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AB133031

15-deoxy-delta12,14-PGJ2 ELISA Kit

4

(1 Review)

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(3 Publications)

15-deoxy-delta12,14-PGJ2 ELISA Kit is a competitive ELISA designed to quantify 15-deoxy-delta12,14-PGJ2 with a sensitivity of 36.8 pg/mL

- Colorimetric competitive ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 195 - 200000 pg/ml

View Alternative Names

15-d-PGJ2, Prostaglandin J2

1 Images
Competitive ELISA - 15-deoxy-delta12,14-PGJ2 ELISA Kit (AB133031)
  • cELISA

Supplier Data

Competitive ELISA - 15-deoxy-delta12,14-PGJ2 ELISA Kit (AB133031)

Representative Standard Curve using ab133031.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Plasma, Cell culture supernatant

Reacts with

Human, Pig

Assay type

Competitive

Sensitivity

= 36.8 pg/mL

Range

195 - 200000 pg/mL

Assay time

5h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's 15-deoxy-delta12,14-PGJ2 in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 15-deoxy-delta12,14-PGJ2 in tissue culture media, saliva, urine, and plasma from a wide range of species.

A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-15-deoxy-delta12,14-PGJ2 antigen and a polyclonal rabbit antibody specific to 15-deoxy-delta12,14-PGJ2. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of 15-deoxy-delta12,14-PGJ2 captured in the plate.

15-deoxy-Δ12,14-PGJ2 is one of the ultimate dehydration products of PGD2. PGD2 is formed from PGH2 which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthase. In the aqueous solution PGD2 forms PGJ2. Albumin or other serum proteins present in the sample will convert PGD2 to the isomeric compound, Δ12-PGJ2. In males about 152 ng of Δ12-PGJ2 is excreted in urine per day, and in women about half this amount is excreted. 15-deoxy-Δ12,14-PGJ2 has been shown as an inducer of adipogenesis and an activator of the γ isoform of the peroxisome proliferation activated receptor (PPARγ).

Cross Reactivity

Compound Cross Reactivity
15-deoxy-Δ12,14-PGJ2 100%
PGJ2 49.2%
Δ12-PGJ2 5.99%
PGD2 4.92%
Arachidonic Acid 0.03%
PGE2 <0.01%
PGF2α <0.01%
9α,11β-PGF2α <0.01%
Thromboxane B2 <0.01%
2-Arachidonoylglycerol <0.01%
Anandamide <0.01%
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Precision

[ { "reproducibilityType": "Inter", "sample": "High", "replicates": 8, "mean": "7219 pg/mL", "standardDeviation": null, "coefficientOfVariability": "14.5" }, { "reproducibilityType": "Inter", "sample": "Low", "replicates": 8, "mean": "1195 pg/mL", "standardDeviation": null, "coefficientOfVariability": "15.7" }, { "reproducibilityType": "Inter", "sample": "Media", "replicates": 8, "mean": "4406 pg/mL", "standardDeviation": null, "coefficientOfVariability": "13" }, { "reproducibilityType": "Intra", "sample": "High", "replicates": 16, "mean": "4394 pg/mL", "standardDeviation": null, "coefficientOfVariability": "7.4" }, { "reproducibilityType": "Intra", "sample": "Low", "replicates": 16, "mean": "1136 pg/mL", "standardDeviation": null, "coefficientOfVariability": "5.6" }, { "reproducibilityType": "Intra", "sample": "Media", "replicates": 16, "mean": "3062 pg/mL", "standardDeviation": null, "coefficientOfVariability": "5.7" } ]
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Recovery

[ { "sample": "Plasma", "range": null, "average": "= 105" }, { "sample": "Urine", "range": null, "average": "= 113.9" }, { "sample": "Saliva", "range": null, "average": "= 94.4" }, { "sample": "Tissue Culture Media", "range": null, "average": "= 97.4" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

15-deoxy-Δ1214-prostaglandin J2 (15-deoxy-Δ1214-PGJ2) acts as a potent endogenous ligand for peroxisome proliferator-activated receptor gamma (PPARγ) a nuclear receptor. It is a derivative of prostaglandin D2 and its molecular mass is approximately 316.4 g/mol. This compound typically arises in various tissues during inflammation or oxidative stress situations where it executes its regulatory effects.
Biological function summary

Through binding to PPARγ 15-deoxy-Δ1214-PGJ2 plays a role in modulating gene expression involved in lipid metabolism and adipogenesis. Its interaction with PPARγ influences the expression of anti-inflammatory cytokines and influences the inflammatory response making it significant in controlling inflammation. This compound does not form part of a larger protein complex but acts as an important signaling molecule in cellular processes.

Pathways

Several signaling pathways are affected by 15-deoxy-Δ1214-PGJ2 especially the nuclear receptor signaling pathway including the PPAR pathway. This pathway impacts key metabolic processes and regulates genes like adipocyte fatty-acid-binding protein (aP2). Additionally it links with the NF-kappa B signaling pathway through its interaction with proteins like IκBα affecting the transcription of genes involved in immune responses.

Increased levels of 15-deoxy-Δ1214-PGJ2 appear related to metabolic disorders such as type 2 diabetes and cardiovascular diseases. PPARγ to which 15-deoxy-Δ1214-PGJ2 binds holds relevance for insulin sensitivity and glucose homeostasis in diabetes. Additionally in cardiovascular diseases its effects on inflammation and lipid metabolism become significant linking with proteins such as leptin highlighting its role in disease mechanisms.
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Product protocols

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Target data

See full target information 15-deoxy-delta 12,14-PGJ2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Andrologia 52:e13537 PubMed32100333

2020

Cyclooxygenase 1 (COX1) as an indicator of sperm quality in humans.

Applications

Unspecified application

Species

Unspecified reactive species

Yongjie Liu,Fan Zhang,Liang Dai

Molecular pain 13:1744806917704138 PubMed28381109

2017

Prostanoid-dependent spontaneous pain and PAR-dependent mechanical allodynia following oral mucosal trauma: involvement of TRPV1, TRPA1 and TRPV4.

Applications

Unspecified application

Species

Unspecified reactive species

Misa Ito,Kentaro Ono,Suzuro Hitomi,Tomotaka Nodai,Teppei Sago,Kiichiro Yamaguchi,Nozomu Harano,Kaori Gunnjigake,Ryuji Hosokawa,Tatsuo Kawamoto,Kiyotoshi Inenaga

Biochemical and biophysical research communication 473:1328-1333 PubMed27095392

2016

The low molecular weight fraction of human serum albumin upregulates production of 15d-PGJ2 in Peripheral Blood Mononuclear Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Gregory W Thomas,Leonard T Rael,Melissa Hausburg,Elizabeth D Frederick,Charles W Mains,Denetta Slone,Matthew M Carrick,David Bar-Or
View all publications
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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