AB175817

20 HETE ELISA Kit

Name: 20 HETE ELISA Kit (ab175817) | Abcam
Brand: Abcam Limited
SKU: AB175817

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(2 Publications)

20 HETE ELISA Kit is a Competitive ELISA for the measurement of 20 HETE in in Biofluids, Cell/Tissue Extracts samples.

1 Images

Competitive ELISA - 20 HETE ELISA Kit (AB175817)

cELISA

Supplier Data

Competitive ELISA - 20 HETE ELISA Kit (AB175817)

Example of standard curve obtained with ab175817.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture extracts, Tissue, Serum

Assay type

Competitive

Sensitivity

= 10 pg/mL

Range

0.01 - 1000 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's 20 HETE competitive in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the determination of 20 HETE (also known as 20-OH-AA) levels in biological samples. The specificity of the 20 HETE ELISA was investigated using authentic 20 HETE and a panel of fatty acids which, based on their structure, might be anticipated to compete with 20 HETE for binding to antibodies for 20 HETE. Anti-20 HETE did not cross-react with 14,15- and 11,12-DHETs, PGE2 and showed almost no cross-reactivity even with structurally extremely similar arachidonic acid (AA), linoleic acid and linolenic acid as shown in the competitive ELISA analysis. Considering the only difference between 20-HETE and AA is an oxygen molecule, the specificity of this kit is a surprise.

Human essential and salt-sensitive hypertensions were related to differential AA metabolism by cytochrome P450 (CYP) 4A which has AA-ω-hydroxylase (20 HETE synthesis) activity. Increased circulating insulin inhibits 20 HETE synthesis in obese hypertensive subjects.

Recently, CYP4F2 genetic variants, which increased urinary 20 HETE secretion, were found to be correlated with the risk for hypertension in a Chinese population.

This kit can be used for the determination of 20 HETE in serum, plasma, cells and tissues following proper isolation and purification.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 0.3" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 0.78" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab175817-1x96Tests", "assetComponentDetails": [ { "size":"1 x 22 mL", "name":"TMB Substrate", "number":"AB175817-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"HRP Buffer", "number":"AB175817-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"10X Sample Dilution Buffer", "number":"AB175817-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"10X Wash Buffer Solution", "number":"AB175817-CMP07", "productcode":"" }, { "size":"1 x 12 µL", "name":"1000X 20-HETE HRP Conjugates", "number":"AB175817-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-Coated 96-Well Microplate", "number":"AB175817-CMP01", "productcode":"" }, { "size":"1 x 2 µL", "name":"20-HETE Standard", "number":"AB175817-CMP03", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

20-Hydroxyeicosatetraenoic acid (20-HETE) plays an important role in mechanism as a metabolite of arachidonic acid a polyunsaturated fatty acid. Cytochrome P450 enzymes especially those from the CYP4A and CYP4F families produce 20-HETE. This lipid molecule has a molecular mass of 320.5 g/mol. 20-HETE expresses widely in several tissues including the kidneys brain and blood vessels. Its synthesis occurs mainly in the smooth muscle cells and the renal tubular cells indicating its primary sites of action and function within the human body.

Biological function summary

20-HETE modulates various physiological processes. It functions independently rather than as part of a complex making it a unique regulator. In the cardiovascular system 20-HETE controls vascular tone by influencing the contraction and relaxation of blood vessels. It also affects renal function contributing significantly to sodium and water balance therefore impacting blood pressure regulation. Its ability to mediate cellular signaling cascades highlights its significance in maintaining homeostasis.

Pathways

20-HETE integrates into the renin-angiotensin system and the arachidonic acid metabolism pathway. In the renin-angiotensin system 20-HETE interacts with angiotensin II to potentiate vascular reactivity. In the arachidonic acid pathway 20-HETE is synthesized from arachidonic acid positioning it upstream from other bioactive eicosanoids. These interactions with proteins like angiotensin II and enzymes of the cytochrome P450 family highlight 20-HETE's role in diverse biological pathways that regulate vascular function and fluid homeostasis.

20-HETE plays a role in essential hypertension and ischemic stroke. In essential hypertension elevated levels of 20-HETE contribute to increased vascular resistance and sodium retention promoting high blood pressure. Associations with ischemic stroke emerge through its impact on vascular tone and blood flow regulation. Researchers have noticed that alterations in cytochrome P450 enzyme activity which affects 20-HETE levels can result in cerebrovascular complications. These connections highlight the importance of 20-HETE in understanding and potentially treating cardiovascular diseases.

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Product protocols

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Target data

See full target information 20-Hydroxyeicosatetraenoic acid

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell proliferation 54:e12964 PubMed33314534

2020

20-HETE synthesis inhibition attenuates traumatic brain injury-induced mitochondrial dysfunction and neuronal apoptosis via the SIRT1/PGC-1α pathway: A translational study.

Applications

Unspecified application

Species

Unspecified reactive species

Wenxing Cui,Xun Wu,Yingwu Shi,Wei Guo,Jianing Luo,Haixiao Liu,Longlong Zheng,Yong Du,Ping Wang,Qiang Wang,Dayun Feng,Shunnan Ge,Yan Qu

International journal of biological sciences 15:2037-2050 PubMed31592228

2019

Vascular Protection of TPE-CA on Hyperhomocysteinemia-induced Vascular Endothelial Dysfunction through AA Metabolism Modulated CYPs Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Li,Zhenli Liu,Linlin Liu,Wen Li,Zhiwen Cao,Zhiqian Song,Qianqian Yang,Aiping Lu,Cheng Lu,Yuanyan Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

20 HETE ELISA Kit

Competitive ELISA - 20 HETE ELISA Kit (AB175817)

Key facts

Detection method

Sample types

Assay type

Sensitivity

Range

Assay Platform

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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