4-Hydroxynonenal ELISA Kit

Product details

4-Hydroxynonenal ELISA Kit is a competitive ELISA kit to measure 4-HNE in plasma, tissue Homogenate, serum, other biological fluids with a sensitivity of 18.75 pg/mL.

How the assay works

The concentration of the 4-HNE in the samples can determined by comparing the optical density to the standard curve. The OD is inversely proportional to the amount of 4-HNE captured from the samples.

Assay Specificity

No significant cross-reactivity or interference between 4-HNE and analogues was observed.

4-Hydroxynonenal ELISA Kit ab287803 protocol summary:

1. Wash plate 2 times before adding standard, sample and control wells
2. Add each standards or samples into appropriate wells. Immediately add Biotin-labeled Antibody Working Solution into each well.
3. Incubate for 45 minutes at 37°C.
4. Wash
5. Add SABC working solution into each well, cover the plate and incubate at 37ºC for 30 min
6. Wash
7. Add TMB substrate into each well, cover the plate and incubate at 37 ºC in dark within 15-30 min.
8. Add Stop solution and read results immediately at 450 nm.

How other researchers are using 4-Hydroxynonenal ELISA Kit ab287803

4-hydroxynonenal (4-HNE) is used as a marker of lipid peroxydation. It is also in higher quantities during oxidative stress due to the increase in the lipid peroxidation chain reaction. It exhibits various biological activities such as cytotoxicity, growth inhibiting activity, genotoxicity, and chemotactic activity. It also inhibits Sirtuin 3 (SIRT3) via thiol-specific modification

This product is manufactured by BioVision, an Abcam company and was previously called E4645 4-Hydroxynonenal (4-HNE) ELISA Kit. E4645-100 is the same size as the 96 test size of ab287803.