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AB133025

8-iso-PGF2 alpha ELISA Kit

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8-iso-PGF2 alpha ELISA Kit is a Competitive ELISA for the measurement of 8-iso-PGF2 alpha in in Biofluids, Cell/Tissue Extracts, Cell Culture Media samples.
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Competitive ELISA - 8-iso-PGF2 alpha ELISA Kit (AB133025)
  • cELISA

Supplier Data

Competitive ELISA - 8-iso-PGF2 alpha ELISA Kit (AB133025)

Representative Standard Curve using ab133025

Key facts

Detection method

Colorimetric

Sample types

Urine, Tissue, Cell culture supernatant

Assay type

Competitive

Sensitivity

= 16.3 pg/mL

Range

6.1 - 100000 pg/mL

Assay time

3h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's 8-iso-PGF2 alpha in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 8-iso-PGF2 alpha in tissue culture media and urine.

A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-8-iso-PGF2 alpha antigen and a polyclonal rabbit antibody specific to 8-iso-PGF2 alpha. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of 8-iso-PGF2 alpha captured in the plate.

The measurement of F2-isoprostanes has emerged as one of the most reliable approaches to assess oxidative stress status in vivo, providing an important tool to explore the role of oxidative stress in the pathogenesis of human disease. This is a colorimetric competitive immunoassay kit for the quantitative determination of free 8-iso-Prostaglandin F2α in biological fluids such as urine and in culture supernatants. 8-iso-PGF2α is produced in vivo by both non-cyclooxygenase and cyclooxygenase dependant mechanisms from arachidonic acid. It has been shown to be a potent vasoconstrictor, a potential mediator of hepatorenal syndrome and a mutagen in 3T3 cells and vascular smooth muscle cells. 8-iso-PGF2α has been shown to circulate in plasma and is excreted in urine.

Compound Cross Reactivity
8-iso-PGF2α 100%
PGF1α 4.6%
PGF2α 1.85%
PGE1 0.19%
TXB2 0.023%
PGB1 0.02%
PGE3 0.012%
6-keto-PGF1α 0.008%
13, 14-dihydro-15-keto-PGF2α 0.005%
6,15-keto-13, 14-dihydro-PGF1α <0.001%
8-iso-PGE1 <0.001%
PGA2 <0.001%
PGJ2 <0.001%
2-Arachidonoylglycerol <0.001%
Anandamide <0.001%
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Precision

[ { "reproducibilityType": "Inter", "sample": "High", "replicates": 8, "mean": "6239 pg/mL", "standardDeviation": null, "coefficientOfVariability": "5" }, { "reproducibilityType": "Inter", "sample": "Low", "replicates": 8, "mean": "183 pg/mL", "standardDeviation": null, "coefficientOfVariability": "11" }, { "reproducibilityType": "Inter", "sample": "Media", "replicates": 8, "mean": "855 pg/mL", "standardDeviation": null, "coefficientOfVariability": "8.8" }, { "reproducibilityType": "Intra", "sample": "High", "replicates": 12, "mean": "8263 pg/mL", "standardDeviation": null, "coefficientOfVariability": "5.8" }, { "reproducibilityType": "Intra", "sample": "Low", "replicates": 12, "mean": "232 pg/mL", "standardDeviation": null, "coefficientOfVariability": "11" }, { "reproducibilityType": "Intra", "sample": "Media", "replicates": 12, "mean": "1128 pg/mL", "standardDeviation": null, "coefficientOfVariability": "4.4" } ]
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Recovery

[ { "sample": "Urine", "range": null, "average": "= 101.58" }, { "sample": "Tissue Culture Media", "range": null, "average": "= 129.9" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab133025-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Sealer", "number":"AB133025-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Goat anti-rabbit IgG Microplate (12 x 8 wells)", "number":"AB133025-CMP09", "productcode":"" }, { "size":"1 x 27 mL", "name":"20X Wash Buffer Concentrate", "number":"AB133025-CMP08", "productcode":"" }, { "size":"1 x 500 µL", "name":"8-iso-PGF2 alpha Standard", "number":"AB133025-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"pNpp Substrate", "number":"AB133025-CMP03", "productcode":"" }, { "size":"1 x 5 mL", "name":"Stop Solution", "number":"AB133025-CMP04", "productcode":"" }, { "size":"1 x 27 mL", "name":"10X Assay Buffer", "number":"AB133025-CMP01", "productcode":"" }, { "size":"1 x 5 mL", "name":"8-iso-PGF2 alpha EIA Antibody", "number":"AB133025-CMP06", "productcode":"" }, { "size":"1 x 5 mL", "name":"8-iso-PGF2 alpha Alkaline Phosphatase Conjugate", "number":"AB133025-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

8-iso-PGF2α also known as 8-iso-PGF2a iso-8-PGF2 or PGF2 alpha is an isoprostane formed by the free-radical-catalyzed peroxidation of arachidonic acid. It has a mass of approximately 354 g/mol. This molecule is commonly found in cell membranes during oxidative stress. It exists in various tissues with higher expressions noted in the liver heart and brain. 8-iso-PGF2α can be detected using methods such as the Alpha ELISA or Iso Kit which are critical for measuring its levels in biological samples.
Biological function summary

8-iso-PGF2α functions as a marker of oxidative stress within the organism. It participates in cell signaling affecting vascular tone and platelet aggregation. It does not form part of a larger protein complex by itself but acts through receptor-mediated mechanisms mainly interacting with thromboxane receptors to exert its effects. This interaction leads to vasoconstriction and plays roles in various cell signaling pathways.

Pathways

8-iso-PGF2α is involved in the oxidative stress response and the arachidonic acid metabolism pathways. It closely relates to the cyclooxygenase (COX) pathway intersecting with proteins such as COX-2 and modulating inflammatory responses. This interaction can influence the production of other prostanoids which further integrate into the inflammatory response pathways.

8-iso-PGF2α serves as a biomarker for conditions where oxidative stress is increased such as atherosclerosis and Alzheimer's disease. It connects to diseases through its impact on oxidative stress markers and its influence on vascular health. The molecule's interaction with thromboxane receptors and COX-2 makes it relevant in the progression of these diseases highlighting its potential as a therapeutic target for modulating oxidative damage in related conditions.
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Product protocols

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Target data

See full target information 8-iso-PGF2a
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websiteProtocolBooklet
en
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Product promise

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