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AB108667

17 beta Estradiol ELISA Kit

4

(2 Reviews)

|

(67 Publications)

The 17 beta Estradiol ELISA kit is a competitive ELISA designed to quantify 17-beta-estradiol (E2) with a sensitivity of 8.68 pg/mL.

- High sensitivity – detects 17 beta estradiol (E2) as low as 8.68 pg/mL
- Wide dynamic range – quantifies 20–2,000 pg/mL
- Compatible with multiple sample types – works with serum, plasma (citrate, EDTA, heparin), and urine
- Cited in over 55 publications

2 Images
Competitive ELISA - 17 beta Estradiol ELISA Kit (AB108667)
  • cELISA

Supplier Data

Competitive ELISA - 17 beta Estradiol ELISA Kit (AB108667)

Representative Standard Curve using ab108667

Competitive ELISA - 17 beta Estradiol ELISA Kit (AB108667)
  • cELISA

Lab

Competitive ELISA - 17 beta Estradiol ELISA Kit (AB108667)

17 beta Estradiol measured in biological fluids showing quantity (pg) per mL of tested sample. Samples were diluted 1-2 fold.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, EDTA Plasma, Heparin Plasma, Serum, Urine

Reacts with

Mammals

Assay type

Competitive

Sensitivity

= 8.68 pg/mL

Range

20 - 2000 pg/mL

Assay Platform

Microplate

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Secondary Antibodies

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Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Mouse and rat samples may need additional optimization.</p>" } } }
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Product details

17 beta Estradiol ELISA Kit ab108667 is a competitive ELISA kit to measure quantify 17-beta-estradiol (E2) with a sensitivity of 8.68 pg/mL.

How the assay works

17 beta Estradiol ELISA Kit ab108667 is a competitive ELISA. There are 2 types of competitive ELISAs:

  • antigen down: The sample antigen competes with a reference antigen for binding to a specific amount of labeled antibody. The reference antigen is pre-coated on a multi-well plate. The sample is added to the plate wells with a labeled antibody. Depending on the amount of antigen in the sample, more or fewer free antibodies will be available to bind the reference antigen. This means that the more of the antigen there is in the sample, the less reference antigen will be detected and the weaker the signal will be.
  • antibody down: the labeled antigen and the sample antigen (unlabeled) compete for binding to the primary antibody, which is immobilized on the plate. The lower the amount of antigen in the sample, the stronger the signal due to more labelled antigen in the well.

Assay specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility.

17 beta Estradiol ELISA Kit ab108667 protocol summary

  1. 1. A 96-well plate is provided in the kit, that has been precoated with anti-Estradiol IgG.
    2. Samples and the Estradiol-HRP conjugate are added to the wells, where Estradiol in the sample competes with the added Estradiol-HRP for antibody binding.
    3. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration.
    4. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow.
    5. The intensity of signal is inversely proportional to the amount of Estradiol in the sample and the intensity is measured at 450 nm.
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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 20, "mean": null, "standardDeviation": null, "coefficientOfVariability": "<= 9" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "<= 10" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108667-1x96Tests", "assetComponentDetails": [ { "size":"1 x 22 mL", "name":"17 beta Estradiol-HRP Conjugate", "number":"AB108667-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB108667-CMP07", "productcode":"" }, { "size":"1 x 500 µL", "name":"17 beta Estradiol Standard 2 (120 pg/mL)", "number":"AB108667-CMP10", "productcode":"" }, { "size":"1 x 500 µL", "name":"17 beta Estradiol Standard 1 (20 pg/mL)", "number":"AB108667-CMP09", "productcode":"" }, { "size":"1 x 500 µL", "name":"17 beta Estradiol Standard 4 (600 pg/mL)", "number":"AB108667-CMP12", "productcode":"" }, { "size":"1 x 500 µL", "name":"17 beta Estradiol Control", "number":"AB108667-CMP06", "productcode":"" }, { "size":"1 x 500 µL", "name":"17 beta Estradiol Standard 0 (0 pg/mL)", "number":"AB108667-CMP05", "productcode":"" }, { "size":"1 x 500 µL", "name":"17 beta Estradiol Standard 5 (2,000 pg/mL)", "number":"AB108667-CMP13", "productcode":"" }, { "size":"1 x 500 µL", "name":"17 beta Estradiol Standard 3 (300 pg/mL)", "number":"AB108667-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"10X Washing Solution", "number":"AB108667-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB108667-CMP14", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover foils", "number":"AB108667-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB108667-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-17 beta Estradiol IgG Coated Microplate (12 x 8 wells)", "number":"AB108667-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Estradiol 17-beta also known as 17-beta estradiol or beta estradiol is a steroid hormone with a molecular weight of approximately 272.38 g/mol. This hormone is the most biologically active form of estrogen and primarily synthesized by the ovaries though its presence is also noted in adipose tissue and the adrenal glands. Within the body 17-beta estradiol travels through the bloodstream to various tissues where it binds to estrogen receptors modulating gene expression and affecting numerous physiological processes.
Biological function summary

Estrogenic activities of estradiol 17-beta are important for reproductive and non-reproductive systems. It significantly influences the development and maintenance of female secondary sexual characteristics. This hormone also engages in the regulation of the menstrual cycle and reproductive function by interacting with other hormonal signals in the body. It can form complexes with estrogen receptors leading to DNA interactions that regulate the transcription of certain genes. Beyond reproduction estradiol 17-beta exerts effects on bone density cardiovascular health skin health and brain function.

Pathways

Various cellular systems utilize estradiol 17-beta in their signaling. It takes an essential role in the estrogen signaling pathway and interacts with the estrogen receptor pathway. These interactions commonly involve crosstalk with other proteins such as progesterone and androgens impacting cellular functions. The estrogen signaling pathway influence on gene transcription highlights its part in regulating cell proliferation apoptosis and differentiation processes.

Hormone imbalances involving estradiol 17-beta can lead to various conditions. It is notably associated with breast cancer where abnormal levels and receptor engagement can stimulate tumor growth. Osteoporosis is another disorder linked to estrogen levels as estradiol 17-beta preserves bone density by modulating bone resorption. Additionally hormonal fluctuations involving related proteins like aromatase and sex hormone-binding globulin (SHBG) can exacerbate the progression of these diseases further linking estradiol 17-beta to critical health outcomes.
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Product protocols

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Target data

See full target information Estradiol 17-Beta
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Publications (67)

Recent publications for all applications. Explore the full list and refine your search

Pharmacological research 215:107736 PubMed40228761

2025

Sex-specific effects of exogenous asparagine on colorectal tumor growth, 17β-estradiol levels, and aromatase.

Applications

Unspecified application

Species

Unspecified reactive species

Oladimeji Aladelokun,Katherine Benitez,Yuying Wang,Abhishek Jain,Domenica Berardi,Georgio Maroun,Xinyi Shen,Jatin Roper,Joanna Gibson,Kaelyn Sumigray,Sajid A Khan,Caroline H Johnson

Cardiovascular diabetology 24:39 PubMed39856754

2025

Obesity-induced mesenteric PVAT remodelling is sexually dimorphic, but not driven by ovarian hormones : Short title: Obesity induces sex-specific responses in mesenteric PVAT.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa Ivatt,Mhairi Paul,Allende Miguelez-Crespo,Patrick W F Hadoke,Matthew A Bailey,Ruth A Morgan,Mark Nixon

Journal of molecular neuroscience : MN 74:105 PubMed39496982

2024

Sex Differences in Blood Accumulation of Neurodegenerative-Related Proteins and Antioxidant Responses to Regular Physical Exercise.

Applications

Unspecified application

Species

Unspecified reactive species

Elisa Chelucci,Giorgia Scarfò,Rebecca Piccarducci,Antonio Rizza,Jonathan Fusi,Francesco Epifani,Sara Carpi,Beatrice Polini,Laura Betti,Barbara Costa,Sabrina Taliani,Vito Cela,Paolo Artini,Simona Daniele,Claudia Martini,Ferdinando Franzoni

Frontiers in molecular neuroscience 17:1459858 PubMed39498265

2024

Follicle-stimulating hormone induces depression-like phenotype by affecting synaptic function.

Applications

Unspecified application

Species

Unspecified reactive species

Liqin Huang,Shangqi Sun,Gege Jiang,Guanfeng Xie,Yunying Yang,Sichun Chen,Jiaying Luo,Chen Lv,Xiang Li,Jianming Liao,Zhihao Wang,Zhaohui Zhang,Jing Xiong

iScience 27:110585 PubMed39228787

2024

Reiterated male-to-female violence disrupts hippocampal estrogen receptor β expression, prompting anxiety-like behavior.

Applications

Unspecified application

Species

Unspecified reactive species

Jacopo Agrimi,Lucia Bernardele,Naeem Sbaiti,Marco Brondi,Donato D'Angelo,Marta Canato,Ivan Marchionni,Christian U Oeing,Giussy Barbara,Beatrice Vignoli,Marco Canossa,Nina Kaludercic,Gaya Spolverato,Anna Raffaello,Claudia Lodovichi,Marco Dal Maschio,Nazareno Paolocci

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2402393 PubMed39229959

2024

Sleep Deprivation Triggers the Excessive Activation of Ovarian Primordial Follicles via β2 Adrenergic Receptor Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lichun Weng,Hanqing Hong,Qinyu Zhang,Chengqi Xiao,Qiuwan Zhang,Qian Wang,Ju Huang,Dongmei Lai

Nutrients 16: PubMed39203867

2024

Sex- and Age-Specific Differences in Mice Fed a Ketogenic Diet.

Applications

Unspecified application

Species

Unspecified reactive species

Kenyon W Sprankle,Mya A Knappenberger,Erica J Locke,Jack H Thompson,Madison F Vinovrski,Kaylin Knapsack,Stephen C Kolwicz

Pathophysiology : the official journal of the International Society for Pathophysiology 31:408-419 PubMed39189167

2024

Ink Mitigates Dehydroepiandrosterone-Induced Insulin Resistance in Mouse Model of Polycystic Ovarian Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Prathyusha Yamarthi,Rama Satyasri Kotipalli,Samatasai Patnaik,Kv Veena,Muralidharan Kathirvel,Rajkumar Vutukuri,Manjula Bhanoori

The Journal of pharmacology and experimental therapeutics 391:258-271 PubMed38936979

2024

Chronic Administration of Cannabinoid Agonists ACEA, AM1241, and CP55,940 Induce Sex-Specific Differences in Tolerance and Sex Hormone Changes in a Chemotherapy-Induced Peripheral Neuropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Robert C Barnes,Henry Blanton,Canice Lei Dancel,Isabel Castro-Piedras,Boyd R Rorabaugh,Daniel J Morgan,Josée Guindon

JCI insight 9: PubMed38885308

2024

Myocardial infarction causes sex-dependent dysfunction in vagal sensory glutamatergic neurotransmission that is mitigated by 17β-estradiol.

Applications

Unspecified application

Species

Unspecified reactive species

Asokan Devarajan,Kerry Wang,Zulfiqar A Lokhandwala,Maryam Emamimeybodi,Kassandra Shannon,John D Tompkins,Andrea L Hevener,Aldons J Lusis,E Dale Abel,Marmar Vaseghi
View all publications
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