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AB133023

6-keto-PGF1 alpha ELISA Kit

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(2 Publications)

6-keto-PGF1 alpha ELISA Kit is a Competitive ELISA for the measurement of 6-keto-PGF1 alpha in Human in Biofluids, Cell Culture Media samples.
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Competitive Binding Assays - 6-keto-PGF1 alpha ELISA Kit (AB133023)
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Supplier Data

Competitive Binding Assays - 6-keto-PGF1 alpha ELISA Kit (AB133023)

Representative Standard Curve using ab133023.

Competitive Binding Assays - 6-keto-PGF1 alpha ELISA Kit (AB133023)
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Lab

Competitive Binding Assays - 6-keto-PGF1 alpha ELISA Kit (AB133023)

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Plasma, Cell culture supernatant, Serum, Tissue Culture Media

Reacts with

Human

Assay type

Competitive

Sensitivity

= 1.4 pg/mL

Range

3.2 - 50000 pg/mL

Assay time

3h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's 6-keto-PGF1 alpha in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 6-keto-PGF1 alpha in serum, urine, tissue culture media and other biological fluids.

A donkey anti-sheep IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-6-keto-PGF1 alpha antigen and a polyclonal sheep antibody specific to 6-keto-PGF1 alpha. After incubation the excess reagents are washed away. pNpp substrate is added. After a short incubation the enzyme reaction is stopped and the yellow color generated by alkaline phosphatase is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of 6-keto-PGF1α captured in the plate.

6-keto-PGF1α is a stable hydrolysis product of prostacyclin (PGI2). Due to the short half-life of PGI2 in buffer (2-3 minutes) the production of prostacyclin is typically monitored by measurement of the 6-keto-PGF1α metabolite and its derivative in urine, 2,3-dinor-6-keto-PGF1α. PGI2 has autocoid functions that include platelet aggregation, inhibition of vasoconstrictor responses to pressor hormones, platelet anti- and disaggregatory properties, and reproductive functions. The effects are induced by receptor-mediated elevation of cAMP. Prostacyclin has clinical applications in atherosclerosis and protection from myocardial ischemia.

Cross Reactivity

Compound Cross Reactivity
6-keto-PGF1α 100%
2,3-dinor-6-keto-PGF1α 3.17%
PGF2α 1.67%
PGD2 0.60%
PGF1α 0.60%
PGE1 0.20%
6,15-diketo-13,14-dihydro-PGF1α 0.12%
13,14-dihydro-15-keto-PGF1α <0.01%
15-keto-PGF2α <0.01%
PGA2 <0.01%
PGB1 <0.01%
PGE2 <0.01%
Thromboxane B2 <0.01%
15-HpETE <0.01%
2-Arachidonoylglycerol <0.01%
Anandamide <0.01%
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Precision

[ { "reproducibilityType": "Inter", "sample": "High", "replicates": 8, "mean": "4828 pg/mL", "standardDeviation": null, "coefficientOfVariability": "6" }, { "reproducibilityType": "Inter", "sample": "Low conc", "replicates": 8, "mean": "239 pg/mL", "standardDeviation": null, "coefficientOfVariability": "7.9" }, { "reproducibilityType": "Inter", "sample": "Medium conc", "replicates": 8, "mean": "1220 pg/mL", "standardDeviation": null, "coefficientOfVariability": "5.2" }, { "reproducibilityType": "Intra", "sample": "High", "replicates": 10, "mean": "4886 pg/mL", "standardDeviation": null, "coefficientOfVariability": "2.9" }, { "reproducibilityType": "Intra", "sample": "Low conc", "replicates": 10, "mean": "41 pg/mL", "standardDeviation": null, "coefficientOfVariability": "13.1" }, { "reproducibilityType": "Intra", "sample": "Medium conc", "replicates": 10, "mean": "240 pg/mL", "standardDeviation": null, "coefficientOfVariability": "4.8" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": "= 108.4" }, { "sample": "Tissue Culture Media", "range": null, "average": "= 106.7" }, { "sample": "Urine", "range": null, "average": "= 108.7" }, { "sample": "Saliva", "range": null, "average": "= 110.7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab133023-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Sealer", "number":"AB133023-CMP02", "productcode":"" }, { "size":"1 x 27 mL", "name":"20X Wash Buffer Concentrate", "number":"AB133023-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Donkey anti-sheep IgG Microplate (12 x 8 wells)", "number":"AB133023-CMP05", "productcode":"" }, { "size":"1 x 5 mL", "name":"Stop Solution", "number":"AB133023-CMP04", "productcode":"" }, { "size":"1 x 27 mL", "name":"Assay Buffer", "number":"AB133023-CMP01", "productcode":"" }, { "size":"1 x 500 µL", "name":"6-keto-PGF1 alpha Standard", "number":"AB133023-CMP08", "productcode":"" }, { "size":"1 x 5 mL", "name":"6-keto-PGF1 alpha Alkaline Phosphatase Conjugate", "number":"AB133023-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"pNpp Substrate", "number":"AB133023-CMP03", "productcode":"" }, { "size":"1 x 5 mL", "name":"6-keto-PGF1 alpha EIA Antibody", "number":"AB133023-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

6-keto-PGF1α also known as 6-keto-prostaglandin F1α or simply PGF1α is a stable metabolite of prostacyclin (PGI2). Prostacyclin and its metabolites have a molecular mass of around 375 daltons. This metabolite predominantly arises from endothelial cells where it serves as an indicator of prostacyclin production. The 6-keto-PGF1α is produced following the spontaneous hydrolysis of PGI2 which is notoriously unstable in biological systems.
Biological function summary

This metabolite acts as an important marker in various physiological and pathological processes rather than having a direct biological activity. It forms part of a larger complex of prostanoids that includes other derivatives of arachidonic acid. As a breakdown product 6-keto-PGF1α does not directly engage in extensive signaling but reflects the prior synthesis and action of prostacyclin. Monitoring levels of this metabolite can provide valuable insights into the regulation and disturbances of prostacyclin pathways.

Pathways

Prostacyclin and its stable metabolite 6-keto-PGF1α play roles in the cardiovascular system by signaling within the arachidonic acid cascade. This pathway includes enzymes such as cyclooxygenase 1 and 2 (COX-1 and COX-2) and further conversion processes leading to various prostanoids. These pathways integrate with others involved in the regulation of blood flow inflammation and thrombosis with proteins like thromboxane A2 acting in opposition to prostacyclin balancing vasodilation and platelet aggregation.

6-keto-PGF1α levels are studied in conditions related to cardiovascular health such as hypertension and atherosclerosis. These conditions involve endothelial dysfunction where reduced production of prostacyclin compromises vascular homeostasis. This metabolite also relates to inflammatory disorders as disturbed prostaglandin production influences inflammation and blood vessel function. In both cases its presence pairs with other key metabolites like thromboxane A2 that offer insight into the disease mechanisms and potential therapeutic targets.
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Product protocols

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Target data

See full target information 6-keto-PGF1a
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceutical biology 60:175-184 PubMed35014931

2022

The antithrombosis effect of dehydroandrographolide succinate: and studies.

Applications

Unspecified application

Species

Unspecified reactive species

Bowen Yin,Shuhua Zhang,Yuxi Huang,Yuanzhu Long,Yiguo Chen,Shiyun Zhao,Aiqun Zhou,Minghua Cao,Xiaoming Yin,Daya Luo

Kidney international 88:796-803 PubMed26108065

2015

Antithrombin III/SerpinC1 insufficiency exacerbates renal ischemia/reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Wang,Guangyuan Zhang,Zeyuan Lu,Aron M Geurts,Kristie Usa,Howard J Jacob,Allen W Cowley,Niansong Wang,Mingyu Liang
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