AB213966

25(OH) Vitamin D ELISA kit

Name: 25(OH) Vitamin D ELISA kit (ab213966) | Abcam
Brand: Abcam Limited
SKU: AB213966
Price: 730 USD
Availability: InStock

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(24 Publications)

The 25(OH) Vitamin D ELISA kit is a competitive ELISA designed to quantify 25-hydroxy vitamin D (vitamin D2 and vitamin D3) in serum and plasma with a sensitivity of 1.98 ng/mL.

- Wide dynamic range – quantifies 0.5–1010 ng/mL
- Assay platform – pre-coated microplate (12 x 8-well strips) for easy setup and reliable results
- Fast assay time – complete results in 1 hour 30 minutes
- Cited in over 15 publications

1 Images

ELISA - 25(OH) Vitamin D ELISA kit (AB213966)

ELISA

Supplier Data

ELISA - 25(OH) Vitamin D ELISA kit (AB213966)

25(OH) Vitamin D ELISA Kit (ab213966) Standard Curve

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Human

Assay type

Competitive

Sensitivity

= 1.98 ng/mL

Range

0.5 - 1010 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

25(OH) Vitamin D ELISA kit ab213966 is a competitive ELISA kit to measure 25-hydroxy vitamin D (vitamin D2 & vitamin D3) in human serum and plasma with a sensitivity of 1.98 ng/mL.

How the assay works

25(OH) Vitamin D ELISA kit ab213966 is a competitive ELISA. There are 2 types of competitive ELISAs:

antigen down: The sample antigen competes with a reference antigen for binding to a specific amount of labeled antibody. The reference antigen is pre-coated on a multi-well plate. The sample is added to the plate wells with a labeled antibody. Depending on the amount of antigen in the sample, more or fewer free antibodies will be available to bind the reference antigen. This means that the more of the antigen there is in the sample, the less reference antigen will be detected and the weaker the signal will be.
antibody down: the labeled antigen and the sample antigen (unlabeled) compete for binding to the primary antibody, which is immobilized on the plate. The lower the amount of antigen in the sample, the stronger the signal due to more labelled antigen in the well.

Assay specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility.

25(OH) Vitamin D ELISA kit ab213966 protocol summary

Add standards or samples to the wells, incubate 2 hrs
Wash, then add 50 µl of Biotinylated Antibody per well. Incubate 1 hr
Wash, then add 50 µl of SP Conjugate per well. Incubate 30 min
Wash, then add 50 µl of Chromogen Substrate per well. Incubate 20 min
Add 50 µl of Stop Solution per well. Read at 450 nm immediately

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 30, "mean": "297.2 ng/mL", "standardDeviation": null, "coefficientOfVariability": "11.5" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 30, "mean": "38.4 ng/mL", "standardDeviation": null, "coefficientOfVariability": "15.8" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 20, "mean": "225.8 ng/mL", "standardDeviation": null, "coefficientOfVariability": "1.6" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 20, "mean": "37.3 ng/mL", "standardDeviation": null, "coefficientOfVariability": "2.1" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 20, "mean": "5.3 ng/mL", "standardDeviation": null, "coefficientOfVariability": "3.4" } ]

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Recovery

[ { "sample": "Serum", "range": "84 - 97.9 %", "average": "= 91.9" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

25-OH Vitamin D3 also known as calcidiol or 25-hydroxycholecalciferol serves as a prohormone and the main storage form of vitamin D in the human body. It has a molecular mass of approximately 400.64 g/mol. The liver expresses it where the conversion from vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol) occurs. It circulates in the bloodstream and acts as a reservoir for the active form of vitamin D.

Biological function summary

25-hydroxyvitamin D3 is important to maintaining calcium and phosphate homeostasis. It is not part of a protein complex in its function as a circulatory prohormone. The kidneys convert it into the active form 125-dihydroxyvitamin D3 (calcitriol) which then interacts with the vitamin D receptor (VDR) to modulate gene expression related to calcium metabolism.

Pathways

This vitamin D3 metabolite holds importance in the calcium signaling and bone remodeling pathways. Its conversion to calcitriol aids in the regulation of parathyroid hormone (PTH) ensuring calcium balance in the bloodstream. The pathway includes elements such as the VDR and CYP27B1 which are necessary for its activation and action.

Alterations in 25-OH Vitamin D3 levels influence conditions like osteoporosis and rickets. Both conditions relate to disruptions in calcium homeostasis. Proteins like the VDR and CYP27B1 are involved in these disorders influencing bone density and mineralization through the modulation of calcidiol and calcitriol levels.

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Product protocols

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Target data

See full target information 25-OH Vitamin D3

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Publications (24)

Recent publications for all applications. Explore the full list and refine your search

Obstetrics & gynecology science 68:334-343 PubMed40534210

2025

Impact of ultraviolet radiation on bone density and muscle strength in postmenopausal women: a randomized controlled study.

Applications

Unspecified application

Species

Unspecified reactive species

Wafaa M Kamal,Ahmed M Maged,Noha Salah,Nour A El-Goly,Wael El-Sherbiny,Elham S Hassan

Frontiers in immunology 15:1466802 PubMed39840066

2025

Restoring natural killer cell activity in lung injury with 1,25-hydroxy vitamin D: a promising therapeutic approach.

Applications

Unspecified application

Species

Unspecified reactive species

Johnny Amer,Ahmad Salhab,Mohammad Abuawad

Pakistan journal of medical sciences 40:2363-2367 PubMed39554665

2024

Key predictors of fertility: Exploring the role of Vitamin-D.

Applications

Unspecified application

Species

Unspecified reactive species

Huma Salahuddin,Samar Zaki,Mussarat Ashraf,Rehana Rehman

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 38:e70135 PubMed39467145

2024

Retinal G-protein-coupled receptor deletion exacerbates AMD-like changes via the PINK1-parkin pathway under oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Guo,Sitong Chen,Wenxue Guan,Ningda Xu,Li Zhu,Wei Du,Zhiming Liu,Henry K W Fong,Lvzhen Huang,Mingwei Zhao

PloS one 19:e0309610 PubMed39432457

2024

The effectiveness of vitamin D supplementation in patients with end-stage knee osteoarthritis: Study protocol for a double-blinded, randomized controlled trial.

Applications

Unspecified application

Species

Unspecified reactive species

Qian-Wen Wang,Michael Tim-Yun Ong,Gene Chi-Wai Man,Yi-Man Yeung,Xin He,Ben Chi-Yin Choi,Jonathan Patrick Ng,Daniel Kam-Wah Mok,Tsz-Ping Lam,Patrick Shu-Hang Yung

Scientific reports 14:24313 PubMed39414885

2024

SARS-CoV-2 infection causes a decline in renal megalin expression and affects vitamin D metabolism in the kidney of K18-hACE2 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshifumi Kurosaki,Toshihide Matsumoto,Takayuki Uematsu,Fumitaka Kawakami,Rei Kawashima,Shun Tamaki,Motoki Imai,Takafumi Ichikawa,Naohito Ishii,Hidero Kitasato,Hideaki Hanaki,Makoto Kubo

Asia-Pacific journal of sports medicine, arthroscopy, rehabilitation and technology 35:76-80 PubMed38292822

2024

Serum vitamin D insufficiency is correlated with quadriceps neuromuscular functions in patients with anterior cruciate ligament injury: A preliminary study.

Applications

Unspecified application

Species

Unspecified reactive species

Jihong Qiu,Chi-Yin Choi,Gene Chi-Wai Man,Xin He,Mingqian Yu,Mingde Cao,Qianwen Wang,Jonathan Patrick Ng,Patrick Shu-Hang Yung,Michael Tim-Yun Ong

The journal of physiological sciences : JPS 73:29 PubMed37964189

2023

Relationship between the thrombospondin-1/Toll-like receptor 4 (TSP1/TLR4) pathway and vitamin D levels in obese and normal weight subjects with different metabolic phenotypes.

Applications

Unspecified application

Species

Unspecified reactive species

Eman Y Khairy,Azza Saad

Journal of orthopaedic surgery and research 18:778 PubMed37845656

2023

Comparison between calcaneus quantitative ultrasound and the gold standard DXA in the ability to detect osteoporosis in chronic obstructive pulmonary disease patients.

Applications

Unspecified application

Species

Unspecified reactive species

Wandee Chanprasertpinyo,Chuchard Punsawad,Rapheeporn Khwanchuea,Naparat Sukkriang,Pirada Yincharoen,Chaiwat Rerkswattavorn

PloS one 18:e0287727 PubMed37428803

2023

SIRT1 and antioxidants in infertile females: Exploration of the role of vitamin D.

Applications

Unspecified application

Species

Unspecified reactive species

Faiza Alam,Maheen Shahid,Sumaira Riffat,Ihsan Nazurah Zulkipli,Fatima Syed,Mussarat Ashraf,Rehana Rehman

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

25(OH) Vitamin D ELISA kit

ELISA - 25(OH) Vitamin D ELISA kit (AB213966)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (24)

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Secondary Antibodies

Primary Antibodies

Assay Kits

Product promise