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3-Nitrotyrosine ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of 3-Nitrotyrosine in Cell culture extracts, Tissue Extracts samples.


Images

Sandwich ELISA - 3-Nitrotyrosine ELISA Kit (AB116691), expandable thumbnail
  • Biochemical assay - 3-Nitrotyrosine ELISA Kit (AB116691), expandable thumbnail

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Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Assay type

Sandwich (quantitative)

Range

8 - 1000 ng/mL

Sensitivity

= 8 ng/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

What's included?

1 x 96 Tests
Components
10X 3-nitrotyrosine Detector Antibody
1 x 1 mL
10X Blocking Buffer
1 x 6 mL
10X HRP Label
1 x 1 mL
20X Buffer
1 x 20 mL
3-nitrotyrosine BSA standard (Lyophilized)
1 x 1 Vial
Extraction Buffer (ab260490)
1 x 15 mL
HRP Development Solution
1 x 12 mL
Microplate 96 antibody coated wells in 12 strips
1 x 1 Unit

Recommended products

3-Nitrotyrosine ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of 3-Nitrotyrosine in Cell culture extracts, Tissue Extracts samples.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Assay type

Sandwich (quantitative)

Range

8 - 1000 ng/mL

Assay Platform

Microplate

Sensitivity

= 8 ng/mL

Precision

Intra assay

Sample

Sample 1

n

8

mean

-

SD

-

C.V.

6.9

Inter assay

Sample

Sample 1

n

3

mean

-

SD

-

C.V.

13

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

ab116691 is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of 3-nitrotyrosine in cell and tissue lysates. The assay employs an antibody specific for 3-nitrotyrosine coated on a 96-well plate. Standards and samples are pipetted into the wells and 3-nitrotyrosine present in the sample is bound to the wells by the immobilized antibody. The wells are washed and a biotin labeled anti-3-nitrotyrosine detector antibody is added. After washing away unbound detector antibody, HRP-conjugated streptavidin specific for the biotin labeled detector antibody is pipetted into the wells. The wells are again washed, an HRP substrate solution (TMB) is added to the wells and color develops in proportion to the amount of 3-nitrotyrosine bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.

Store all components at 4°C. This kit is stable for 6 months from receipt. After reconstitution the standard should be stored at -80°C. Unused microplate strips should be returned to the pouch containing the desiccant and resealed.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Nitrotyrosine also known as 3-nitrotyrosine is a marker for nitrosative stress and oxidative damage. It comes from the nitration of tyrosine an amino acid primarily through the action of reactive nitrogen species. Nitrotyrosine can be found in proteins and its molecular mass varies depending on the protein context. It is present across various tissues and fluid samples within the body particularly where high oxidative stress occurs.

Biological function summary

Nitrotyrosine acts as an indicator of cellular damage and protein modification due to stress conditions. When proteins have nitrotyrosine residues their function might change which can lead to cellular signaling disruption. Nitrotyrosine is not usually part of a specific complex but it affects proteins and enzymes by altering their activity and stability contributing to larger stress response mechanisms.

Pathways

Proteins modified by nitrotyrosine are involved in cellular pathways related to oxidative stress and inflammation. Particularly pathways like the nitric oxide synthase pathway and the inflammatory response pathway include nitrotyrosine-modified proteins. It is related to proteins such as nitric oxide synthase (NOS) and superoxide dismutase (SOD) important in managing oxidative balance and stress responses in cells.

Associated diseases and disorders

Nitrotyrosine is significantly linked to diseases related to oxidative stress such as cardiovascular disease and neurodegenerative disorders. These conditions often have increased levels of nitrotyrosine indicating heightened oxidative damage. Through these diseases nitrotyrosine connects with proteins like amyloid-beta in neurodegenerative disorders and endothelial nitric oxide synthase (eNOS) in cardiovascular diseases reflecting the impact of oxidative stress on these proteins.

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2 product images

  • Sandwich ELISA - 3-Nitrotyrosine ELISA Kit (ab116691), expandable thumbnail

    Sandwich ELISA - 3-Nitrotyrosine ELISA Kit (ab116691)

    Example standard curve.

  • Biochemical assay - 3-Nitrotyrosine ELISA Kit (ab116691), expandable thumbnail

    Biochemical assay - 3-Nitrotyrosine ELISA Kit (ab116691)

    Chen et al used Lipid Peroxidation (MDA) Assay Kit Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric) ab118970, 3-Nitrotyrosine Kit ab116691, and 8-OHdG ELISA kit 8-hydroxy 2 deoxyguanosine ELISA Kit ab201734 to investigate oxidative stress in spinal cord injury in mice.

    FGF5 overexpression reduces inflammation and oxidative stress in mice treated with surgery to create spinal cord injury. Yellow = control. Green = FGF5 overexpression. Mice were overexpressed with FGF5 using lentiviral vectors, and then were exposed to SCI or sham surgery 2 weeks post‐injection. The levels of MDA, 3‐NT and 8‐OHdG. n = 6 for each groups. Data are expressed as the mean ± standard deviation and p < 0.05 was considered statistically significant. *p < 0.05.

    Levels in the spinal cord of malondialdehyde (MDA), 3‐nitrotyrosine (3‐NT) and 8‐hydroxy 2 deoxyguanosine (8‐OHdG) were detected using commercial kits (#Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric) ab118970 for MDA, #ab116691 for 3‐NT, #8-hydroxy 2 deoxyguanosine ELISA Kit ab201734 for 8‐OHdG; Abcam) to evaluate oxidative damage of lipids, proteins and nucleic acids according to the manufacturer's instructions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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