8-iso-PGF2 alpha ELISA Kit is a Competitive ELISA kit for the measurement of 8-iso-PGF2 alpha in Urine, Tissue, Cell culture supernatant samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Tissue, Cell culture supernatant
- Assay type
- Competitive
- Range
- 6.1 - 100000 pg/mL
- Assay time
- 3h
- Sensitivity
- = 16.3 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application cELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Alternative names
8-iso-Prostaglandin F2a
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8-iso-PGF2 alpha ELISA Kit is a Competitive ELISA kit for the measurement of 8-iso-PGF2 alpha in Urine, Tissue, Cell culture supernatant samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Tissue, Cell culture supernatant
- Assay type
- Competitive
- Range
- 6.1 - 100000 pg/mL
- Assay time
- 3h
- Assay Platform
- Microplate
- Sensitivity
- = 16.3 pg/mL
Precision
Intra assay
| Sample | n | mean | C.V. |
|---|---|---|---|
Sample High | n 12 | mean 8263 pg/mL | C.V. 5.8 |
Sample Low | n 12 | mean 232 pg/mL | C.V. 11 |
Sample Media | n 12 | mean 1128 pg/mL | C.V. 4.4 |
Inter assay
| Sample | n | mean | C.V. |
|---|---|---|---|
Sample High | n 8 | mean 6239 pg/mL | C.V. 5 |
Sample Low | n 8 | mean 183 pg/mL | C.V. 11 |
Sample Media | n 8 | mean 855 pg/mL | C.V. 8.8 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Urine | Average % = 101.58 | Range |
Sample type Tissue Culture Media | Average % = 129.9 | Range |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Abcam's 8-iso-PGF2 alpha in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 8-iso-PGF2 alpha in tissue culture media and urine.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-8-iso-PGF2 alpha antigen and a polyclonal rabbit antibody specific to 8-iso-PGF2 alpha. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of 8-iso-PGF2 alpha captured in the plate.
The measurement of F2-isoprostanes has emerged as one of the most reliable approaches to assess oxidative stress status in vivo, providing an important tool to explore the role of oxidative stress in the pathogenesis of human disease. This is a colorimetric competitive immunoassay kit for the quantitative determination of free 8-iso-Prostaglandin F2α in biological fluids such as urine and in culture supernatants. 8-iso-PGF2α is produced in vivo by both non-cyclooxygenase and cyclooxygenase dependant mechanisms from arachidonic acid. It has been shown to be a potent vasoconstrictor, a potential mediator of hepatorenal syndrome and a mutagen in 3T3 cells and vascular smooth muscle cells. 8-iso-PGF2α has been shown to circulate in plasma and is excreted in urine.
| Compound | Cross Reactivity |
| 8-iso-PGF2α | 100% |
| PGF1α | 4.6% |
| PGF2α | 1.85% |
| PGE1 | 0.19% |
| TXB2 | 0.023% |
| PGB1 | 0.02% |
| PGE3 | 0.012% |
| 6-keto-PGF1α | 0.008% |
| 13, 14-dihydro-15-keto-PGF2α | 0.005% |
| 6,15-keto-13, 14-dihydro-PGF1α | <0.001% |
| 8-iso-PGE1 | <0.001% |
| PGA2 | <0.001% |
| PGJ2 | <0.001% |
| 2-Arachidonoylglycerol | <0.001% |
| Anandamide | <0.001% |
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
8-iso-PGF2α also known as 8-iso-PGF2a iso-8-PGF2 or PGF2 alpha is an isoprostane formed by the free-radical-catalyzed peroxidation of arachidonic acid. It has a mass of approximately 354 g/mol. This molecule is commonly found in cell membranes during oxidative stress. It exists in various tissues with higher expressions noted in the liver heart and brain. 8-iso-PGF2α can be detected using methods such as the Alpha ELISA or Iso Kit which are critical for measuring its levels in biological samples.
- Biological function summary
8-iso-PGF2α functions as a marker of oxidative stress within the organism. It participates in cell signaling affecting vascular tone and platelet aggregation. It does not form part of a larger protein complex by itself but acts through receptor-mediated mechanisms mainly interacting with thromboxane receptors to exert its effects. This interaction leads to vasoconstriction and plays roles in various cell signaling pathways.
- Pathways
8-iso-PGF2α is involved in the oxidative stress response and the arachidonic acid metabolism pathways. It closely relates to the cyclooxygenase (COX) pathway intersecting with proteins such as COX-2 and modulating inflammatory responses. This interaction can influence the production of other prostanoids which further integrate into the inflammatory response pathways.
- Associated diseases and disorders
8-iso-PGF2α serves as a biomarker for conditions where oxidative stress is increased such as atherosclerosis and Alzheimer's disease. It connects to diseases through its impact on oxidative stress markers and its influence on vascular health. The molecule's interaction with thromboxane receptors and COX-2 makes it relevant in the progression of these diseases highlighting its potential as a therapeutic target for modulating oxidative damage in related conditions.
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Competitive ELISA - 8-iso-PGF2 alpha ELISA Kit (ab133025)
Representative Standard Curve using ab133025
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