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AB133043

8-iso-PGF2alpha ELISA Kit

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(2 Publications)

8-iso-PGF2alpha ELISA Kit is a Competitive ELISA for the measurement of 8-iso-PGF2alpha in in Biofluids, Cell/Tissue Extracts samples.
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Competitive ELISA - 8-iso-PGF2alpha ELISA Kit (AB133043)
  • cELISA

Supplier Data

Competitive ELISA - 8-iso-PGF2alpha ELISA Kit (AB133043)

Typical Standard Curve - Overnight Format.

Representative Standard Curve using ab133043. Overnight Format.

Competitive ELISA - 8-iso-PGF2alpha ELISA Kit (AB133043)
  • cELISA

Supplier Data

Competitive ELISA - 8-iso-PGF2alpha ELISA Kit (AB133043)

Typical Standard Curve - 2 Hour Format.

Representative Standard Curve using ab133043. 2 Hour Format.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue, Serum

Assay type

Competitive

Sensitivity

= 40 pg/mL

Range

160 - 100000 pg/mL

Assay time

3h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Direct 8-iso-PGF2alpha in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 8-iso-PGF2α in Biological fluids.

A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-8-iso-PGF2alpha antigen and a rabbit polyclonal antibody specific to 8-iso-PGF2alpha. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of 8-iso-PGF2alpha captured in the plate.

The 8-epimer of Prostaglandin F2α (8-iso-PGF2α) is produced in vivo by both non-cyclooxygenase and cyclooxygenase dependent mechanisms from arachidonic acid. 8-iso-PGF2α has been shown to be a potent vasoconstrictor, a potential mediator of hepatorenal syndrome and atherosclerosis and a mutagen in 3T3 cells and in vascular smooth muscle cells. It has also been postulated to participate as a pathophysiological mediator and is able to modify the fluidity and integrity of membranes. 8-isoPGF2α has been shown to circulate in plasma and is excreted in urine. Methods for assessing total 8-iso-PGF2α typically require the alkaline hydrolysis of 8-iso-PGF2α esters from tissues, followed by length procedures involving extractions, phase separations and thin layer chromatography.

Cross Reactivity

Compound % Cross Reactivity
8-iso-PGF2α 100
PGF1α 4.6
PGF2α 1.85
PGE1 0.19
TXB2 0.023
PGB1 0.02
PGE3 0.012
6-keto-PGF1α 0.008
13,14-dihydro-15-keto-PGF2α 0.008
6,15-keto-13,14-dihydro-PGF1α 0.005
8-iso-PGE1 <0.001
PGA2 <0.001
PGJ2 <0.001
2-Arachidonoylglycerol <0.001
Anandamide <0.001
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Precision

[ { "reproducibilityType": "Inter", "sample": "1473 pg/mL Spike", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.4" }, { "reproducibilityType": "Inter", "sample": "4501 pg/mL Spike", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.8" }, { "reproducibilityType": "Inter", "sample": "6679 pg/mL Spike", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.4" }, { "reproducibilityType": "Intra", "sample": "3264 pg/mL Spike", "replicates": 16, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.7" }, { "reproducibilityType": "Intra", "sample": "5092 pg/mL Spike", "replicates": 16, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.1" }, { "reproducibilityType": "Intra", "sample": "970 pg/mL Spike", "replicates": 16, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.3" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": "= 93.8" }, { "sample": "EDTA Plasma", "range": null, "average": "= 109.8" }, { "sample": "Heparin Plasma", "range": null, "average": "= 97.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab133043-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Sealer", "number":"AB133043-CMP02", "productcode":"" }, { "size":"1 x 5 mL", "name":"Neutralizing Reagent", "number":"AB133043-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Goat anti-rabbit IgG Microplate (12 x 8 wells)", "number":"AB133043-CMP10", "productcode":"" }, { "size":"1 x 27 mL", "name":"20X Wash Buffer Concentrate", "number":"AB133043-CMP09", "productcode":"" }, { "size":"1 x 5 mL", "name":"Direct 8-iso-PGF2 alpha Antibody", "number":"AB133043-CMP05", "productcode":"" }, { "size":"1 x 0.5 mL", "name":"8-iso-PGF2 alpha Standard", "number":"AB133043-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"pNpp Substrate", "number":"AB133043-CMP03", "productcode":"" }, { "size":"1 x 5 mL", "name":"Direct 8-iso-PGF2 alpha Alkaline Phosphatase Conjugate", "number":"AB133043-CMP06", "productcode":"" }, { "size":"1 x 27 mL", "name":"Direct 8-iso-PGF2 alpha Sample Diluent", "number":"AB133043-CMP07", "productcode":"" }, { "size":"1 x 5 mL", "name":"Stop Solution", "number":"AB133043-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

8-iso-PGF2α also known as 8-iso-PGF2a iso-8-PGF2 or PGF2 alpha is an isoprostane formed by the free-radical-catalyzed peroxidation of arachidonic acid. It has a mass of approximately 354 g/mol. This molecule is commonly found in cell membranes during oxidative stress. It exists in various tissues with higher expressions noted in the liver heart and brain. 8-iso-PGF2α can be detected using methods such as the Alpha ELISA or Iso Kit which are critical for measuring its levels in biological samples.
Biological function summary

8-iso-PGF2α functions as a marker of oxidative stress within the organism. It participates in cell signaling affecting vascular tone and platelet aggregation. It does not form part of a larger protein complex by itself but acts through receptor-mediated mechanisms mainly interacting with thromboxane receptors to exert its effects. This interaction leads to vasoconstriction and plays roles in various cell signaling pathways.

Pathways

8-iso-PGF2α is involved in the oxidative stress response and the arachidonic acid metabolism pathways. It closely relates to the cyclooxygenase (COX) pathway intersecting with proteins such as COX-2 and modulating inflammatory responses. This interaction can influence the production of other prostanoids which further integrate into the inflammatory response pathways.

8-iso-PGF2α serves as a biomarker for conditions where oxidative stress is increased such as atherosclerosis and Alzheimer's disease. It connects to diseases through its impact on oxidative stress markers and its influence on vascular health. The molecule's interaction with thromboxane receptors and COX-2 makes it relevant in the progression of these diseases highlighting its potential as a therapeutic target for modulating oxidative damage in related conditions.
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Product protocols

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Target data

See full target information 8-iso-PGF2a
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Arteriosclerosis, thrombosis, and vascular biology 39:2303-2319 PubMed31510791

2019

ALDH2 (Aldehyde Dehydrogenase 2) Protects Against Hypoxia-Induced Pulmonary Hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zhao,Bailu Wang,Jian Zhang,Dayu He,Qun Zhang,Chang Pan,Qiuhuan Yuan,Yinan Shi,Haiyang Tang,Feng Xu,Shujian Wei,Yuguo Chen

Journal of diabetes investigation 10:1189-1198 PubMed30825261

2019

Glycine increases glyoxalase-1 function by promoting nuclear factor erythroid 2-related factor 2 translocation into the nucleus of kidney cells of streptozotocin-induced diabetic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ziwei Wang,Dan Zhao,Lei Chen,Jingjing Li,Geheng Yuan,Guosheng Yang,Hong Zhang,Xiaohui Guo,Junqing Zhang
View all publications
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