AB133035

15(S)-HETE ELISA Kit

Name: 15(S)-HETE ELISA Kit (ab133035) | Abcam
Brand: Abcam Limited
SKU: AB133035
Price: 760 USD
Availability: InStock

Be the first to review this product! Submit a review

(9 Publications)

15(S)-HETE ELISA Kit is a competitive ELISA designed to quantify 15(S)-HETE with a sensitivity of 69.21 pg/mL

- Colorimetric competitive ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 78.1 - 20000 pg/ml
- Cited in over 10 publications

View Alternative Names

15(S)-hydroperoxy tetraenoic eicosatetraenoic acid

1 Images

Competitive ELISA - 15(S)-HETE ELISA Kit (AB133035)

cELISA

Supplier Data

Competitive ELISA - 15(S)-HETE ELISA Kit (AB133035)

Representative Standard Curve using ab133035

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Cell culture supernatant, Serum

Assay type

Competitive

Sensitivity

= 69.21 pg/mL

Range

78.1 - 20000 pg/mL

Assay Platform

Microplate

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Abcam's 15(S)-HETE in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 15-hydroxyeicosatetraenoic acid in plasma, serum, urine, tissue culture media and other biological fluids.

A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-15(S)-HETE antigen and a polyclonal rabbit antibody specific to 15(S)-HETE. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of 15(S)-HETE captured in the plate.

15(S)-HETE (15-hydroxyeicosatetraenoic acid) is the major hydroxy derivative of arachidonic acid when acted upon by 15-lipoxygenase (15-LOX). It is also the primary monohydroxy acid synthesized by the lipoxygenase activity of Cyclooxygenase-1. Aspirin-mediated acetylation of the COX-1 enzyme results in 15(R)-HETE. Blood platelets, peripheral leukocytes, vascular smooth muscle and other cell types produce Type-1 15-LOX while prostate, lung, skin and cornea tissues produce Type-2. 15-HETE has been proposed to act as a paracrine regulator of smooth muscle and lung neutrophil recruitment due in part to its incorporation into tracheal epithelium at the sn-2 position of phosphatidylinositol. The phosphoinositol modification in turn is thought to affect signal transduction and the regulation of intracellular calcium. Interleukin-4 has been shown to regulate 15(S)-HETE expression and incorporation into cellular phospholipids. 15(S)-HETE binds to actin and the alpha-subunit of mitochondrial ATP synthase suggesting a more direct method in regulating some physiological activities. Increased levels of 15(S)-HETE are associated with asthma, rhinitis, chronic paranasal sinusitis and rheumatoid arthritis.

Cross Reactivity

Compound	% Cross Reactivity
15(S)-HETE	100
5,15-diHETE	1
8,15-diHETE	1
13(S)-HODE	0.6
5-HETE	0.1
PGB₂	0.1
PGD₂	0.1
PGF_2α	0.1
12(S)-HETE	<0.05
12(R)-HETE	<0.05
Arachidonic Acid	<0.05
PGE₂	<0.05
Linoleic Acid	<0.05

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "High", "replicates": 8, "mean": "4335 pg/mL", "standardDeviation": null, "coefficientOfVariability": "= 6.1" }, { "reproducibilityType": "Intra", "sample": "Medium conc", "replicates": 16, "mean": "853 pg/mL", "standardDeviation": null, "coefficientOfVariability": "= 9.8" }, { "reproducibilityType": "Inter", "sample": "Low conc", "replicates": 8, "mean": "192 pg/mL", "standardDeviation": null, "coefficientOfVariability": "= 19.1" }, { "reproducibilityType": "Intra", "sample": "Low conc", "replicates": 16, "mean": "181 pg/mL", "standardDeviation": null, "coefficientOfVariability": "= 15.8" }, { "reproducibilityType": "Intra", "sample": "High", "replicates": 16, "mean": "3929 pg/mL", "standardDeviation": null, "coefficientOfVariability": "= 3.4" }, { "reproducibilityType": "Inter", "sample": "Medium conc", "replicates": 8, "mean": "730 pg/mL", "standardDeviation": null, "coefficientOfVariability": "= 8.9" } ]

style

left-column

Recovery

[ { "sample": "EDTA Plasma", "range": null, "average": "= 105.4" }, { "sample": "Serum", "range": null, "average": "= 95.9" }, { "sample": "Tissue Culture Media", "range": null, "average": "= 100.3" }, { "sample": "Urine", "range": null, "average": "= 89.6" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab133035-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Unit", "name":"Plate Sealer", "number":"AB133035-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Goat anti-rabbit IgG Microplate (12 x 8 wells)", "number":"AB133035-CMP09", "productcode":"" }, { "size":"1 x 27 mL", "name":"20X Wash Buffer Concentrate", "number":"AB133035-CMP08", "productcode":"" }, { "size":"1 x 27 mL", "name":"Assay Buffer", "number":"AB133035-CMP01", "productcode":"" }, { "size":"1 x 500 µL", "name":"15(S)-HETE Standard", "number":"AB133035-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"pNpp Substrate", "number":"AB133035-CMP03", "productcode":"" }, { "size":"1 x 5 mL", "name":"15(S)-HETE Alkaline Phosphatase Conjugate", "number":"AB133035-CMP06", "productcode":"" }, { "size":"1 x 5 mL", "name":"15(S)-HETE Antibody", "number":"AB133035-CMP05", "productcode":"" }, { "size":"1 x 5 mL", "name":"Stop Solution", "number":"AB133035-CMP04", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

15(S)-HETE also known as 15(S)-hydroxyeicosatetraenoic acid is a bioactive lipid molecule with a molecular mass of approximately 320.5 g/mol. This lipid derives from arachidonic acid through the enzymatic action of lipoxygenases primarily 15-lipoxygenase (15-LOX). It is expressed in various tissues including the lung skin and inflammatory cells. Its production occurs predominantly in response to cellular conditions that activate lipoxygenase pathways contributing to its role in various bodily processes.

Biological function summary

15(S)-HETE functions as a signaling molecule involved in inflammation and cell growth regulation. It participates in modulating cellular responses including acting on endothelial cells where it influences vascular tone and permeability. 15(S)-HETE is not part of a larger protein complex but it interacts with receptors on the cell surface and intracellular signaling pathways. Its role in inflammatory responses and vascular homeostasis is critical for normal physiological processes.

Pathways

15(S)-HETE contributes significantly to the inflammation and arachidonic acid metabolism pathways. It is closely related to prostaglandin and leukotriene synthesis positioning it as an important player in the regulation of inflammation. Within these pathways it interacts with proteins such as cyclooxygenase enzymes and other lipoxygenase isozymes influencing their activities and the production of other eicosanoids that mediate inflammatory responses.

15(S)-HETE shows connections to asthma and atherosclerosis. In asthma it participates in the regulation of airway inflammation and bronchial reactivity often involving interactions with proteins such as cytokines and eosinophils that exacerbate the inflammatory response. For atherosclerosis it is implicated in plaque formation through its effects on endothelial cells and macrophages highlighting its role in modulating the chronic inflammatory environment associated with the disease.

style

left-column

Product protocols

style

left-column

Target data

See full target information 15(S)-HETE

style

left-column

Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 13:548 PubMed35697672

2022

Hsp90 induces Acsl4-dependent glioma ferroptosis via dephosphorylating Ser637 at Drp1.

Applications

Unspecified application

Species

Unspecified reactive species

Zong Miao,Wei Tian,Yangfan Ye,Wei Gu,Zhongyuan Bao,Lei Xu,Guangchi Sun,Chong Li,Yiming Tu,Honglu Chao,Sin Man Lam,Ning Liu,Jing Ji

Oncology letters 22:781 PubMed34594422

2021

Ferroptosis is involved in the anti-tumor effect of lycorine in renal cell carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Du,Hong-Chao Zhao,Heng-Cheng Zhu,Yao Jin,Lei Wang

Nature communications 12:4220 PubMed34244497

2021

Prokineticin-2 prevents neuronal cell deaths in a model of traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongyuan Bao,Yinlong Liu,Binglin Chen,Zong Miao,Yiming Tu,Chong Li,Honglu Chao,Yangfan Ye,Xiupeng Xu,Guangchi Sun,Pengzhan Zhao,Ning Liu,Yan Liu,Xiaoming Wang,Sin Man Lam,Valerian E Kagan,Hülya Bayır,Jing Ji

Neuro-oncology 23:2014-2027 PubMed33984142

2021

MEF2C silencing downregulates NF2 and E-cadherin and enhances Erastin-induced ferroptosis in meningioma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongyuan Bao,Lingyang Hua,Yangfan Ye,Daijun Wang,Chong Li,Qing Xie,Hiroaki Wakimoto,Ye Gong,Jing Ji

Oncology letters 20:122 PubMed32863935

2020

Dihydrotanshinone I inhibits human glioma cell proliferation via the activation of ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Shougang Tan,Xiaoqun Hou,Lin Mei

Oncology reports 43:147-158 PubMed31789401

2019

ACSL4 suppresses glioma cells proliferation via activating ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Cheng,Yan-Qin Fan,Bao-Hui Liu,Han Zhou,Jun-Min Wang,Qian-Xue Chen

Cell death and differentiation 26:2284-2299 PubMed30737476

2019

Ischemia-induced ACSL4 activation contributes to ferroptosis-mediated tissue injury in intestinal ischemia/reperfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Li,Dongcheng Feng,Zhanyu Wang,Yan Zhao,Ruimin Sun,Donghai Tian,Deshun Liu,Feng Zhang,Shili Ning,Jihong Yao,Xiaofeng Tian

PloS one 13:e0202693 PubMed30138423

2018

Hypoxic cardiac fibroblasts from failing human hearts decrease cardiomyocyte beating frequency in an ALOX15 dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Mikael Sandstedt,Victoria Rotter Sopasakis,Annika Lundqvist,Kristina Vukusic,Anders Oldfors,Göran Dellgren,Joakim Sandstedt,Lillemor Mattsson Hultén

PloS one 9:e88546 PubMed24533104

2014

Arachidonate 15-lipoxygenase enzyme products increase platelet aggregation and thrombin generation.

Applications

Unspecified application

Species

Unspecified reactive species

Carolina Vijil,Cecilia Hermansson,Anders Jeppsson,Göran Bergström,Lillemor Mattsson Hultén

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

15(S)-HETE ELISA Kit

Competitive ELISA - 15(S)-HETE ELISA Kit (AB133035)

Key facts

Detection method

Sample types

Assay type

Sensitivity

Range

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Complementary Products

Assay Kits

Secondary Antibodies

Assay Kits

Primary Antibodies

Product promise