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AB285254

8-hydroxy-2'-deoxyguanosine (8-OHdG) ELISA Kit

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(9 Publications)

8-hydroxy-2'-deoxyguanosine (8-OHdG) ELISA Kit is a competitive ELISA designed to quantify 8-hydroxy-2'-deoxyguanosine (8-OHdG) with a sensitivity of 0.94 ng/mL

- Colorimetric competitive ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 1.563 - 100 ng/ml
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Functional Studies - 8-hydroxy-2'-deoxyguanosine (8-OHdG) ELISA Kit (AB285254)
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Supplier Data

Functional Studies - 8-hydroxy-2'-deoxyguanosine (8-OHdG) ELISA Kit (AB285254)

Typical Standard Curve : These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Sample types

Plasma, Tissue Homogenate, Serum, Other biological fluids

Assay type

Competitive

Results type

Quantitative

Sensitivity

< 0.94 ng/mL

Range

1.563 - 100 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

8-hydroxy-2'-deoxyguanosine (8-OHdG) ELISA Kit (ab285254, K4160) is used for quantitative measurement of 8-OHdG in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.

This product is manufactured by BioVision, an Abcam company and was previously called K4160 8-hydroxy-2'-deoxyguanosine (8-OHdG) ELISA Kit. K4160-100 is the same size as the 1 x 96 tests size of ab285254.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

8-Hydroxy-2'-deoxyguanosine (8-OHdG) also known as 8-hydroxyguanine or simply 8-OHdG is a well-known marker of oxidative DNA damage. This compound forms through the oxidative modification of deoxyguanosine a nucleoside component of DNA mainly by reactive oxygen species (ROS). 8-OHdG is a stable product with a molecular mass of 283.26 g/mol. It is found in various tissues and is excreted in urine which makes it a useful non-invasive biomarker for assessing oxidative stress levels in the body.
Biological function summary

8-hydroxy-2-deoxyguanosine plays a significant role in indicating cellular oxidative damage but it doesn't act as a signaling molecule or enzyme. Instead it serves as a biomarker that results from the damage. Oxidative stress caused by reactive oxygen species can lead to the formation of 8-OHdG and indicates potential mutations that may arise if the damage goes unrepaired. Its formation does not involve complex protein assemblies but results from the attack on DNA by ROS highlighting cellular environments exposed to oxidative conditions.

Pathways

Several factors cause oxidative stress leading to the generation of 8-OHdG linking it to key biological pathways such as the DNA damage response and repair pathways. The base excision repair (BER) pathway is important for recognizing and repairing the damage introduced by 8-OHdG. Proteins such as 8-oxoguanine glycosylase (OGG1) directly interact with 8-OHdG during the repair process excising the damaged base from DNA to prevent mutations and maintain genomic integrity.

Researchers associate elevated levels of 8-OHdG with various conditions linked to oxidative stress such as cancer and neurodegenerative diseases like Alzheimer's disease. In cancer the accumulation of 8-OHdG can provide evidence of increased oxidative DNA damage which may lead to mutations and carcinogenesis. Alzheimer’s disease patients often exhibit elevated 8-OHdG levels indicating oxidative damage in neural tissues. The protein OGG1 plays a pivotal role in relationship with these diseases serving as an important enzyme for repairing the 8-OHdG lesions and potentially impacting disease progression.
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Product protocols

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Target data

See full target information 8-Hydroxy-2'-deoxyguanosine
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Life (Basel, Switzerland) 15: PubMed40003585

2025

Investigation of the Effect of Camellia Sinensis Essence Cream on Skin Burns.

Applications

Unspecified application

Species

Unspecified reactive species

Tolga Mercantepe,Adnan Yilmaz,Atilla Topcu,Ali Bilgin,Levent Tumkaya,Filiz Mercantepe

Dentistry journal 12: PubMed39727459

2024

Minimizing Oxidative Stress in Oral Surgery: A Comparative Study of Laser-Assisted and Conventional Third Molar Extractions.

Applications

Unspecified application

Species

Unspecified reactive species

Paul Șerban Popa,Elisabeta Claudia Popa-Cazacu,Anamaria Zaharescu,Gabriel Valeriu Popa,Mădălina Nicoleta Matei

European journal of histochemistry : EJH 68: PubMed39494460

2024

Alarin regulates RyR2 and SERCA2 to improve cardiac function in heart failure with preserved ejection fraction.

Applications

Unspecified application

Species

Unspecified reactive species

Jinshuang Li,Dawei Xu,Ce Shi,Chunqi Cheng,Ziheng Xu,Xingjuan Gao,Yong Cheng

Brain and behavior 14:e70039 PubMed39295108

2024

HIF-1α knockdown attenuates inflammation and oxidative stress in ischemic stroke male rats via CXCR4/NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Gao Chen,Xi Wang,Zhan Jin,Gao-Bo Hu,Qi-Hui Yu,Hai-Yan Jiang

The international journal of neuropsychopharmacology 27: PubMed38938182

2024

Thrombomodulin Improves Cognitive Deficits in Heat-Stressed Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Hsien Lin,Ling-Yu Tang,Lin-Yu Wang,Ching-Ping Chang

Cell stress & chaperones 28:801-809 PubMed37608231

2023

Aucubin promotes activation of AMPK and alleviates cerebral ischemia/reperfusion injury in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jin-Jing Zhao,Bo Zhao,Xiao Bai,Shuang Zhang,Rui Xu

Experimental and therapeutic medicine 25:265 PubMed37206558

2023

Sulforaphane alleviates lung ischemia‑reperfusion injury through activating Nrf‑2/HO‑1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Zhang,Shuxian Wang,Ying Zhang,Fenghuan Li,Chaoxiao Yu

Biomedicines 11: PubMed36979652

2023

Discriminating Different Bladder and Bladder Outlet Dysfunctions by Urinary Biomarkers in Women with Frequency-Urgency Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Fong Jhang,Yuan-Hong Jiang,Hann-Chorng Kuo

International journal of molecular sciences 23: PubMed36077523

2022

Telomere Shortening and Increased Oxidative Stress in Lumbar Disc Degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Manassanan Jitjumnong,Pornpan Chalermkitpanit,Tanyawan Suantawee,Sinsuda Dechsupa,Ladawan Vajarintarangoon,Sittisak Honsawek
View all publications
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