The 17 beta Estradiol ELISA kit is a competitive ELISA designed to quantify 17-beta-estradiol (E2) with a sensitivity of 8.68 pg/mL.
- High sensitivity – detects 17 beta estradiol (E2) as low as 8.68 pg/mL
- Wide dynamic range – quantifies 20–2,000 pg/mL
- Compatible with multiple sample types – works with serum, plasma (citrate, EDTA, heparin), and urine
- Cited in over 55 publications
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- Physiological reports 11:2023The effects of treadmill exercise on brain angiogenesis in ovariectomized rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEun-Jung Yoon,Jiwon Jeong,Eunji Yoon,Dongsun ParkPubMed 37962017
- Cell & bioscience 13:1652023Sex differences in paternal arsenic-induced intergenerational metabolic effects are mediated by estrogen.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanfeng Xue,Yingyun Gong,Xin Li,Fei Peng,Guolian Ding,Zhao Zhang,Junchao Shi,Ilma Saleh Savul,Yong Xu,Qi Chen,Leng Han,Shengyong Mao,Zheng SunPubMed 37691128
- iScience 26:1074702023Inhalation of ACE2 as a therapeutic target on sex-bias differences in SARS-CoV-2 infection and variant of concern.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Onodera,Jady Liang,Yuchong Li,Bryan Griffin,Thenuka Thanabalasingam,Cong Lu,JiaYi Zhu,Mingyao Liu,Theo Moraes,Wenhua Zheng,Jasmin Khateeb,Julie Khang,Yongbo Huang,Mirjana Jerkic,Masaki Nakane,Andrew Baker,Beverley Orser,Ya-Wen Chen,Gerald Wirnsberger,Josef M Penninger,Ori D Rotstein,Arthur S Slutsky,Yimin Li,Samira Mubareka,Haibo ZhangPubMed 37609639
- Metabolites 13:2023Trimethylamine N-Oxide Concentration and Blood Pressure in Young Healthy Men and Women: A Replicated Crossover Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamantha N Rowland,Liam M Heaney,Mariasole Da Boit,Stephen J BaileyPubMed 37512583
- Cells 12:2023Deficiency of Adipose Aryl Hydrocarbon Receptor Protects against Diet-Induced Metabolic Dysfunction through Sexually Dimorphic Mechanisms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNazmul Haque,Emmanuel S Ojo,Stacey L Krager,Shelley A TischkauPubMed 37443781
- Zygote (Cambridge, England) 31:386-3922023Sex steroid receptors in the ovarian follicles of the lizard .Applications:Unspecified applicationReactive species:Unspecified reactive speciesNorma Berenice Cruz-Cano,Uriel Ángel Sánchez-Rivera,Carmen Álvarez-Rodríguez,Mario Cárdenas-León,Martín Martínez-TorresPubMed 37212054
- International journal of molecular sciences 24:2023Sex Difference in Cardioprotection against Acute Myocardial Infarction in MAO-B Knockout Mice In Vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJacqueline Heger,Tamara Szabados,Paulin Brosinsky,Péter Bencsik,Péter Ferdinandy,Rainer SchulzPubMed 37047416
- Cells 12:2023Estrogen Mediates the Sexual Dimorphism of GT1b-Induced Central Pain Sensitization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJaesung Lee,Seohyun Chung,Minkyu Hwang,Yeongkag Kwon,Seung Hyun Han,Sung Joong LeePubMed 36899944
- Mayo Clinic proceedings 98:559-5682023Estradiol and Dihydrotestosterone Levels in COVID-19 Patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTaleen A MacArthur,Julie Goswami,Dhanya Ramachandran,Tammy L Price-Troska,Kaitlin A Lundell,Beth A Ballinger,Erica A Loomis,Stephanie F Heller,Daniel Stephens,Ryan T Hurt,Bradley R Salonen,Ravindra Ganesh,Grant M Spears,Kent R Bailey,Irshad H Chaudry,Myung S ParkPubMed 36872195
- Nature communications 14:182023Age-related matrix stiffening epigenetically regulates α-Klotho expression and compromises chondrocyte integrity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHirotaka Iijima,Gabrielle Gilmer,Kai Wang,Allison C Bean,Yuchen He,Hang Lin,Wan-Yee Tang,Daniel Lamont,Chia Tai,Akira Ito,Jeffrey J Jones,Christopher Evans,Fabrisia AmbrosioPubMed 36627269
Key facts
- Detection method
- Colorimetric
- Sample types
- Citrate plasma, EDTA Plasma, Heparin Plasma, Serum, Urine
- Assay type
- Competitive
- Reactive species
- Mammals
- Range
- 20 - 2000 pg/mL
- Sensitivity
- = 8.68 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application cELISA | Reactivity Reacts | Dilution info - | Notes Mouse and rat samples may need additional optimization. |
What's included?
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The 17 beta Estradiol ELISA kit is a competitive ELISA designed to quantify 17-beta-estradiol (E2) with a sensitivity of 8.68 pg/mL.
- High sensitivity – detects 17 beta estradiol (E2) as low as 8.68 pg/mL
- Wide dynamic range – quantifies 20–2,000 pg/mL
- Compatible with multiple sample types – works with serum, plasma (citrate, EDTA, heparin), and urine
- Cited in over 55 publications
Key facts
- Detection method
- Colorimetric
- Sample types
- Citrate plasma, EDTA Plasma, Heparin Plasma, Serum, Urine
- Assay type
- Competitive
- Reactive species
- Mammals
- Range
- 20 - 2000 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- = 8.68 pg/mL
- General recovery
- 101.09%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 20 | mean - | SD - | C.V. <= 9 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 3 | mean - | SD - | C.V. <= 10 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
17 beta Estradiol ELISA Kit ab108667 is a competitive ELISA kit to measure quantify 17-beta-estradiol (E2) with a sensitivity of 8.68 pg/mL.
How the assay works
17 beta Estradiol ELISA Kit ab108667 is a competitive ELISA. There are 2 types of competitive ELISAs:
- antigen down: The sample antigen competes with a reference antigen for binding to a specific amount of labeled antibody. The reference antigen is pre-coated on a multi-well plate. The sample is added to the plate wells with a labeled antibody. Depending on the amount of antigen in the sample, more or fewer free antibodies will be available to bind the reference antigen. This means that the more of the antigen there is in the sample, the less reference antigen will be detected and the weaker the signal will be.
- antibody down: the labeled antigen and the sample antigen (unlabeled) compete for binding to the primary antibody, which is immobilized on the plate. The lower the amount of antigen in the sample, the stronger the signal due to more labelled antigen in the well.
Assay specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility.
17 beta Estradiol ELISA Kit ab108667 protocol summary
- 1. A 96-well plate is provided in the kit, that has been precoated with anti-Estradiol IgG.
2. Samples and the Estradiol-HRP conjugate are added to the wells, where Estradiol in the sample competes with the added Estradiol-HRP for antibody binding.
3. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration.
4. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow.
5. The intensity of signal is inversely proportional to the amount of Estradiol in the sample and the intensity is measured at 450 nm.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Estradiol 17-beta also known as 17-beta estradiol or beta estradiol is a steroid hormone with a molecular weight of approximately 272.38 g/mol. This hormone is the most biologically active form of estrogen and primarily synthesized by the ovaries though its presence is also noted in adipose tissue and the adrenal glands. Within the body 17-beta estradiol travels through the bloodstream to various tissues where it binds to estrogen receptors modulating gene expression and affecting numerous physiological processes.
- Biological function summary
Estrogenic activities of estradiol 17-beta are important for reproductive and non-reproductive systems. It significantly influences the development and maintenance of female secondary sexual characteristics. This hormone also engages in the regulation of the menstrual cycle and reproductive function by interacting with other hormonal signals in the body. It can form complexes with estrogen receptors leading to DNA interactions that regulate the transcription of certain genes. Beyond reproduction estradiol 17-beta exerts effects on bone density cardiovascular health skin health and brain function.
- Pathways
Various cellular systems utilize estradiol 17-beta in their signaling. It takes an essential role in the estrogen signaling pathway and interacts with the estrogen receptor pathway. These interactions commonly involve crosstalk with other proteins such as progesterone and androgens impacting cellular functions. The estrogen signaling pathway influence on gene transcription highlights its part in regulating cell proliferation apoptosis and differentiation processes.
- Associated diseases and disorders
Hormone imbalances involving estradiol 17-beta can lead to various conditions. It is notably associated with breast cancer where abnormal levels and receptor engagement can stimulate tumor growth. Osteoporosis is another disorder linked to estrogen levels as estradiol 17-beta preserves bone density by modulating bone resorption. Additionally hormonal fluctuations involving related proteins like aromatase and sex hormone-binding globulin (SHBG) can exacerbate the progression of these diseases further linking estradiol 17-beta to critical health outcomes.
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2 product images
Competitive ELISA - 17 beta Estradiol ELISA Kit (ab108667)
Representative Standard Curve using ab108667
Competitive ELISA - 17 beta Estradiol ELISA Kit (ab108667)
17 beta Estradiol measured in biological fluids showing quantity (pg) per mL of tested sample. Samples were diluted 1-2 fold.
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