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20 HETE ELISA Kit is a Competitive ELISA kit for the measurement of 20 HETE in Plasma, Cell culture extracts, Tissue, Serum samples.

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Competitive ELISA - 20 HETE ELISA Kit (AB175817), expandable thumbnail

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Key facts

Detection method
Colorimetric
Sample types
Plasma, Cell culture extracts, Tissue, Serum
Assay type
Competitive
Range
0.01 - 1000 ng/mL
Sensitivity
= 10 pg/mL

Reactivity data

Application
cELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

What's included?

1 x 96 Tests
Components
1000X 20-HETE HRP Conjugates
1 x 12 µL
10X Sample Dilution Buffer
1 x 25 mL
10X Wash Buffer Solution
1 x 25 mL
20-HETE Standard
1 x 2 µL
HRP Buffer
1 x 15 mL
Pre-Coated 96-Well Microplate
1 x 1 Unit
TMB Substrate
1 x 24 mL

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20 HETE ELISA Kit is a Competitive ELISA kit for the measurement of 20 HETE in Plasma, Cell culture extracts, Tissue, Serum samples.

Key facts

Detection method
Colorimetric
Sample types
Plasma, Cell culture extracts, Tissue, Serum
Assay type
Competitive
Range
0.01 - 1000 ng/mL
Assay Platform
Microplate
Sensitivity
= 10 pg/mL

Precision

Intra assay

Sample
Overall
n
12
mean
-
SD
-
C.V.
= 0.3

Inter assay

Sample
Overall
n
12
mean
-
SD
-
C.V.
= 0.78

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Abcam's 20 HETE competitive in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the determination of 20 HETE (also known as 20-OH-AA) levels in biological samples. The specificity of the 20 HETE ELISA was investigated using authentic 20 HETE and a panel of fatty acids which, based on their structure, might be anticipated to compete with 20 HETE for binding to antibodies for 20 HETE. Anti-20 HETE did not cross-react with 14,15- and 11,12-DHETs, PGE2 and showed almost no cross-reactivity even with structurally extremely similar arachidonic acid (AA), linoleic acid and linolenic acid as shown in the competitive ELISA analysis. Considering the only difference between 20-HETE and AA is an oxygen molecule, the specificity of this kit is a surprise.

Human essential and salt-sensitive hypertensions were related to differential AA metabolism by cytochrome P450 (CYP) 4A which has AA-ω-hydroxylase (20 HETE synthesis) activity. Increased circulating insulin inhibits 20 HETE synthesis in obese hypertensive subjects.

Recently, CYP4F2 genetic variants, which increased urinary 20 HETE secretion, were found to be correlated with the risk for hypertension in a Chinese population.

This kit can be used for the determination of 20 HETE in serum, plasma, cells and tissues following proper isolation and purification.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

20-Hydroxyeicosatetraenoic acid (20-HETE) plays an important role in mechanism as a metabolite of arachidonic acid a polyunsaturated fatty acid. Cytochrome P450 enzymes especially those from the CYP4A and CYP4F families produce 20-HETE. This lipid molecule has a molecular mass of 320.5 g/mol. 20-HETE expresses widely in several tissues including the kidneys brain and blood vessels. Its synthesis occurs mainly in the smooth muscle cells and the renal tubular cells indicating its primary sites of action and function within the human body.

Biological function summary

20-HETE modulates various physiological processes. It functions independently rather than as part of a complex making it a unique regulator. In the cardiovascular system 20-HETE controls vascular tone by influencing the contraction and relaxation of blood vessels. It also affects renal function contributing significantly to sodium and water balance therefore impacting blood pressure regulation. Its ability to mediate cellular signaling cascades highlights its significance in maintaining homeostasis.

Pathways

20-HETE integrates into the renin-angiotensin system and the arachidonic acid metabolism pathway. In the renin-angiotensin system 20-HETE interacts with angiotensin II to potentiate vascular reactivity. In the arachidonic acid pathway 20-HETE is synthesized from arachidonic acid positioning it upstream from other bioactive eicosanoids. These interactions with proteins like angiotensin II and enzymes of the cytochrome P450 family highlight 20-HETE's role in diverse biological pathways that regulate vascular function and fluid homeostasis.

Associated diseases and disorders

20-HETE plays a role in essential hypertension and ischemic stroke. In essential hypertension elevated levels of 20-HETE contribute to increased vascular resistance and sodium retention promoting high blood pressure. Associations with ischemic stroke emerge through its impact on vascular tone and blood flow regulation. Researchers have noticed that alterations in cytochrome P450 enzyme activity which affects 20-HETE levels can result in cerebrovascular complications. These connections highlight the importance of 20-HETE in understanding and potentially treating cardiovascular diseases.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Competitive ELISA - 20 HETE ELISA Kit (ab175817), expandable thumbnail

    Competitive ELISA - 20 HETE ELISA Kit (ab175817)

    Example of standard curve obtained with ab175817.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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