AB315264

3-Nitrotyrosine ELISA Kit - Extracellular

SimpleStep
What is this?

Be the first to review this product! Submit a review

(0 Publication)

3-Nitrotyrosine ELISA Kit - Extracellular is a single-wash 90-min Simplestep used to quantify 3-Nitrotyrosine with a sensitivity of 41 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Competitive ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders

4 Images

Competitive ELISA - 3-Nitrotyrosine ELISA Kit - Extracellular (AB315264)

cELISA

Supplier Data

Competitive ELISA - 3-Nitrotyrosine ELISA Kit - Extracellular (AB315264)

Serum of ten individual healthy Human donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean 3-Nitrotyrosine concentration of detected samples was determined to be 183 ng/mL with a range of undetected – 500.7 ng/mL.

cELISA

Supplier Data

Competitive ELISA - 3-Nitrotyrosine ELISA Kit - Extracellular (AB315264)

Linearity of dilution.

Interpolated concentration of native 3-Nitrotyrosine was measured in duplicate at different sample concentrations. Undiluted samples are 50% urine. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

cELISA

Supplier Data

Competitive ELISA - 3-Nitrotyrosine ELISA Kit - Extracellular (AB315264)

Linearity of dilution.

Recombinant 3-Nitrotyrosine was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 50% Human serum, plasma (citrate), plasma (EDTA), plasma (heparin), and 25% urine. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

cELISA

Supplier Data

Competitive ELISA - 3-Nitrotyrosine ELISA Kit - Extracellular (AB315264)

Example of 3-Nitrotyrosine standard curve. OD (450 nM) values (mean +/- SD) are graphed

Key facts

Detection method

Colorimetric

Sample types

Serum, Heparin Plasma, Citrate plasma, EDTA Plasma, Urine

Assay type

Competitive

Results type

Quantitative

Sensitivity

= 41 ng/mL

Range

62.5 - 4000 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

3-Nitrotyrosine SimpleStep ELISA® kit is a single-wash 90 min competitive ELISA designed for the quantitative measurement of 3-Nitrotyrosine in Serum, Hep Plasma, Cit Plasma, EDTA Plasma, Urine. Quantitate 3-Nitrotyrosine with 41 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture conjugates conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to competitive ELISA allows the formation of the antibody-analyte complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

Single-wash protocol reduces assay time to 90 minutes or less
High sensitivity, specificity and reproducibility from superior antibodies
Fully validated in biological samples
96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

style

left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 9.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 9.4" } ]

style

left-column

Recovery

[ { "sample": "Serum", "range": "80 - 89 %", "average": "= 84" }, { "sample": "Heparin Plasma", "range": "88 - 112 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "83 - 111 %", "average": "= 99" }, { "sample": "EDTA Plasma", "range": "81 - 100 %", "average": "= 91" }, { "sample": "Urine", "range": "85 - 125 %", "average": "= 101" } ]

style

left-column

What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab315264-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB315264-CMP07" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB315264-CMP08" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB315264-CMP09" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB315264-CMP10" }, { "size":"10 x 8 mL", "name":"Antibody Diluent 4BI", "number":"AB315264-CMP04" }, { "size":"10 x 80 µL", "name":"50X Nitrotyrosine HRP Conjugate", "number":"AB315264-CMP02" }, { "size":"10 x 80 µL", "name":"50X Nitrotyrosine CaptSure Conjugate", "number":"AB315264-CMP01" }, { "size":"20 x 1 Vial", "name":"3-Nitrotyrosine Lyophilized Standard", "number":"AB315264-CMP03" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB315264-CMP05" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB315264-CMP06" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab315264-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB315264-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB315264-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB315264-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB315264-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB315264-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"3-Nitrotyrosine Lyophilized Standard", "number":"AB315264-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Antibody Diluent 4BI", "number":"AB315264-CMP04", "productcode":"" }, { "size":"1 x 80 µL", "name":"50X Nitrotyrosine HRP Conjugate", "number":"AB315264-CMP02", "productcode":"" }, { "size":"1 x 80 µL", "name":"50X Nitrotyrosine CaptSure Conjugate", "number":"AB315264-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB315264-CMP05", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

3-Nitrotyrosine forms when peroxynitrite interacts with tyrosine residues in proteins. It is a marker of nitrosative stress and oxidative damage with the molecular mass of the modified residue remaining similar to that of tyrosine. This molecule is commonly found in conditions with high oxidative stress such as inflammation and is present in tissues with significant reactive nitrogen species. 3-Nitrotyrosine detection is facilitated by various techniques including nitrotyrosine staining and plays a notable role in the study of oxidative stress markers.

Biological function summary

Modification of tyrosine can affect protein function and cell signaling. This post-translational modification can hinder protein function or disrupt protein-protein interactions altering cellular processes. Nitrotyrosine often forms part of the cellular response to oxidative damage contributing to complex antioxidant defenses. The presence of nitrotyrosine indicates a cellular environment stressed by reactive nitrogen species linking it to pathophysiological processes in cells undergoing such stress.

Pathways

3-nitrotyrosine acts in oxidative stress and cell signaling routes. It integrates into pathways related to the cellular stress response particularly the antioxidant defense mechanisms. Proteins such as superoxide dismutase (SOD) may indirectly relate to nitrotyrosine since they mitigate reactive species trying to maintain redox balance. The presence of nitrotyrosine can indicate excess stress on these systems reflecting shifts in signaling that affect cell survival.

High levels of 3-nitrotyrosine associate with cardiovascular diseases and neurodegenerative disorders. In diseases such as atherosclerosis and Alzheimer's 3-nitrotyrosine presence indicates elevated oxidative stress. Through these conditions it relates to proteins like amyloid-beta in Alzheimer's disease or low-density lipoprotein (LDL) in cardiovascular disease. These associations emphasize the potential of 3-nitrotyrosine as a biomarker for oxidative damage in stress-related diseases.

style

left-column

Product protocols

style

left-column

Target data

See full target information 3-Nitrotyrosine

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com