8-hydroxy 2 deoxyguanosine ELISA Kit ab201734 is used to quantify 8-hydroxy 2 deoxyguanosine with a sensitivity of 30 pg/ml.
Competitive ELISA Kit
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- Biomolecules & therapeutics 32:349-3602024Morroniside Protects C2C12 Myoblasts from Oxidative Damage Caused by ROS-Mediated Mitochondrial Damage and Induction of Endoplasmic Reticulum Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyun Hwangbo,Cheol Park,EunJin Bang,Hyuk Soon Kim,Sung-Jin Bae,Eunjeong Kim,Youngmi Jung,Sun-Hee Leem,Young Rok Seo,Su Hyun Hong,Gi-Young Kim,Jin Won Hyun,Yung Hyun ChoiPubMed 38602043
- Current issues in molecular biology 46:1593-16062024Plastoquinone-Derivative SkQ1 Improved the Biliary Intraepithelial Neoplasia during Liver Fluke Infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOxana Zaparina,Anna Kovner,Viktoria Petrova,Nataliya Kolosova,Viatcheslav Mordvinov,Maria PakharukovaPubMed 38392221
- NPJ Parkinson's disease 9:1662023Impaired neuron differentiation in GBA-associated Parkinson's disease is linked to cell cycle defects in organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIsabel Rosety,Alise Zagare,Claudia Saraiva,Sarah Nickels,Paul Antony,Catarina Almeida,Enrico Glaab,Rashi Halder,Sergiy Velychko,Thomas Rauen,Hans R Schöler,Silvia Bolognin,Thomas Sauter,Javier Jarazo,Rejko Krüger,Jens C SchwambornPubMed 38110400
- Journal of cellular and molecular medicine 27:3706-37162023Fibroblast growth factor 5 protects against spinal cord injury through activating AMPK pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng Chen,Bing-Rui Xiong,Shu-Yue Xian,Jing Zhang,Rui-Wen Ding,Ming Xu,Zong-Ze ZhangPubMed 37950418
- Antioxidants (Basel, Switzerland) 12:2023Effect of Particulate Matter 2.5 on Fetal Growth in Male and Preterm Infants through Oxidative Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSunwha Park,Eunjin Kwon,Gain Lee,Young-Ah You,Soo Min Kim,Young Min Hur,Sooyoung Jung,Yongho Jee,Mi Hye Park,Sung Hun Na,Young-Han Kim,Geum Joon Cho,Jin-Gon Bae,Soo-Jeong Lee,Sun Hwa Lee,Young Ju KimPubMed 38001768
- Biology open 12:2023Impact of high-fat diet and exposure to constant light on reproductive competence of female ICR mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKelsey Teeple,Prabha Rajput,Sara Scinto,Jenna Schoonmaker,Corrin Davis,Michayla Dinn,Mackenzie McIntosh,Sairam Krishnamurthy,Karen Plaut,Theresa CaseyPubMed 37843404
- Journal of translational medicine 21:6672023Tumor microenvironment in a minipig model of spinal cord glioma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMuhibullah S Tora,Stewart G Neill,Yuliya Lakhina,Hemza Assed,Michelle Zhang,Purva P Nagarajan,Thais Federici,Juanmarco Gutierrez,Kimberly B Hoang,Yuhong Du,Kecheng Lei,Nicholas M BoulisPubMed 37752585
- Life (Basel, Switzerland) 13:2023Impact of Particulate Matter Exposure and Aerobic Exercise on Circulating Biomarkers of Oxidative Stress, Antioxidant Status, and Inflammation in Young and Aged Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSu-Youn Cho,Hee-Tae RohPubMed 37895334
- Acta pharmaceutica Sinica. B 13:4477-45012023Miriplatin-loaded liposome, as a novel mitophagy inducer, suppresses pancreatic cancer proliferation through blocking POLG and TFAM-mediated mtDNA replication.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaowei Wang,Mengyan Wang,Meilian Cai,Rongguang Shao,Guimin Xia,Wuli ZhaoPubMed 37969736
- Biomedicines 11:2023Biosynthesized Selenium Nanoparticles Using Epigallocatechin Gallate Protect against Pentylenetetrazole-Induced Acute Epileptic Seizures in Mice via Antioxidative, Anti-Inflammatory, and Anti-Apoptotic Activities.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBarakat M Alrashdi,Alaa Fehaid,Rami B Kassab,Sara Rizk,Ola A Habotta,Ahmed E Abdel MoneimPubMed 37509594
Key facts
- Detection method
Colorimetric
- Sample types
Saliva, Urine, Plasma, Tissue Extracts, Serum, Cell Lysate
- Assay type
Competitive
- Range
0.94 - 60 ng/mL
- Assay time
2h
- Sensitivity
= 0.59 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application cELISA | Reactivity Reacts | Dilution info - | Notes - |
Alternative names
8-OH-dG
What's included?
Recommended products
8-hydroxy 2 deoxyguanosine ELISA Kit ab201734 is used to quantify 8-hydroxy 2 deoxyguanosine with a sensitivity of 30 pg/ml.
Competitive ELISA Kit
Key facts
- Detection method
Colorimetric
- Sample types
Saliva, Urine, Plasma, Tissue Extracts, Serum, Cell Lysate
- Assay type
Competitive
- Range
0.94 - 60 ng/mL
- Assay time
2h
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 0.59 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Spike | n 90 | mean - | SD - | C.V. < 5 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Spike | n 90 | mean - | SD - | C.V. < 5 |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
Multi
- Storage information
Please refer to protocols
Notes
8-hydroxy 2 deoxyguanosine ELISA Kit (8-OHdG) (ab201734) is a competitive assay that can be used for the quantification of 8-OHdG in urine, cell culture, plasma, and other sample matrices. The ELISA utilizes an 8-hydroxy-2-deoxyguanosine-coated plate and an HRP-conjugated antibody for detection which allows for an assay range of 0.94 - 60 ng/mL, with a sensitivity of 0.59 ng/mL. The other highlights of this kit are a quick incubation time of 60 minutes, stable reagents, and an easy to use protocol.
It is important to note that the 8-OHdG antibody used in this assay recognizes both free 8-OHdG and DNA-incorporated 8-OHdG. Since complex samples such as plasma, cell lysates, and tissues are comprised of mixtures of DNA fragments and free 8-OHdG, concentrations of 8-OHdG reported by ELISA methodology will not coincide with those reported by LC-MS where the single nucleoside is typically measured. This should be kept in mind when analyzing and interpreting experimental results.
8-hydroxy-2-deoxy Guanosine (8-OHdG) is produced by the oxidative damage of DNA by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress. Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OHdG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension.
In complex samples such as plasma, cell lysates, and tissues, 8-OHdG can exist as either the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OHdG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OHdG than plasma. Urinary levels of 8-OHdG range between 2.7- 13 ng/mg creatine, while plasma levels of free 8-OHdG have been reported to be between 4-21 pg/mL as determined by LC-MS.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
8-Hydroxy-2'-deoxyguanosine (8-OHdG) also known as 8-hydroxyguanine or simply 8-OHdG is a well-known marker of oxidative DNA damage. This compound forms through the oxidative modification of deoxyguanosine a nucleoside component of DNA mainly by reactive oxygen species (ROS). 8-OHdG is a stable product with a molecular mass of 283.26 g/mol. It is found in various tissues and is excreted in urine which makes it a useful non-invasive biomarker for assessing oxidative stress levels in the body.
- Biological function summary
8-hydroxy-2-deoxyguanosine plays a significant role in indicating cellular oxidative damage but it doesn't act as a signaling molecule or enzyme. Instead it serves as a biomarker that results from the damage. Oxidative stress caused by reactive oxygen species can lead to the formation of 8-OHdG and indicates potential mutations that may arise if the damage goes unrepaired. Its formation does not involve complex protein assemblies but results from the attack on DNA by ROS highlighting cellular environments exposed to oxidative conditions.
- Pathways
Several factors cause oxidative stress leading to the generation of 8-OHdG linking it to key biological pathways such as the DNA damage response and repair pathways. The base excision repair (BER) pathway is important for recognizing and repairing the damage introduced by 8-OHdG. Proteins such as 8-oxoguanine glycosylase (OGG1) directly interact with 8-OHdG during the repair process excising the damaged base from DNA to prevent mutations and maintain genomic integrity.
- Associated diseases and disorders
Researchers associate elevated levels of 8-OHdG with various conditions linked to oxidative stress such as cancer and neurodegenerative diseases like Alzheimer's disease. In cancer the accumulation of 8-OHdG can provide evidence of increased oxidative DNA damage which may lead to mutations and carcinogenesis. Alzheimer’s disease patients often exhibit elevated 8-OHdG levels indicating oxidative damage in neural tissues. The protein OGG1 plays a pivotal role in relationship with these diseases serving as an important enzyme for repairing the 8-OHdG lesions and potentially impacting disease progression.
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8 product images
Competitive ELISA - 8-hydroxy 2 deoxyguanosine ELISA Kit (ab201734)
8-hydroxy 2 deoxyguanosine measured in urine (dilution test range: 1/10-1/200; duplicates; +/- SD).
Competitive ELISA - 8-hydroxy 2 deoxyguanosine ELISA Kit (ab201734)
Standard curve with background signal subtracted (duplicates; +/- SD).
Competitive ELISA - 8-hydroxy 2 deoxyguanosine ELISA Kit (ab201734)
Abcam's 8-hydroxy 2 deoxyguanosine ELISA Kit (ab201734) Typical standard curve
Competitive ELISA - 8-hydroxy 2 deoxyguanosine ELISA Kit (ab201734)
Abcam's 8-hydroxy 2 deoxyguanosine ELISA Kit (ab201734) Schematic
Competitive ELISA - 8-hydroxy 2 deoxyguanosine ELISA Kit (ab201734)
Abcam's 8-hydroxy 2 deoxyguanosine ELISA Kit (ab201734) Recovery of 8-hydroxy-2-deoxy Guanosine from urine
Competitive ELISA - 8-hydroxy 2 deoxyguanosine ELISA Kit (ab201734)
Abcam's 8-hydroxy 2 deoxyguanosine ELISA Kit (ab201734) Oxidation of Guanosine
Biochemical assay - 8-hydroxy 2 deoxyguanosine ELISA Kit (ab201734)
Chen et al used Lipid Peroxidation (MDA) Assay Kit Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric) ab118970, 3-Nitrotyrosine Kit 3-Nitrotyrosine ELISA Kit ab116691, and 8-OHdG ELISA kit ab201734 to investigate oxidative stress in spinal cord injury in mice.
FGF5 overexpression reduces inflammation and oxidative stress in mice treated with surgery to create spinal cord injury. Yellow = control. Green = FGF5 overexpression. Mice were overexpressed with FGF5 using lentiviral vectors, and then were exposed to SCI or sham surgery 2 weeks post‐injection. The levels of MDA, 3‐NT and 8‐OHdG. n = 6 for each groups. Data are expressed as the mean ± standard deviation and p < 0.05 was considered statistically significant. *p < 0.05.
Levels in the spinal cord of malondialdehyde (MDA), 3‐nitrotyrosine (3‐NT) and 8‐hydroxy 2 deoxyguanosine (8‐OHdG) were detected using commercial kits (#Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric) ab118970 for MDA, #3-Nitrotyrosine ELISA Kit ab116691 for 3‐NT, #ab201734 for 8‐OHdG; Abcam) to evaluate oxidative damage of lipids, proteins and nucleic acids according to the manufacturer's instructions.
Competitive ELISA - 8-hydroxy 2 deoxyguanosine ELISA Kit (ab201734)
Example of 8-hydroxy 2 deoxyguanosine standard curve.
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