction of 12(S)-hydroperoxy tetraenoic eicosatetraenoic acid [12(S)-HpETE], which itself is a 12-lipoxygenase metabolite of arachidonic acid. 12(S)-HETE has been shown to be chemotactic and chemokinetic for polymorphonuclear leukocytes and vascular smooth cells. It also acts as a second messenger in angiotensin-II induced aldosterone production. Evidence also suggests that 12(S)-HETE is involved in suppressing renin production, stimulating insulin secretion by pancreatic tissue, inducing endothelial cell retraction and tumor cell adhesion.

Cross Reactivity

Compound % Cross Reactivity
12(S)-HETE 100
12(R)-HETE 2.5
15-HETE 0.3
5(S)-HETE 0.2
8,15-diHETE 0.1
5,15-diHETE 0.1
PGE2 0.1
PGF2α 0.1
PGD2 0.1
6-keto-PGF1α 0.1
Thromboxane B2 0.1
Arachidonic Acid 0.1
Leukotriene B4 0.1
Leukotriene C4 0.1
Leukotriene D4 0.1
Leukotriene E4 0.1
8-HETE <0.1
9-HETE <0.1
11-HETE <0.1
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Precision

[ { "reproducibilityType": "Inter", "sample": "Buffer", "replicates": 0, "mean": "5294 pg/mL", "standardDeviation": null, "coefficientOfVariability": "= 20.8" }, { "reproducibilityType": "Inter", "sample": "Buffer", "replicates": 0, "mean": "224 pg/mL", "standardDeviation": null, "coefficientOfVariability": "= 4.1" }, { "reproducibilityType": "Inter", "sample": "Buffer", "replicates": 0, "mean": "1127 pg/mL", "standardDeviation": null, "coefficientOfVariability": "= 9.1" }, { "reproducibilityType": "Intra", "sample": "Buffer", "replicates": 16, "mean": "4762 pg/mL", "standardDeviation": null, "coefficientOfVariability": "= 15.5" }, { "reproducibilityType": "Intra", "sample": "Buffer", "replicates": 16, "mean": "342 pg/mL", "standardDeviation": null, "coefficientOfVariability": "= 5.2" }, { "reproducibilityType": "Intra", "sample": "Buffer", "replicates": 16, "mean": "1153 pg/mL", "standardDeviation": null, "coefficientOfVariability": "= 10.1" } ]
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Recovery

[ { "sample": "Cell culture media", "range": null, "average": "= 94" }, { "sample": "EDTA Plasma", "range": null, "average": "= 97" }, { "sample": "Heparin Plasma", "range": null, "average": "= 104" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab133034-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Unit", "name":"Plate Sealer", "number":"AB133034-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Goat anti-rabbit IgG Microplate (12 x 8 wells)", "number":"AB133034-CMP09", "productcode":"" }, { "size":"1 x 27 mL", "name":"20X Wash Buffer Concentrate", "number":"AB133034-CMP08", "productcode":"" }, { "size":"1 x 5 mL", "name":"12(S)-HETE Antibody", "number":"AB133034-CMP05", "productcode":"" }, { "size":"1 x 5 mL", "name":"12(S)-HETE Conjugate", "number":"AB133034-CMP06", "productcode":"" }, { "size":"1 x 0.5 mL", "name":"12(S)-HETE Standard", "number":"AB133034-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"pNpp Substrate", "number":"AB133034-CMP03", "productcode":"" }, { "size":"1 x 27 mL", "name":"Assay Buffer", "number":"AB133034-CMP01", "productcode":"" }, { "size":"1 x 5 mL", "name":"Stop Solution", "number":"AB133034-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

12(S)-HETE also known as 12(S)-hydroxyeicosatetraenoic acid is a bioactive lipid with a molecular mass of approximately 320 g/mol. It derives from arachidonic acid through the action of the 12-lipoxygenase enzyme. Cells such as platelets leukocytes and many types of epithelial cells express 12(S)-HETE. It plays a role in signaling mechanisms that manage various cellular processes such as inflammation and vascular function. Its effective concentration and local action make it integral to specific tissue environments.
Biological function summary

12(S)-HETE modulates cell movement proliferation and survival. It often interacts with membrane receptors and influences intracellular signal transduction pathways. Although not typically part of a large protein complex it acts closely with cellular structures to modify calcium signaling and gene expression. It impacts processes that could lead to tissue remodeling or repair especially under conditions of inflammatory stress.

Pathways

The role of 12(S)-HETE becomes evident in arachidonate metabolism and MAPK signaling. The lipid mediator influences signaling networks that are critical for immune responses and cellular stress reactions. Within these pathways it interacts with proteins such as cyclooxygenases and leukotrienes. These interactions suggest its modulatory capacity in balancing anti-inflammatory and pro-inflammatory signals.

12(S)-HETE contributes significantly to pathologies like cardiovascular disease and cancer. It regulates endothelial function and vessel tone affecting conditions like atherosclerosis. Cancerous tissues often show altered levels of 12(S)-HETE indicating its role in tumor growth and metastasis. In these diseases it may engage with proteins like VEGF and MMPs to promote angiogenesis and tissue invasion. Understanding its involvement provides insights into potential therapeutic target opportunities.
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Product protocols

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Target data

See full target information 12(S)-HETE
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Lung cellular and molecular physiology 325:L104-L113 PubMed37253655

2023

Hematopoietic 12/15-lipoxygenase activity negatively contributes to fungal-associated allergic asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Mgayya Makullah,Diandra A Ellis,MaryJane Jones,Chad Steele

Cell death & disease 13:548 PubMed35697672

2022

Hsp90 induces Acsl4-dependent glioma ferroptosis via dephosphorylating Ser637 at Drp1.

Applications

Unspecified application

Species

Unspecified reactive species

Zong Miao,Wei Tian,Yangfan Ye,Wei Gu,Zhongyuan Bao,Lei Xu,Guangchi Sun,Chong Li,Yiming Tu,Honglu Chao,Sin Man Lam,Ning Liu,Jing Ji

Oncology letters 22:781 PubMed34594422

2021

Ferroptosis is involved in the anti-tumor effect of lycorine in renal cell carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Du,Hong-Chao Zhao,Heng-Cheng Zhu,Yao Jin,Lei Wang

Nature communications 12:4220 PubMed34244497

2021

Prokineticin-2 prevents neuronal cell deaths in a model of traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongyuan Bao,Yinlong Liu,Binglin Chen,Zong Miao,Yiming Tu,Chong Li,Honglu Chao,Yangfan Ye,Xiupeng Xu,Guangchi Sun,Pengzhan Zhao,Ning Liu,Yan Liu,Xiaoming Wang,Sin Man Lam,Valerian E Kagan,Hülya Bayır,Jing Ji

Neuro-oncology 23:2014-2027 PubMed33984142

2021

MEF2C silencing downregulates NF2 and E-cadherin and enhances Erastin-induced ferroptosis in meningioma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongyuan Bao,Lingyang Hua,Yangfan Ye,Daijun Wang,Chong Li,Qing Xie,Hiroaki Wakimoto,Ye Gong,Jing Ji

Oncology letters 20:122 PubMed32863935

2020

Dihydrotanshinone I inhibits human glioma cell proliferation via the activation of ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Shougang Tan,Xiaoqun Hou,Lin Mei

Oncology reports 43:147-158 PubMed31789401

2019

ACSL4 suppresses glioma cells proliferation via activating ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Cheng,Yan-Qin Fan,Bao-Hui Liu,Han Zhou,Jun-Min Wang,Qian-Xue Chen

Cell death and differentiation 26:2284-2299 PubMed30737476

2019

Ischemia-induced ACSL4 activation contributes to ferroptosis-mediated tissue injury in intestinal ischemia/reperfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Li,Dongcheng Feng,Zhanyu Wang,Yan Zhao,Ruimin Sun,Donghai Tian,Deshun Liu,Feng Zhang,Shili Ning,Jihong Yao,Xiaofeng Tian
View all publications
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